3.4.22.69 additional information - additional information steady-state analysis of the solvent isotope effects on KM-value 678335 3.4.22.69 0.0005 - Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.0011 - Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.0013 - Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.0013 - Abz-VLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.0016 - Abz-SAVLQSGK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.0017 - Abz-LQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.002 - Abz-SAVLQSGFRKMAK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.0024 - Abz-SAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.004 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(303-306) 690620 3.4.22.69 0.005 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q200E 690620 3.4.22.69 0.005 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299N 690620 3.4.22.69 0.006 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(304-306) 690620 3.4.22.69 0.01 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298A/Q299A 690620 3.4.22.69 0.01 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S139A 690620 3.4.22.69 0.011 - Abz-QTSITSAVLQSGFRK(Dnp)NH2 in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.011 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298A 690620 3.4.22.69 0.011 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide wild-type enzyme 690620 3.4.22.69 0.012 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123a/R298A 690620 3.4.22.69 0.013 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(299-306) 690620 3.4.22.69 0.013 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298L 690620 3.4.22.69 0.013 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123A 690620 3.4.22.69 0.013 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123C 690620 3.4.22.69 0.0136 - Abz-SAVLQSGK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.014 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(305-306) 690620 3.4.22.69 0.015 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(300-306) 690620 3.4.22.69 0.016 - Abz-SAVLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.016 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298K 690620 3.4.22.69 0.018 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299A 690620 3.4.22.69 0.0186 - SAVLQMGFRK mutant enzyme T25G, at 37°C 707802 3.4.22.69 0.0186 - Ser-Ala-Val-Leu-Gln-Met-Gly-Phe-Arg-Lys T25G mutant protein 707802 3.4.22.69 0.021 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(297-306) 690620 3.4.22.69 0.021 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S139A/Q299A 690620 3.4.22.69 0.022 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(302-306) 690620 3.4.22.69 0.023 - Abz-LQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.0245 - Dabcyl-KTSAVLQSGFRKME-EDANS-amide in 40 mM Tris-HCl buffer, pH 7.3, temperature not specified in the publication 717101 3.4.22.69 0.03 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(298-306) 690620 3.4.22.69 0.03 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(301-306) 690620 3.4.22.69 0.033 - Abz-VLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.0338 - TSAVLQSGFRK-NH2 pH 7.5, 37°C, mutant enzyme R188I 691242 3.4.22.69 0.037 - o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299K 690620 3.4.22.69 0.03817 - [4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] mutant enzyme Q189A 678590 3.4.22.69 0.039 - N-methylanthranilyl-TSAVLQSGFRK(2,4-dinitrophenyl) pH 7.5, 30°C 755253 3.4.22.69 0.0423 - DABCYL-TSAVLQSGFRKME-EDANS pH not specified in the publication, temperature not specified in the publication 764084 3.4.22.69 0.045 - [4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-5-['-(aminoethyl)amino]-naphthalenesulfonic acid - 678335 3.4.22.69 0.046 - GPFVDRQTAQAAGTDT-NH2 pH 7.5, 37°C, mutant enzyme R188I 691242 3.4.22.69 0.04938 - [4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] wild-type enzyme 678590 3.4.22.69 0.051 - KVATVQSKMSD-NH2 pH 7.5, 37°C, mutant enzyme R188I 691242 3.4.22.69 0.05642 - Dabcyl-KTSAVLQSGFRKME-EDANS wild type enzyme, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C 717244 3.4.22.69 0.06783 - Dabcyl-KTSAVLQSGFRKME-EDANS mutant enzyme N28A, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C 717244 3.4.22.69 0.073 - SITSAVLQ-p-nitrophenyl ester - 678335 3.4.22.69 0.0766 - SAVLQMGFRK wild type enzyme, at 37°C 707802 3.4.22.69 0.0766 - Ser-Ala-Val-Leu-Gln-Met-Gly-Phe-Arg-Lys wild-type protein 707802 3.4.22.69 0.0787 - DABCYL-TSAVLQSGFRKME-EDANS pH not specified in the publication, temperature not specified in the publication 764084 3.4.22.69 0.11 - Dabcyl-KTSAVLQSGFRKME-EDANS wild-type, pH 7.5, 25°C 765793 3.4.22.69 0.146 - o-aminobenzoyl-TSAVLQSGFRY(NO2)G - 678512 3.4.22.69 0.18 - SITSAVLQ-p-nitroanilide - 678335 3.4.22.69 0.216 - Dabcyl-KTSAVLQSGFRKME-EDANS mutant P108S, pH 7.5, 25°C 765793 3.4.22.69 0.2226 - Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide wild-type protein 707753 3.4.22.69 0.286 - TFTRLQSLENV pH 7.3, room temperature 691145 3.4.22.69 0.306 - (Ala-Arg-Leu-Gln-NH)2-rhodamine rate of hydrolysis measured by change in absorbance at 496 nm 668633 3.4.22.69 0.3534 - Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide E166A mutant protein 707753 3.4.22.69 0.549 - FYPKLQASQAW pH 7.3, room temperature 691145 3.4.22.69 0.583 - SGVTFQGKFKK pH 7.3, room temperature 691145 3.4.22.69 0.6 - SITSAVLQSGFRKMA - 678335 3.4.22.69 0.68 - Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide mutant G11A/L141T/R298A/Q299A, pH 7.3, 37°C 755419 3.4.22.69 0.98 - Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide wild-type, pH 7.3, 37°C 755419 3.4.22.69 1.15 - TSAVLQSGFRK-NH2 pH 7.3, room temperature 691145 3.4.22.69 1.44 - ATVRLQAGNAT pH 7.3, room temperature 691145 3.4.22.69 1.94 - PHTVLQAVGAC pH 7.3, room temperature 691145 3.4.22.69 61.9 - SWTSAVLQSGFRKWA HPLC-based cleavage assay, measurement of fluorescence emission at 353 nm 669868