3.5.4.4 additional information - additional information isozymes ADA1 and ADA2 are kinetically distinct 667870 3.5.4.4 additional information - additional information kinetics 668123 3.5.4.4 additional information - additional information kinetics of the large and the small isozyme 667032 3.5.4.4 additional information - additional information optimization of a spectrometric kinetic measurement method pure enzyme, kinetics in presence of substrate, product and inhibitor, overview 669618 3.5.4.4 additional information - additional information optimization of a spectrometric kinetic measurement method using Hut78 cell extract, kinetics in presence of substrate, product and inhibitor, overview 669618 3.5.4.4 additional information - additional information thermodynamics and kinetics 669846 3.5.4.4 0.000023 - adenosine - 655265 3.5.4.4 0.000046 - adenosine - 655265 3.5.4.4 0.004 - 2'-deoxyadenosine - 209608 3.5.4.4 0.0044 - 5'-methylthioadenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.0087 - 5'-methylthioadenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.00943 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174I 755901 3.5.4.4 0.0095 - 5'-methylthioadenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.0147 - adenosine pH 7.5, 25°C 655734 3.5.4.4 0.016 - 2'-deoxyadenosine - 209637 3.5.4.4 0.01728 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme, wild-type enzyme (refolded) 755901 3.5.4.4 0.01784 - adenosine pH 8.0, temperature not specified in the publication, wild-type enzyme 755901 3.5.4.4 0.02064 - adenosine pH 8.0, temperature not specified in the publication, wild-type enzyme 755901 3.5.4.4 0.021 - adenosine at pH 7.4 and 37°C 733674 3.5.4.4 0.02113 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme L179H 755901 3.5.4.4 0.022 - 5'-methylthioadenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.022 - adenosine - 209608 3.5.4.4 0.023 - adenosine - 209637 3.5.4.4 0.02344 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme C27Q/L227I 755901 3.5.4.4 0.026 - adenosine - 209606, 209619 3.5.4.4 0.026 - adenosine 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM 699651 3.5.4.4 0.028 - adenosine type 2 209607 3.5.4.4 0.029 - adenosine 37°C, pH not specified in the publication 720969 3.5.4.4 0.029 - adenosine type 2-1 209607 3.5.4.4 0.0306 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, wild-type enzyme 755901 3.5.4.4 0.031 - adenosine type 1 209607 3.5.4.4 0.032 - adenine - 696861 3.5.4.4 0.032 - adenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.033 - adenosine - 209633 3.5.4.4 0.034 - 2'-deoxyadenosine - 209622 3.5.4.4 0.034 - 2'-deoxyadenosine pH 7.4, 37°C 719423 3.5.4.4 0.037 - adenosine - 209628 3.5.4.4 0.0385 - adenosine - 209620 3.5.4.4 0.03874 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme M155A 755901 3.5.4.4 0.039 - 2'-deoxyadenosine kidney cortex large isozyme 667032 3.5.4.4 0.04 - 2'-deoxyadenosine - 209624 3.5.4.4 0.04 - 2'-deoxyadenosine pleural fluid large isozyme 667032 3.5.4.4 0.041 - adenosine pH 7.4, 37°C 719423 3.5.4.4 0.042 - 2'-deoxyadenosine blood serum large isozyme 667032 3.5.4.4 0.04203 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174A 755901 3.5.4.4 0.04461 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme C27Q/L227I 755901 3.5.4.4 0.048 - adenosine - 209622 3.5.4.4 0.049 - adenosine - 209617 3.5.4.4 0.05 - adenosine - 209616, 209618 3.5.4.4 0.05 0.07 2'-deoxyadenosine - 209621 3.5.4.4 0.051 - adenosine - 209617 3.5.4.4 0.052 - 2'-deoxyadenosine - 209633 3.5.4.4 0.05256 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme L179H 755901 3.5.4.4 0.053 - 2'-deoxyadenosine - 209617 3.5.4.4 0.053 - adenosine blood serum large isozyme 667032 3.5.4.4 0.055 0.07 adenosine - 209621 3.5.4.4 0.056 - adenosine kidney cortex large isozyme 667032 3.5.4.4 0.05641 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme M155A (refolded) 755901 3.5.4.4 0.057 - 2'-deoxyadenosine - 209617 3.5.4.4 0.057 - adenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.06 - adenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.066 - adenosine El 209605 3.5.4.4 0.07 - adenosine pleural fluid large isozyme 667032 3.5.4.4 0.071 - adenosine wild-type, pH 7.5, 37°C 718893 3.5.4.4 0.072 - adenosine pH and temperature not specified in the publication, mutant enzyme Y136R 727779 3.5.4.4 0.074 - adenosine Es 209605 3.5.4.4 0.075 - adenosine - 209624 3.5.4.4 0.081 - 6-methoxypurinriboside - 209633 3.5.4.4 0.083 - adenosine mutant enzyme D172E, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.087 - adenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.088 - adenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.093 - adenosine spleen small isozyme 667032 3.5.4.4 0.104 - adenosine mutant enzyme D172A, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.115 - 5'-methylthioadenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.12 - adenosine wild type enzyme, in 100 mM Tris-HCl buffer (pH 8.0), temperature not specified in the publication 711215 3.5.4.4 0.12628 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A 755901 3.5.4.4 0.129 - adenosine lung small isozyme 667032 3.5.4.4 0.135 - 2'-deoxyadenosine spleen small isozyme 667032 3.5.4.4 0.144 - adenosine - 655272 3.5.4.4 0.15 - adenosine pH and temperature not specified in the publication, mutant enzyme E150R 727779 3.5.4.4 0.15 - adenosine pH and temperature not specified in the publication, wild-type enzyme 727779 3.5.4.4 0.15897 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A/L179A 755901 3.5.4.4 0.17 - AMP - 209627 3.5.4.4 0.189 - 2'-deoxyadenosine lung small isozyme 667032 3.5.4.4 0.19 - adenosine membrane-bound ADA 697462 3.5.4.4 0.2 - 2'-deoxyadenosine - 209630 3.5.4.4 0.20034 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174A 755901 3.5.4.4 0.22 - 2'-deoxyadenosine - 209627 3.5.4.4 0.22 - adenosine - 209630 3.5.4.4 0.22 - adenosine pH 7.4, 37°C 755774 3.5.4.4 0.22 - adenosine soluble fraction 697462 3.5.4.4 0.22422 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A/L179A 755901 3.5.4.4 0.25326 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176M 755901 3.5.4.4 0.26747 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174I 755901 3.5.4.4 0.277 - 6-chloropurinriboside - 209633 3.5.4.4 0.28928 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A 755901 3.5.4.4 0.29 - 2',3'-isopropylidene adenosine - 209627 3.5.4.4 0.29961 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176M 755901 3.5.4.4 0.31 - 6-Chloropurine riboside - 209606 3.5.4.4 0.32206 - adenosine pH 8.0, temperature not specified in the publication, mutant enzyme F136L 755901 3.5.4.4 0.333 - 2'-deoxyadenosine - 655272 3.5.4.4 0.37605 - 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme F136L 755901 3.5.4.4 0.4 - 2'-deoxyadenosine - 209616 3.5.4.4 0.5 - adenosine pH 7.5, 37°C, ADAII 686577 3.5.4.4 0.5 - cAMP - 209627 3.5.4.4 0.56 - 2'-deoxyadenosine 37°C, pH not specified in the publication 720969 3.5.4.4 0.67 - adenosine - 209627 3.5.4.4 0.715 - adenosine - 209613 3.5.4.4 0.734 - adenosine pH 6.8, 37°C 668123 3.5.4.4 1 - adenosine pH 7.2, 37°C, ADAII 686612 3.5.4.4 1.13 - adenosine pH 7.5, 37°C, Vmax is 2.61 nmol NH3/min/mg protein 719525 3.5.4.4 2 - Adenosine-3'-monophosphate - 209627 3.5.4.4 2.15 - adenosine - 667870 3.5.4.4 6.6 - adenosine - 209631