3.6.1.23 additional information - additional information detailed pre-steady-state and steady-state kinetic analysis, quantitative kinetic model of the human dUTPase catalytic cycle, overview 687636 3.6.1.23 additional information - additional information kinetics of dUTP binding 670432 3.6.1.23 additional information - additional information ligand binding constants 689949 3.6.1.23 additional information - additional information lower affinity for dUTP than strict dUTPases 648212 3.6.1.23 additional information - additional information Michaelis-Menten and ligand binding kinetics, overview. Thermodynamic parameters for the interaction between Plasmodium falciparum dUTPase and deoxyuridine derivatives at 25.2 °C and pH 7.0 696412 3.6.1.23 additional information - additional information Michaelis-Menten kinetics, kinetics analysis 699236 3.6.1.23 additional information - additional information non-cooperative binding to 2'-dUMP, 1 molecule per enzyme subunit, kinetics and thermodynamics from isothermal titration microcalorimetry under different conditions, overview 667783 3.6.1.23 additional information - additional information steady-state kinetic analysis of the dual activities of the bifunctional enzyme, overview 688399 3.6.1.23 additional information - additional information transient state kinetics of substrate binding to the S72A mutant dUTPase, stopped-flow measurements, overview. Comparative kinetics of formation of the enzyme-substrate complexes of the wild-type and S72A 696282 3.6.1.23 0.0001 - dUTP - 209966 3.6.1.23 0.00017 - dUTP pH and temperature not specified in the publication 712596 3.6.1.23 0.00018 - dUTP - 209986 3.6.1.23 0.0002 - dUTPalphaS - 209998 3.6.1.23 0.00022 - dUTP pH and temperature not specified in the publication 712596 3.6.1.23 0.0003 - dUTP - 209998 3.6.1.23 0.0004 - dUTP at pH 7.6 and 25°C 756317 3.6.1.23 0.0004 - dUTP pH 7.5, 25°C 656212 3.6.1.23 0.0005 - dUTP 25°C 655557 3.6.1.23 0.0006 - dUTP mutant enzyme C4S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C 712381 3.6.1.23 0.0006 - dUTP pH 7.5, 25°C, recombinant enzyme 670775 3.6.1.23 0.00066 - dUTP pH 8.0, 25°C, recombinant enzyme 699236 3.6.1.23 0.0007 - dUTP pH 7.6, 25°C, recombinant enzyme, in presence of 1 mM MgCl2 670912 3.6.1.23 0.00071 - dUTP wild type enzyme, without MgCl2, with dithiothreitol, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C 712381 3.6.1.23 0.0008 - dUTP wild type enzyme, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C 712381 3.6.1.23 0.0009 - dUTP wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C 720865 3.6.1.23 0.00093 - dUTP wild type enzyme, at pH 7.5 and 20°C 757196 3.6.1.23 0.00094 - dUTP - 209987 3.6.1.23 0.00096 - dUTP pH and temperature not specified in the publication 712596 3.6.1.23 0.001 - dUTP - 209965, 209977 3.6.1.23 0.001 - dUTP wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C 713147 3.6.1.23 0.0011 - dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.0011 - dUTP DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C 720865 3.6.1.23 0.0011 - dUTP mutant enzyme R140K/H145W, at pH 7.5 and 20°C 757063 3.6.1.23 0.0011 - dUTP pH 6.2-8.0 209990 3.6.1.23 0.0012 - dUTP recombinant enzyme 670999 3.6.1.23 0.0013 - dUTP mutant enzyme H145W, at pH 7.5 and 20°C 757063 3.6.1.23 0.0014 - dUTP wild type enzyme, at pH 7.5 and 20°C 757063 3.6.1.23 0.0015 - dUTP in presence of Mg2+, at low concentrations of dUTP 209960 3.6.1.23 0.0016 - dUTP wild type enzyme, at pH 8.0 and 25°C 719408 3.6.1.23 0.0017 - dUTP mutant enzyme I117A, at pH 8.0 and 25°C 719408 3.6.1.23 0.0017 - dUTP wild type enzyme, at pH 7.5 and 20°C 757196 3.6.1.23 0.00173 - 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.00179 - dUTP pH 7.4, 37°C, recombinant enzyme 698310 3.6.1.23 0.0018 - dUTP at pH 7.5 and 20°C 756697 3.6.1.23 0.0018 - dUTP mutant enzyme F46A, at pH 8.0 and 25°C 719408 3.6.1.23 0.00187 - dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.0019 - dUTP mutant enzyme K96A, at pH 8.0 and 25°C 719408 3.6.1.23 0.0019 - dUTP pH 7.0, 25°C, recombinant enzyme 696412 3.6.1.23 0.0019 - dUTP-Mg2+ pH 7.0, 25°C, recombinant enzyme 696412 3.6.1.23 0.002 - dUTP - 209963 3.6.1.23 0.002 - dUTP mutant enzyme V160D, at pH 7.5 and 20°C 757196 3.6.1.23 0.0021 - dUTP mutant enzyme D170G, at pH 7.5 and 20°C 757196 3.6.1.23 0.0021 - dUTP pH 8, 25°C 657286 3.6.1.23 0.0024 - dUTP pH 5.7-6.2, recombinant enzyme 670909 3.6.1.23 0.0025 - dUTP enzyme form DUT-N and DUT-M 209988 3.6.1.23 0.0025 - dUTP mutant enzyme K150A, at pH 7.5 and 20°C 757196 3.6.1.23 0.0026 - dUTP mutant enzyme K150E, at pH 7.5 and 20°C 757196 3.6.1.23 0.0026 - dUTP mutant enzyme R158A, at pH 7.5 and 20°C 757196 3.6.1.23 0.0026 - dUTP mutant enzyme R158E, at pH 7.5 and 20°C 757196 3.6.1.23 0.0027 - dUTP-Co2+ pH 7.0, 25°C, recombinant enzyme 696412 3.6.1.23 0.0028 - dUTP mutant enzyme DELTA170-171, at pH 7.5 and 20°C 757196 3.6.1.23 0.0029 - dUTP mutant enzyme R24T, at pH 7.5 and 20°C 757196 3.6.1.23 0.003 - dUTP - 209969, 209972 3.6.1.23 0.0032 - dUTP mutant enzyme R158W, at pH 7.5 and 20°C 757196 3.6.1.23 0.0036 - dUTP - 209978 3.6.1.23 0.0036 - dUTP mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C 713147 3.6.1.23 0.0036 - dUTP pH 7.4, 20°C, recombinant wild-type enzyme 687636 3.6.1.23 0.0037 - dUTP in presence of 1 mM Mg2+ 209993 3.6.1.23 0.004 - dUTP enzyme from uninfected KB cells 209974 3.6.1.23 0.004 - dUTP in absence of Mg2+, at low concentrations of dUTP 209960 3.6.1.23 0.004 - dUTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C 756316 3.6.1.23 0.0042 - dUTP mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C 757196 3.6.1.23 0.0045 - dUTP - 209973, 209985 3.6.1.23 0.0045 - dUTP mutant enzyme S115R, at pH 7.5 and 20°C 757196 3.6.1.23 0.0048 - dUTP enzyme from herpes simplex virus type 1 infected KB cells 209974 3.6.1.23 0.005 - dUTP mutant enzyme F166Y, at pH 7.5 and 20°C 757196 3.6.1.23 0.0052 - dUTP mutant enzyme D131S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C 712381 3.6.1.23 0.0052 - dUTP mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C 713147 3.6.1.23 0.0052 - dUTP mutant enzyme R166S, at pH 7.5 and 20°C 757196 3.6.1.23 0.0054 - dUTP mutant enzyme R161K, at pH 7.5 and 20°C 757196 3.6.1.23 0.006 - dUTP - 209997 3.6.1.23 0.006 - dUTP wild type enzyme, without MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C 712381 3.6.1.23 0.0061 - 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.0062 - dUDP 25°C 655557 3.6.1.23 0.0067 - dUTP mutant enzyme R24T/S115R, at pH 7.5 and 20°C 757196 3.6.1.23 0.0067 - dUTP mutant enzyme T138STOP, at pH 7.5 and 20°C 757063 3.6.1.23 0.007 - dUTP mutant enzyme D131N, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C 712381 3.6.1.23 0.0071 - dUTP mutant enzyme D170L, at pH 7.5 and 20°C 757196 3.6.1.23 0.0073 - dUTP in the presence of 5 mM Mn2+, at pH 8.2 and 25°C 756316 3.6.1.23 0.008 - dUTP - 209960 3.6.1.23 0.008 - dUTP mutant enzyme R268A, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C 712381 3.6.1.23 0.0082 - dUTP mutant enzyme G143STOP, at pH 7.5 and 20°C 757063 3.6.1.23 0.009 - dUTP mutant enzyme D131S/G78D, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C 712381 3.6.1.23 0.0094 - dATP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C 756316 3.6.1.23 0.0117 - dUTP pH 8.5, 50°C, recombinant enzyme 670091 3.6.1.23 0.012 - dUTP - 209964, 209968, 209980 3.6.1.23 0.013 - dUTP mutant enzyme D34N, at pH 7.5 and 20°C 757196 3.6.1.23 0.013 - dUTP mutant enzyme T22R/R116S, at pH 7.5 and 20°C 757196 3.6.1.23 0.0132 - dUTP wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C 718787 3.6.1.23 0.0137 - dUTP-Mn2+ pH 7.0, 25°C, recombinant enzyme 696412 3.6.1.23 0.0142 - dUTP mutant enzyme Q114A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C 718787 3.6.1.23 0.016 - dUTP - 209986 3.6.1.23 0.021 - dUTP mutant enzyme DELTA134-153, at pH 7.5 and 20°C 757196 3.6.1.23 0.025 - dUDP - 209986 3.6.1.23 0.0315 - dUTP mutant enzyme R111A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C 718787 3.6.1.23 0.035 - UTP mutant enzyme Y88A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C 718787 3.6.1.23 0.041 - dUTP mutant enzyme Y88A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C 718787 3.6.1.23 0.0447 - dITP wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C 718787 3.6.1.23 0.0627 - dUDP pH 8, 25°C 657286 3.6.1.23 0.077 - dCTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C 756316 3.6.1.23 0.11 - dTTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C 756316 3.6.1.23 0.13 - dUDP - 209986 3.6.1.23 0.17 - dUDP - 209985 3.6.1.23 0.21 - dGTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C 756316 3.6.1.23 0.26 - dTTP - 209990 3.6.1.23 0.29 - dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.4 - dTTP - 209998 3.6.1.23 0.438 - beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.45 - dUTP D90N mutant enzyme, pH 7.8, room temperature 656250 3.6.1.23 0.465 - dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.5 - dUTP wild-type enzyme, pH 7.8, room temperature 656250 3.6.1.23 0.523 - beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.54 - UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.571 - UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.64 - dTTP pH 7.5, 25°C 656212 3.6.1.23 0.687 - dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 0.761 - dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 1 - dCTP - 209998 3.6.1.23 1 - UTP - 209998 3.6.1.23 1.13 - dATP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 1.96 - dGTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 711327 3.6.1.23 2.3 - dCTP pH 7.5, 25°C 656212 3.6.1.23 3.788 - dUTP at pH 8.0 and 37°C 757550