3.4.21.50 0.0007 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S 651790 3.4.21.50 0.0013 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A 651790 3.4.21.50 0.0019 - Trypsinogen - 36606, 36618 3.4.21.50 0.0021 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme 651790 3.4.21.50 0.0028 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme 651790 3.4.21.50 0.0034 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L 651790 3.4.21.50 0.0036 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F 651790 3.4.21.50 0.0037 - tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme 651790 3.4.21.50 0.004 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F 651790 3.4.21.50 0.0043 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y 651790 3.4.21.50 0.0046 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L 651790 3.4.21.50 0.0058 - tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F 651790 3.4.21.50 0.006 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210S 651790 3.4.21.50 0.0061 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A 651790 3.4.21.50 0.0063 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y 651790 3.4.21.50 0.0072 - tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y 651790 3.4.21.50 0.0073 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A 651790 3.4.21.50 0.0099 - tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L 651790 3.4.21.50 0.01 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme H210A 651790 3.4.21.50 0.01 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V 651790 3.4.21.50 0.012 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V 651790 3.4.21.50 0.017 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H 651790 3.4.21.50 0.02 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A 651790 3.4.21.50 0.023 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A 651790 3.4.21.50 0.024 - tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H 651790 3.4.21.50 0.029 - tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169F 651790 3.4.21.50 0.032 - tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169G 651790 3.4.21.50 0.034 - N-tosyl-Gly-Pro-Lys-p-nitroanilide - 36616 3.4.21.50 0.034 - tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A 651790 3.4.21.50 0.034 - tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H 651790 3.4.21.50 0.034 - tosyl-Gly-Pro-Lys-p-nitroanilide pH 9.0 36616 3.4.21.50 0.037 - tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169Y 651790 3.4.21.50 0.038 - tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, wild-type enzyme 651790 3.4.21.50 0.042 - tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V 651790 3.4.21.50 0.046 - tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169L 651790 3.4.21.50 0.053 - N-tosyl-L-Lys methyl ester - 36616 3.4.21.50 0.053 - Tosyl-Lys methyl ester pH 8.0 36616 3.4.21.50 0.066 - tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169A 651790 3.4.21.50 0.07 - N-benzoyl-L-Lys p-nitroanilide - 36618 3.4.21.50 0.07 - N-benzoyl-L-Lys p-nitroanilide protease I 36604, 36609 3.4.21.50 0.074 - tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169H 651790 3.4.21.50 0.08 - Lys-p-nitroanilide - 36618 3.4.21.50 0.08 - Lys-p-nitroanilide protease I 36604, 36609 3.4.21.50 0.081 - tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin pH 9.0, 37°C, mutant enzyme W169V 651790 3.4.21.50 0.083 - N-tosyl-L-Lys methyl ester protease Ia 36604 3.4.21.50 0.09 - N-benzoyl-L-Lys p-nitroanilide protease Ia 36604 3.4.21.50 0.091 - benzoyl-Lys methyl ester - 36607, 36618 3.4.21.50 0.1 - N-tosyl-L-Lys methyl ester - 36618 3.4.21.50 0.1 - N-tosyl-L-Lys methyl ester protease I 36604, 36607 3.4.21.50 0.11 - Lys-p-nitroanilide protease Ia 36604 3.4.21.50 0.25 - tosyl-Gly-Pro-Lys-p-nitroanilide - 36614 3.4.21.50 0.32 - benzoyl-Lys-NH2 - 36609, 36618 3.4.21.50 0.33 - Val-Leu-Lys-p-nitroanilide - 36614 3.4.21.50 0.63 - N-benzoyl-L-Orn methyl ester protease Ia 36604 3.4.21.50 1 - N-benzoyl-L-Orn methyl ester - 36618 3.4.21.50 1 - N-benzoyl-L-Orn methyl ester protease I 36604, 36607 3.4.21.50 1.59 - N-acetyl-L-lysine pH 9, rate of carboxyl oxygen exchange reaction by Lys-C is accelerated at acidic pH conditions. 683817 3.4.21.50 2.5 - N-tosyl-L-Arg methyl ester protease Ia 36604 3.4.21.50 3.09 - N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide - 36618 3.4.21.50 3.11 - N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide - 36618 3.4.21.50 20 - benzoyl-Arg ethyl ester - 36607, 36609 3.4.21.50 35.7 - N-tosyl-L-Arg methyl ester - 36618 3.4.21.50 35.7 - N-tosyl-L-Arg methyl ester protease I 36604, 36607, 36609 3.4.21.50 41 - N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide - 36618