3.2.2.16 additional information - additional information kinetics and thermodynamics, overview 709718 3.2.2.16 additional information - additional information Michaelis-Menten kinetics 728199 3.2.2.16 additional information - additional information Michelis-Menten kinetics, substrate 5'-deoxyadenosine 708504 3.2.2.16 0.00000019 - S-methyl-5'-thioadenosine in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 731300 3.2.2.16 0.00000036 - S-methyl-5'-thioadenosine in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 731300 3.2.2.16 0.0000004 - S-methyl-5'-thioadenosine in 100 mM HEPES, 50 mM KCl (pH 7.5), at 25°C 731300 3.2.2.16 0.000061 - S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C 727044 3.2.2.16 0.000139 - S-methyl-5'-thioadenosine wild type enzyme, at pH 7.7 and 25°C 727044 3.2.2.16 0.00019 - 5'-methylthioadenosine pH 7.5, 25°C, recombinant enzyme 731300 3.2.2.16 0.000202 - S-methyl-5'-thioadenosine mutant enzyme A113P/V153I/R158G, at pH 7.7 and 25°C 727044 3.2.2.16 0.000262 - S-methyl-5'-thioadenosine mutant enzyme A113P, at pH 7.7 and 25°C 727044 3.2.2.16 0.000262 - S-methyl-5'-thioadenosine mutant enzyme A113P/V153I, at pH 7.7 and 25°C 727044 3.2.2.16 0.00031 - 5'-deoxy-5'-methylthioadenosine - 171783, 171784 3.2.2.16 0.00036 - 5'-methylthioadenosine pH 7.5, 25°C, recombinant enzyme 731300 3.2.2.16 0.0004 - 5'-methylthioadenosine in 50 mM potassium phosphate buffer, pH 7.0, at 37°C 171854 3.2.2.16 0.0004 - 5'-methylthioadenosine pH 7.5, 25°C, recombinant enzyme 731300 3.2.2.16 0.00041 - 5'-deoxy-5'-methylthioadenosine - 171783, 171784 3.2.2.16 0.00043 - S-methyl-5'-thioadenosine mutant enzyme V153I, at pH 7.7 and 25°C 727044 3.2.2.16 0.0005 - 5'-methylthioadenosine - 171791 3.2.2.16 0.00055 - 5'-methylthioinosine - 171783 3.2.2.16 0.0007 - 5'-methylthioadenosine Pfs protein, at pH 5.0 698517 3.2.2.16 0.0009 - S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C 727044 3.2.2.16 0.00124 - 5'-methylthioadenosine recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 695346 3.2.2.16 0.0022 - S-methyl-5'-thioadenosine wild type enzyme, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0029 - S-methyl-5'-thioadenosine mutant enzyme F104C/C186S, at pH 7.2 and 25°C 727044 3.2.2.16 0.003 - 5'-methylthioadenosine at 25°C, in 100 mM HEPES, pH 7.5, and 50 mM KCl 700355 3.2.2.16 0.0031 - S-methyl-5'-thioadenosine mutant enzyme A15M, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0034 - 5'-methylthioadenosine 22°C 681477 3.2.2.16 0.004 - S-adenosyl-L-homocysteine wild type enzyme, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0041 - S-adenosyl-L-homocysteine mutant enzyme A15M, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0044 - S-methyl-5'-thioadenosine mutant enzyme S167A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0045 - S-methyl-5'-thioadenosine mutant enzyme V34I, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0048 - S-methyl-5'-thioadenosine mutant enzyme R85H, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.005 - 5'-methylthioadenosine Bgp protein, at pH 5.0 698517 3.2.2.16 0.0053 - S-methyl-5'-thioadenosine mutant enzyme V34A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0054 - S-methyl-5'-thioadenosine mutant enzyme R85A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0054 - S-methyl-5'-thioadenosine mutant enzyme W179A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0056 - S-adenosyl-L-homocysteine mutant enzyme S167A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0058 - S-adenosyl-L-homocysteine mutant enzyme V34I, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0061 - S-adenosyl-L-homocysteine mutant enzyme R85H, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0062 - S-adenosyl-L-homocysteine mutant enzyme V34A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0068 - S-adenosyl-L-homocysteine mutant enzyme R85A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0071 - 5'-methylthioadenosine 22°C 681477 3.2.2.16 0.0071 - 5'-methylthioadenosine in 100 mM sodium phosphate buffer, pH 7.0 681477 3.2.2.16 0.00725 - 5'-deoxy-5'-methylthioadenosine - 171785 3.2.2.16 0.00738 - 5'-methylthioadenosine recombinant enzyme, in 50 mM potassium phosphate, pH 7.0 695346 3.2.2.16 0.0074 - S-methyl-5'-thioadenosine mutant enzyme W179F, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0076 - S-adenosyl-L-homocysteine mutant enzyme W179A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.008 - S-methyl-5'-thioadenosine mutant enzyme R164A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0098 - S-adenosyl-L-homocysteine mutant enzyme W179F, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.01 - S-adenosyl-L-homocysteine wild type enzyme, at pH 7.2 and 25°C 727008 3.2.2.16 0.0103 - 5'-deoxy-5'-methylthioadenosine - 171788 3.2.2.16 0.013 - S-adenosyl-L-homocysteine pH 7.5, 25°C 678158 3.2.2.16 0.0133 - S-adenosyl-L-homocysteine mutant enzyme R164A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.014 - S-adenosyl-L-homocysteine mutant enzyme H109A, at pH 7.2 and 25°C 727008 3.2.2.16 0.015 - 5'-deoxy-5'-methylthioadenosine - 171782 3.2.2.16 0.019 - S-adenosyl-L-homocysteine mutant enzyme F107A, at pH 7.2 and 25°C 727008 3.2.2.16 0.019 - S-methyl-5'-thioadenosine at pH 7.5 and 25°C 727555 3.2.2.16 0.023 - 5'-methylthioadenosine pH 7.5, 25°C 678158 3.2.2.16 0.028 - S-methyl-5'-thioadenosine mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.0301 - S-methyl-5'-thioadenosine mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.033 - S-methyl-5'-thioadenosine mutant enzyme H109A, at pH 7.2 and 25°C 727008 3.2.2.16 0.0351 - S-methyl-5'-thioadenosine mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.037 - S-methyl-5'-thioadenosine mutant enzyme F107A, at pH 7.2 and 25°C 727008 3.2.2.16 0.0381 - S-adenosyl-L-homocysteine mutant enzyme Y134A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.039 - S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C 727008 3.2.2.16 0.0435 - 5'-deoxy-5'-methylthioadenosine - 171786 3.2.2.16 0.0452 - S-adenosyl-L-homocysteine mutant enzyme Y134F, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.055 - 5'-methylthioinosine - 171784 3.2.2.16 0.0951 - S-adenosyl-L-homocysteine mutant enzyme M144A, in 100 mM HEPES (pH 7.5), at 25°C 726889 3.2.2.16 0.43 - 5'-methylthioadenosine pH 7.5, 25°C 678158 3.2.2.16 1.3 - S-adenosyl-L-homocysteine pH 7.5, 25°C 678158 3.2.2.16 2.1 - 5'-methylthioadenosine pH 7.0, 22°C 681132