3.5.4.9 additional information - additional information kinetics 654529 3.5.4.9 additional information - additional information the His-tag does not alter the activity and kinetics of the enzymes 654797 3.5.4.9 additional information - additional information the overall forward reaction from mythylene- to formyltetrahydrofolate shows a hydride transfer kinetic isotope effect, whereas the the overall reverse reaction does not 701402 3.5.4.9 0.0045 - 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme G122D 744335 3.5.4.9 0.009 - 10-formyltetrahydrofolate pH 7.0, 23°C 654529 3.5.4.9 0.009 - 5,10-methenyltetrahydrofolate pH 7.0, 23°C 654529 3.5.4.9 0.015 - 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme D121A 744335 3.5.4.9 0.016 - 10-formyltetrahydrofolate reverse reaction, recombinant mutant S197D, substrate 10-formyltetrahydrofolate 654797 3.5.4.9 0.0164 - (6R,S)-methenyltetrahydrofolate - 209736 3.5.4.9 0.018 - 10-formyltetrahydrofolate reverse reaction, recombinant mutant R173K, substrate 10-formyltetrahydrofolate 654797 3.5.4.9 0.019 - methenyltetrahydrofolate forward reaction, recombinant mutant S197D 654797 3.5.4.9 0.02 - 10-formyltetrahydrofolate reverse reaction, recombinant mutant S197T, substrate 10-formyltetrahydrofolate 654797 3.5.4.9 0.021 - methenyltetrahydrofolate forward reaction, recombinant mutant R173A and R173K 654797 3.5.4.9 0.024 - 10-formyltetrahydrofolate reverse reaction, recombinant mutant R173A, substrate 10-formyltetrahydrofolate 654797 3.5.4.9 0.025 - methenyltetrahydrofolate forward reaction, recombinant mutant S197T 654797 3.5.4.9 0.025 - methenyltetrahydrofolate forward reaction, recombinant wild-type enzyme 654797 3.5.4.9 0.026 - 10-formyltetrahydrofolate reverse reaction, recombinant wild-type enzyme, substrate 10-formyltetrahydrofolate 654797 3.5.4.9 0.026 - 5,10-methenyltetrahydrofolate at pH 7.9 and 22°C 735050 3.5.4.9 0.026 - 5,10-methenyltetrahydrofolate pH 7.6, 30°C, wild-type enzyme 744335 3.5.4.9 0.026 - methenyltetrahydrofolate forward reaction, recombinant mutant R173E 654797 3.5.4.9 0.028 - 10-formyltetrahydrofolate reverse reaction, recombinant mutant S197R, substrate 10-formyltetrahydrofolate 654797 3.5.4.9 0.028 - 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme R191E 744335 3.5.4.9 0.031 - (6R,S)-methenyltetrahydrofolate - 209738 3.5.4.9 0.031 - 10-formyltetrahydrofolate reverse reaction, recombinant mutant R173E, substrate 10-formyltetrahydrofolate 654797 3.5.4.9 0.038 - methenyltetrahydrofolate forward reaction, recombinant mutant S197A 654797 3.5.4.9 0.04 - 5,10-methenyltetrahydrofolate - 209728, 209729 3.5.4.9 0.04 - 5,10-methenyltetrahydrofolate pH 7.6, 30°C, mutant enzyme C58Y 744335 3.5.4.9 0.043 - 10-formyltetrahydrofolate reverse reaction, recombinant mutant S197A, substrate 10-formyltetrahydrofolate 654797 3.5.4.9 0.05 - 5,10-methenyltetrahydrofolate 30°C, recombinant mutant R250A 656050 3.5.4.9 0.056 - 10-formyltetrahydrofolate - 392333 3.5.4.9 0.06 - 5,10-methenyltetrahydrofolate 30°C, recombinant wild-type enzyme 656050 3.5.4.9 0.07 - 10-formyltetrahydrofolate - 1505, 209722 3.5.4.9 0.071 - 5,10-methenyltetrahydrofolate 30°C, recombinant loop5G mutant 656050 3.5.4.9 0.072 - 5,10-methenyltetrahydrofolate 30°C, recombinant mutant Y240A 656050 3.5.4.9 0.074 - 5,10-methenyltetrahydrofolate - 209729 3.5.4.9 0.075 - 5,10-methenyltetrahydrofolate - 209728 3.5.4.9 0.077 - 5,10-methenyltetrahydrofolate 30°C, recombinant loop3G mutant 656050 3.5.4.9 0.08 - 5,10-methenyltetrahydrofolate - 209741 3.5.4.9 0.09 - 5,10-methenyltetrahydrofolate - 209722 3.5.4.9 0.1 - 10-formyltetrahydrofolate - 209722 3.5.4.9 0.13 - 5,10-methenyltetrahydrofolate - 209727 3.5.4.9 0.14 - 5,10-methenyltetrahydrofolate mutant K56R 209742 3.5.4.9 0.157 - (6R,S)-5,10-methenyltetrahydrofolate pH 7.6, temperature not specified in the publication 743250 3.5.4.9 0.19 - 5,10-methenyltetrahydrofolate - 209719 3.5.4.9 0.19 - 5,10-methenyltetrahydrofolate pH 7.2, 35°C 209721 3.5.4.9 0.2 - 10-formyltetrahydrofolate - 209728 3.5.4.9 0.23 - methenyltetrahydrofolate forward reaction, recombinant mutant S197R 654797 3.5.4.9 0.25 - 5,10-methenyltetrahydrofolate - 1505, 209722, 209728 3.5.4.9 0.32 - 5,10-methenyltetrahydrofolate mutant S49Q 209742 3.5.4.9 0.34 - 5,10-methenyltetrahydrofolate wild-type DC301 209742 3.5.4.9 0.47 - 10-formyltetrahydrofolate - 209722 3.5.4.9 0.48 - 5,10-methenyltetrahydrofolate mutant Y52F 209742 3.5.4.9 0.52 - 5,10-methenyltetrahydrofolate mutant S49A 209742 3.5.4.9 0.56 - 5,10-methenyltetrahydrofolate mutants C147A, T148A 209742 3.5.4.9 1.22 - 5,10-methenyltetrahydrofolate mutant Y52A 209742 3.5.4.9 1.62 - 5,10-methenyltetrahydrofolate mutant Y52F 209742 3.5.4.9 2.36 - 5,10-methenyltetrahydrofolate mutant C147Q 209742