3.5.1.25 additional information - additional information Michaelis-Mente kinetics. The initial rate measured for NagA has a significant substrate concentration-dependent lag phase which is not influenced by 1 mM Zn2+ and not pH-dependent 753578 3.5.1.25 additional information - additional information Michaelis-Menten kinetics 754179 3.5.1.25 0.029 - N-acetylglucosamine 6-phosphate - 246598 3.5.1.25 0.03 - N-acetylglucosamine 6-phosphate - 246595, 246600 3.5.1.25 0.04 - N-acetyl-D-glucosamine 6-phosphate - 678302 3.5.1.25 0.08 - N-acetyl-D-glucosamine 6-phosphate - 678301 3.5.1.25 0.08 - N-acetyl-D-glucosamine 6-phosphate mutant Q59A 678301 3.5.1.25 0.1 - N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Mn 678301 3.5.1.25 0.15 - N-acetyl-D-glucosamine 6-phosphate mutant E131A 678301 3.5.1.25 0.15 - N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Co 678301 3.5.1.25 0.16 - N-acetyl-D-glucosamine-6-phosphate pH 8.0, 30°C, recombinant enzyme 753578 3.5.1.25 0.2 - N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Cd 678301 3.5.1.25 0.2 - N-thioacetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Cd 678301 3.5.1.25 0.23 - N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Fe 678301 3.5.1.25 0.23 - N-thioacetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Mn 678301 3.5.1.25 0.24 - N-thioacetyl-D-glucosamine 6-phosphate - 678301 3.5.1.25 0.29 - N-formyl-D-glucosamine 6-phosphate - 678301 3.5.1.25 0.3 - N-acetyl-D-glucosamine 6-phosphate - 246589, 246597 3.5.1.25 0.3 - N-acetyl-D-glucosamine 6-phosphate at 10°C 246600 3.5.1.25 0.31 - N-acetyl-D-glucosamine 6-phosphate mutant Q59H 678301 3.5.1.25 0.33 - N-acetyl-D-glucosamine 6-phosphate mutant H143Q 678301 3.5.1.25 0.38 - N-acetylglucosamine 6-phosphate at 30°C 246600 3.5.1.25 0.4 - N-acetyl-D-glucosamine 6-phosphate - 246596 3.5.1.25 0.4 - N-trifluoroacetyl-D-glucosamine 6-phosphate - 678301 3.5.1.25 0.64 - N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Ni 678301 3.5.1.25 0.76 - N-acetyl-D-glucosamine 6-phosphate mutant Y223F 678301 3.5.1.25 0.8 - N-acetyl-D-glucosamine 6-phosphate - 246592 3.5.1.25 0.8 - N-acetyl-D-glucosamine 6-phosphate mutant N61A 678301 3.5.1.25 1 - N-acetyl-D-glucosamine 6-phosphate - 246593 3.5.1.25 1.24 - N-Acetyl-D-galactosamine 6-phosphate - 678301 3.5.1.25 1.7 - N-acetyl-D-glucosamine 6-phosphate mutant N61H 678301 3.5.1.25 2 - N-acetyl-D-glucosamine 6-phosphate mutant K139M 678301 3.5.1.25 2.1 - N-acetyl-D-glucosamine 6-phosphate mutant H143N 678301 3.5.1.25 2.7 - N-acetyl-D-glucosamine 6-phosphate mutant H251N 678301 3.5.1.25 3 - N-acetyl-D-glucosamine-6-phosphate pH 7.0, 37°C, recombinant His6-tagged enzyme 754179 3.5.1.25 3.2 - N-acetyl-D-glucosamine-6-phosphate pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme expressed from Mycobacterium smegmatis 754179 3.5.1.25 3.6 - N-acetyl-D-glucosamine 6-phosphate mutant E131Q 678301 3.5.1.25 4.6 - N-acetylglucosamine 6-sulfate - 246595, 246600 3.5.1.25 4.9 - N-acetyl-D-glucosamine 6-sulfate - 678301 3.5.1.25 5.2 - N-acetyl-D-glucosamine-6-phosphate pH 7.0, 37°C, recombinant wild-type His6-tagged enzyme expressed from Escherichia coli 754179 3.5.1.25 5.9 - N-acetylglucosamine 6-sulfate at 10°C 246600 3.5.1.25 11 - N-acetyl-D-glucosamine 6-sulfate Zn in acitve site changed to Cd 678301 3.5.1.25 12 - N-acetylglucosamine 6-sulfate at 30°C 246600 3.5.1.25 19.1 - N-acetyl-D-mannosamine-6-phosphate pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme expressed from Mycobacterium smegmatis 754179 3.5.1.25 19.3 - N-acetyl-D-mannosamine-6-phosphate pH 7.0, 37°C, recombinant His6-tagged enzyme 754179 3.5.1.25 20 - acetate - 246597 3.5.1.25 20.9 - N-acetyl-D-glucosamine-6-sulfate pH 7.0, 37°C, recombinant His6-tagged enzyme 754179 3.5.1.25 21.4 - N-acetyl-D-glucosamine-6-sulfate pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme expressed from Mycobacterium smegmatis 754179 3.5.1.25 25 - glucosamine 6-phosphate - 246597 3.5.1.25 73 - N-acetylglucosamine - 246595, 246600 3.5.1.25 98 - N-acetylglucosamine at 10°C 246600 3.5.1.25 118.5 - N-acetyl-D-galactosamine-6-phosphate pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme expressed from Mycobacterium smegmatis 754179 3.5.1.25 120 - N-acetylglucosamine at 30°C 246600 3.5.1.25 145.4 - N-acetyl-D-galactosamine-6-phosphate pH 7.0, 37°C, recombinant His6-tagged enzyme 754179