3.4.21.27 additional information - additional information kinetics of isolated subunits with each one active site, stoichiometry of FIX and FIXalphabeta binding to FXIa, overview 683682 3.4.21.27 additional information - additional information kinetics of recombinant wild-type and mutant enzymes, overview 683169 3.4.21.27 0.000103 - prochemerin chem163S at pH 7.4 and 37°C 753069 3.4.21.27 0.00012 - factor IX FXIa/PKA4, 37°C 650724 3.4.21.27 0.00016 - factor IX FXIa/PKA4-Gly326, 37°C 650724 3.4.21.27 0.0002 - factor IX factor FXIa, 37°C 650724 3.4.21.27 0.0002 0.00021 factor IX 37°C, recombinant wild-type enzyme 683167 3.4.21.27 0.00025 - factor IX at 25°C, pH not specified in the publication 752757 3.4.21.27 0.0003 - factor IX wild type enzyme, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.00035 - factor IX mutant G317R at 25°C, pH not specified in the publication 752757 3.4.21.27 0.00038 - factor IX mutant G317E at 25°C, pH not specified in the publication 752757 3.4.21.27 0.00039 0.00047 factor IX 37°C, recombinant peptide FXI/E361-V607 683167 3.4.21.27 0.053 - factor IX pH 7.4, 37°C, recombinant wild-type enzyme 683682 3.4.21.27 0.09 - benzyloxycarbonyl-Gly-Pro-4-nitroanilide - 36541 3.4.21.27 0.09 - factor fIX FXIa 669347 3.4.21.27 0.151 - factor IX at 22°C 667583 3.4.21.27 0.205 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme K192R, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.225 - S-2366 37°C, recombinant wild-type enzyme 683167 3.4.21.27 0.26 - pyroGlu-Pro-Arg-4-nitroanilide recombinant wild-type enzyme, pH 7.5, 37°C 683169 3.4.21.27 0.268 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme R3704A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.292 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme Y5901A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.3 - factor IX 10-mer at pH 7.4 and 37°C 753069 3.4.21.27 0.315 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme K192E, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.336 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme Y5901V, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.339 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide wild type enzyme, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.34 - D-Ile-Pro-Arg-4-nitroanilide recombinant wild-type enzyme, pH 7.5, 37°C 683169 3.4.21.27 0.357 - S-2366 37°C, recombinant peptide FXI/E361-V607 683167 3.4.21.27 0.357 - S-2366 FXIa 667620 3.4.21.27 0.383 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme E98D, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.384 - S-2366 mutant FXIa/G326C 667620 3.4.21.27 0.4 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme E98A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.4 - L-pyroglutamyl-L-prolyl-L-arginyl-p-nitroanilide - 667611 3.4.21.27 0.4 - pyroGlu-Pro-Arg-4-nitroanilide pH 7.4, 37°C, recombinant wild-type enzyme 683682 3.4.21.27 0.45 - L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide FXIa/PKA4 650724 3.4.21.27 0.5 - L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide factor FXIa 650724 3.4.21.27 0.525 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme E98V, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.538 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme Y143A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.552 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-K170A/R171A/R173A mutant 691011 3.4.21.27 0.56 - pyroGlu-Pro-Arg-p-nitroanilide - 36532 3.4.21.27 0.59 - L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide FXIa/PKA4-Gly326 650724 3.4.21.27 0.603 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme K192Q, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.655 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-K170A mutant 691011 3.4.21.27 0.757 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-wild type 691011 3.4.21.27 0.764 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-K179A mutant 691011 3.4.21.27 0.81 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-R173A mutant 691011 3.4.21.27 0.812 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-R171A mutant 691011 3.4.21.27 0.828 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme I151A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.829 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-K175A mutant 691011 3.4.21.27 0.852 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme K192A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.943 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-wild type 691011 3.4.21.27 0.993 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-R37Q mutant 691011 3.4.21.27 233 - L-pyroglutamyl-L-prolyl-L-arginyl-p-nitroanilide FXIa 669347 3.4.21.27 352 - L-pyroglutamyl-L-prolyl-L-arginyl-p-nitroanilide fXIaCD 669347