3.4.21.9 0.001 - Trypsinogen bovine guanidinated trypsinogen 95572 3.4.21.9 0.0014 - human cationic trypsinogen 37 °C, 100 mM Tris-HCl pH 8.0, 1mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM human enteropeptidase 669370 3.4.21.9 0.0021 - A4-mutant trypsinogen 37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM human enteropeptidase 669370 3.4.21.9 0.00258 - GD4R-4-nitroanilide mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.0045 - Trypsinogen bovine trypsinogen 95571 3.4.21.9 0.0056 - Trypsinogen pH 5.6 95578 3.4.21.9 0.00629 - GD4R-4-nitroanilide wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.007 - Trypsinogen - 95572 3.4.21.9 0.007 - Trypsinogen bovine trypsinogen 95578 3.4.21.9 0.0072 - human cationic trypsinogen 25 °C, 28 mM sodium succinate (pH 5.6), 10 mM CaCl2 669370 3.4.21.9 0.0072 - Trypsinogen human trypsinogen 95571 3.4.21.9 0.017 - Trypsinogen - 95570 3.4.21.9 0.0179 - GDDDDK-4-nitroanilide mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.018 - GD4R-4-nitroanilide mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.019 - GDDDDK-2-naphthylamide phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C 731383 3.4.21.9 0.02 - GDDDDK-2-naphthylamide soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C 731383 3.4.21.9 0.025 - GD4K 7-amido-4-methylcoumarin pH 8.4, 35°C 717431 3.4.21.9 0.0298 - GDDDDK-4-nitroanilide wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.034 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide pH 8.4, 37°C 718359 3.4.21.9 0.034 - Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 0.02 mM CaCl2 683172 3.4.21.9 0.05 - thiobenzyl benzyloxycarbonyl-L-lysinate mutant enzyme R99A 95577 3.4.21.9 0.0647 - APFDDDDKIVGG EP light chain 684036 3.4.21.9 0.067 - Z-Ala-Phe-Arg-nitroanilide - 683172 3.4.21.9 0.07 - Trypsinogen bovine trypsinogen 95575 3.4.21.9 0.076 - formyl-Ala-Phe-Arg-4-nitroanilide pH 8.4, 25°C 718359 3.4.21.9 0.076 - N-formyl-Ala-Phe-Arg-4-nitroanilide - 683172 3.4.21.9 0.08 - Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 1mM EDTA 683172 3.4.21.9 0.091 - Z-Gly-Pro-Arg-nitroanilide - 683172 3.4.21.9 0.1 - thiobenzyl benzyloxycarbonyl-L-lysinate mutant enzyme K96A 95577 3.4.21.9 0.1 - Z-Phe-Arg-4-methylcoumaryl-7-amide 37°C, pH 7.4 716743 3.4.21.9 0.1 - Z-Phe-Arg-4-methylcoumaryl-7-amide K63R mutant, 37°C, pH 7.4 716743 3.4.21.9 0.1 - Z-Phe-Arg-4-methylcoumaryl-7-amide T105E mutant, 37°C, pH 7.4 716743 3.4.21.9 0.108 - Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide at pH 8.0 and 37°C 752564 3.4.21.9 0.109 - Z-Ala-Tyr-Arg-nitroanilide - 683172 3.4.21.9 0.12 - thiobenzyl benzyloxy-carbonyl-L-lysinate pH 8.4, 25°C 718359 3.4.21.9 0.12 - thiobenzyl benzyloxycarbonyl-L-lysinate - 95577 3.4.21.9 0.12 - thiobenzyl benzyloxycarbonyl-L-lysinate mutant enzyme R97A 95577 3.4.21.9 0.123 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide value obtained by fluorescence spectroscopy, pH not specified in the publication, at 37°C 732551 3.4.21.9 0.125 - PrAD4KP26 in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 0.14 - thiobenzyl benzyloxy-carbonyl-L-lysinate pH 8.4, 25°C 718359 3.4.21.9 0.14 - thiobenzyl benzyloxy-carbonyl-L-lysinate pH 8.4, 37°C 653769 3.4.21.9 0.14 - thiobenzyl benzyloxycarbonyl-L-lysinate - 683172 3.4.21.9 0.14 - thiobenzyl benzyloxycarbonyl-L-lysinate mutant enzyme R98A 95577 3.4.21.9 0.141 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide pH 8.4, 37°C 718359 3.4.21.9 0.141 - Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 0.02 mM CaCl2 683172 3.4.21.9 0.143 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide value obtained by impedance spectroscopy, pH not specified in the publication, at 37°C 732551 3.4.21.9 0.147 - APFDDDDRIVGG at 0.014-0.05 mM, holoenzyme 684036 3.4.21.9 0.16 - GD4KF(NO2)G 50 mM Ca2+, pH 8.0, 37°C 667759 3.4.21.9 0.16 - GD4KF(NO2)G in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 0.16 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide pH 8.4, 37°C 653769 3.4.21.9 0.165 - Z-Lys-SBzl mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.167 - formyl-Ala-Phe-Lys-4-nitroanilide pH 8.4, 25°C 718359 3.4.21.9 0.167 - N-formyl-Ala-Phe-Lys-4-nitroanilide - 683172 3.4.21.9 0.17 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide pH 8.0, 25°C 653747 3.4.21.9 0.172 - Z-Lys-SBzl mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.19 - Z-Lys-SBzl wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.192 - formyl-Ala-Phe-Arg-4-nitroanilide pH 8.4, 25°C 718359 3.4.21.9 0.2 - Boc-Glu(OBzl)-Ala-Arg-MCA wild-type enzyme, 37°C, pH 7.4 716743 3.4.21.9 0.2 - GD4KNfa in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 0.2 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide K63R mutant, 37°C, pH 7.4 716743 3.4.21.9 0.2 - glycyl-tetra-L-aspartyl-L-lysine beta-naphthylamide 50 mM Ca2+, pH 8.0, 37°C 667759 3.4.21.9 0.2 - t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin mutant enzyme E136Y/R213L, at pH 8.3 and 30°C 732855 3.4.21.9 0.2 - Z-Phe-Arg-4-methylcoumaryl-7-amide P193E mutant, 37°C, pH 7.4 716743 3.4.21.9 0.22 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide - 95578 3.4.21.9 0.234 - Z-Ala-Leu-Arg-nitroanilide - 683172 3.4.21.9 0.24 - Z-Ala-Trp-Arg-nitroanilide - 683172 3.4.21.9 0.265 - Z-Pro-Phe-Arg-nitroanilide - 683172 3.4.21.9 0.276 - GDDDDK-4-nitroanilide mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C 732883 3.4.21.9 0.28 - formyl-Ala-Phe-Lys-4-nitroanilide pH 8.4, 25°C 718359 3.4.21.9 0.3 - APFDDDGKIVGG at 0.08-2 mM, holoenzyme 684036 3.4.21.9 0.3 - GDDDDK-2-naphthylamide mutant enzyme E136Y/R213L, at pH 8.3 and 30°C 732855 3.4.21.9 0.3 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide E173A mutant, 37°C, pH 7.4 716743 3.4.21.9 0.332 - Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide in presence of 1mM EDTA 683172 3.4.21.9 0.353 - APFDDDDKIVGG at 0.03-0.08 mM, holoenzyme 684036 3.4.21.9 0.356 - Z-Ala-Ala-Arg-nitroanilide - 683172 3.4.21.9 0.4 - GDDDDK-2-naphthylamide mutant enzyme H24Q, at pH 8.3 and 30°C 732855 3.4.21.9 0.4 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide P193E mutant, 37°C, pH 7.4 716743 3.4.21.9 0.4 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide T105E mutant, 37°C, pH 7.4 716743 3.4.21.9 0.4 - Z-Phe-Arg-4-methylcoumaryl-7-amide E173A mutant, 37°C, pH 7.4 716743 3.4.21.9 0.42 - Z-Ala-Met-Arg-nitroanilide - 683172 3.4.21.9 0.437 - G5DKF(NO2)G in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 0.45 - Val-(Asp)4-Arg-Ile-Val-Gly - 696841 3.4.21.9 0.5 - benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme E136Y/R213L, at pH 8.3 and 30°C 732855 3.4.21.9 0.5 - GDDDDK-2-naphthylamide mutant enzyme R213L, at pH 8.3 and 30°C 732855 3.4.21.9 0.5 0.6 GD4K 2-naphthylamide pH 8.4, 35°C 717431 3.4.21.9 0.525 - glycyl-tetra-L-aspartyl-L-lysine beta-naphthylamide pH 8.0, 37°C 667759 3.4.21.9 0.6 - GDDDDK-2-naphthylamide mutant enzyme E136Y, at pH 8.3 and 30°C 732855 3.4.21.9 0.6 - GDDDDK-2-naphthylamide wild type enzyme, at pH 8.3 and 30°C 732855 3.4.21.9 0.61 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide - 95577 3.4.21.9 0.66 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide mutant enzyme K97A 95577 3.4.21.9 0.7 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide 37°C, pH 7.4 716743 3.4.21.9 0.7 - t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin mutant enzyme H24Q, at pH 8.3 and 30°C 732855 3.4.21.9 0.72 - Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly - 696841 3.4.21.9 0.75 - Gly-(L-Asp)4-L-Lys-2-naphthylamide pH and temperature not specified in the publication 732803 3.4.21.9 0.76 - Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly - 696841 3.4.21.9 0.77 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide mutant enzyme K98A 95577 3.4.21.9 0.8 - t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin mutant enzyme R213L, at pH 8.3 and 30°C 732855 3.4.21.9 0.8 - Val-(Asp)3-Glu-Lys-Ile-Val-Gly - 696841 3.4.21.9 0.9 - benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme R213L, at pH 8.3 and 30°C 732855 3.4.21.9 0.907 - APFDDDDKIVGG at 0.08-2 mM, holoenzyme 684036 3.4.21.9 1 - Boc-Glu(OBzl)-Ala-Arg-MCA E173A mutant, 37°C, pH 7.4 716743 3.4.21.9 1 - Boc-Glu(OBzl)-Ala-Arg-MCA P193E mutant, 37°C, pH 7.4 716743 3.4.21.9 1 - Pro-Phe-Arg-4-methylcoumaryl-7-amide E173A mutant, 37°C, pH 7.4 716743 3.4.21.9 1 - Pro-Phe-Arg-4-methylcoumaryl-7-amide P193E mutant, 37°C, pH 7.4 716743 3.4.21.9 1.01 - Val-Ala-(Asp)3-Lys-Ile-Val-Gly - 696841 3.4.21.9 1.06 - APFDDDDKIVGG at 0.1-3 mM, holoenzyme 684036 3.4.21.9 1.1 - benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme H24Q, at pH 8.3 and 30°C 732855 3.4.21.9 1.1 - Pro-Phe-Arg-4-methylcoumaryl-7-amide K63R mutant, 37°C, pH 7.4 716743 3.4.21.9 1.12 - Gly-Asp-ASp-Asp-Asp-2-naphthylamide - 95584 3.4.21.9 1.2 - Boc-Glu(OBzl)-Ala-Arg-MCA K63R mutant, 37°C, pH 7.4 716743 3.4.21.9 1.2 - bovine trypsinogen 37 °C, 25 mM Tris-HCl pH 8.4, 10 mM CaCl2, 0. 4 mM ovomucoid, 0.3 nM enteropeptidase 669370 3.4.21.9 1.2 - Trypsinogen pH 8.4 95578 3.4.21.9 1.24 - benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide - 95584 3.4.21.9 1.24 - Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly - 696841 3.4.21.9 1.25 - Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide mutant enzyme K96A 95577 3.4.21.9 1.25 - Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly - 696841 3.4.21.9 1.3 - Boc-Glu(OBzl)-Ala-Arg-MCA T105E mutant, 37°C, pH 7.4 716743 3.4.21.9 1.3 - Pro-Phe-Arg-4-methylcoumaryl-7-amide T105E mutant, 37°C, pH 7.4 716743 3.4.21.9 1.3 - t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin mutant enzyme E136Y, at pH 8.3 and 30°C 732855 3.4.21.9 1.41 - APFDDDDKIVGG at 0.03-0.08 mM, EP light chain 684036 3.4.21.9 1.5 - human cationic trypsinogen 37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.45 nM enteropeptidase 669370 3.4.21.9 1.5 - t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin wild type enzyme, at pH 8.3 and 30°C 732855 3.4.21.9 1.51 - Val-(Ala)3-Asp-Lys-Ile-Val-Gly - 696841 3.4.21.9 1.6 - benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme E136Y, at pH 8.3 and 30°C 732855 3.4.21.9 1.6 - benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin wild type enzyme, at pH 8.3 and 30°C 732855 3.4.21.9 1.6 - WDDKG in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 1.77 - Val-(Asp)4-Lys-Ile-Val-Gly - 696841 3.4.21.9 2.1 - WDDRG in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 3.3 - angiotensin II in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 3.39 - Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly - 696841 3.4.21.9 4 - MLTAEEKAA in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 4.2 - LTAEEKA in 0.1 M Tris-HCl buffer pH 8.0 at 37°C 667758 3.4.21.9 5.57 - Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly - 696841 3.4.21.9 5.6 - bovine trypsinogen 21 °C, 50 mM sodium citrate pH 5.6, 1 nM enteropeptidase 669370 3.4.21.9 8.31 - Val-(Asp)3-Ala-Lys-Ile-Val-Gly - 696841 3.4.21.9 8.77 - Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly - 696841 3.4.21.9 10 - Pro-Phe-Arg-4-methylcoumaryl-7-amide 37°C, pH 7.4 716743 3.4.21.9 21.11 - Val-Asp-(Ala)3-Lys-Ile-Val-Gly - 696841 3.4.21.9 596 - N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + 37°C, pH 8.0 667759