2.7.7.23 additional information - additional information kinetics of wild-type and mutant enzymes for Glc-1-P UTase activities, overview 755852 2.7.7.23 additional information - additional information Km values are 3.54 microM/l, 13.9 microM/l, 4.22 microM/l, 13.3 microM/l for isoforms MP I, MP II, MP III, MP IV, respcetively 722855 2.7.7.23 additional information - additional information Michaelis-Menten kinetics 737291, 741091 2.7.7.23 additional information - additional information the UAP enzyme kinetics display a strictly ordered bi.bi mechanism 737319 2.7.7.23 0.0017 - UTP pH 7.5, 80°C 725308, 748123 2.7.7.23 0.008 - N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 80°C 725308, 748123 2.7.7.23 0.008 - UTP pH 7.5, 30°C 756049 2.7.7.23 0.011 - N-acetyl-D-glucosamine 1-phosphate R116A mutant, 30°C, pH 8.3 643068 2.7.7.23 0.012 - N-acetyl-D-glucosamine 1-phosphate P122A mutant, 30°C, pH 8.3 643068 2.7.7.23 0.014 - N-acetyl-D-glucosamine 1-phosphate wild type enzyme, 30°C, pH 8.3 643068 2.7.7.23 0.015 - N-acetyl-D-glucosamine 1-phosphate T115A mutant, 30°C, pH 8.3 643068 2.7.7.23 0.016 - diphosphate pH 7.5, 80°C 725308, 748123 2.7.7.23 0.016 - UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 80°C 725308, 748123 2.7.7.23 0.018 - N-acetyl-D-glucosamine 1-phosphate K123A mutant, 30°C, pH 8.3 643068 2.7.7.23 0.019 - N-acetyl-D-glucosamine 1-phosphate G114A mutant, 30°C, pH 8.3 643068 2.7.7.23 0.024 - UTP pH 7.5, 37°C 718708 2.7.7.23 0.029 - N-acetyl-D-glucosamine 1-phosphate L117A mutant, 30°C, pH 8.3 643068 2.7.7.23 0.03 - N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 30°C 756049 2.7.7.23 0.03 - N-acetyl-alpha-D-glucosamine 1-phosphate purified recombinant native enzyme, pH 7.5, 37°C 662429 2.7.7.23 0.03 - UTP pH 7.5, 37°C, recombinant enzyme 737291 2.7.7.23 0.034 - N-acetyl-D-glucosamine 1-phosphate G111A mutant, 30°C, pH 8.3 643068 2.7.7.23 0.038 - UTP Mg-UTP, purified recombinant native enzyme, pH 7.5, 37°C 662429 2.7.7.23 0.041 - N-acetyl-alpha-D-glucosamine 1-phosphate pH 7.5, 37°C, recombinant enzyme 737291 2.7.7.23 0.044 - N-acetyl-D-glucosamine 1-phosphate pH 7.5, 37°C 718708 2.7.7.23 0.061 - N-acetyl-alpha-D-glucosamine 1-phosphate pH 8.2, 37°C, recombinant His6-tagged enzyme 735569 2.7.7.23 0.065 - UDP-N-acetyl-D-glucosamine pH 7.6, 37°C 721526 2.7.7.23 0.068 - N-acetyl-alpha-D-glucosamine 1-phosphate wild-type enzyme, pH 7.5, 80°C 746841, 755852 2.7.7.23 0.068 - N-acetyl-alpha-D-glucosamine 1-phosphate wild-type ST0452, pH 7.5, 80°C 755852 2.7.7.23 0.07 - N-acetyl-D-glucosamine 1-phosphate pH 8.2, 37°C 288685 2.7.7.23 0.095 - UTP pH 8.2, 37°C, recombinant His6-tagged enzyme 735569 2.7.7.23 0.097 - N-acetyl-alpha-D-glucosamine 1-phosphate mutant enzyme Y97M, pH 7.5, 80°C 746841, 755852 2.7.7.23 0.097 - N-acetyl-alpha-D-glucosamine 1-phosphate mutant Y97M, pH 7.5, 80°C 755852 2.7.7.23 0.1 - UTP pH 8.2, 37°C 288685 2.7.7.23 0.124 - N-acetyl-D-glucosamine 1-phosphate G112A mutant, 30°C, pH 8.3 643068 2.7.7.23 0.147 - N-acetyl-alpha-D-glucosamine 1-phosphate mutant enzyme Y97N, pH 7.5, 80°C 746841, 755852 2.7.7.23 0.147 - N-acetyl-alpha-D-glucosamine 1-phosphate mutant Y97N, pH 7.5, 80°C 755852 2.7.7.23 0.18 - N-acetyl-D-glucosamine 1-phosphate pH 7.6, 37°C 721526 2.7.7.23 0.203 - UTP pH 7.6, 37°C 721526 2.7.7.23 0.219 - UDP-N-acetyl-D-glucosamine pH 7.6, 37°C 721526 2.7.7.23 0.23 - UDP-N-acetyl-D-glucosamine pH 8.5, 37°C, 5 mM diphosphate 643065 2.7.7.23 0.29 - N-acetyl-D-glucosamine 1-phosphate pH 7.5, 37°C, 5 mM UTP 643065 2.7.7.23 0.296 - UTP pH 7.6, 37°C 721526 2.7.7.23 0.337 - N-acetyl-D-glucosamine 1-phosphate pH 7.6, 37°C 721526 2.7.7.23 0.36 - UDP-N-acetyl-D-glucosamine pH 7.4, 37°C 643057 2.7.7.23 0.38 - N-acetyl-alpha-D-galactosamine 1-phosphate AGX1, 37°C, pH 7.5 643069 2.7.7.23 0.4 - UDP-N-acetyl-D-glucosamine 30°C 643067 2.7.7.23 0.42 - UTP pH 7.5, 37°C, 10 mM N-acetyl-D-glucose 1-phosphate 643065 2.7.7.23 0.57 - diphosphate 30°C 643067 2.7.7.23 0.7 - UDP-N-acetyl-D-glucosamine AGX1, pH 8.5, 37°C 643070 2.7.7.23 0.79 - diphosphate pH 7.4, 37°C 643057 2.7.7.23 0.8 - UDP-N-acetyl-D-glucosamine P220A, Y227A, R115A mutants, pH 8.5, 37°C 643070 2.7.7.23 0.808 - UDP-N-acetyl-D-galactosamine pH 7.6, 37°C 721526 2.7.7.23 0.861 - N-acetyl-alpha-D-glucosamine 1-phosphate mutant enzyme Y97V, pH 7.5, 80°C 746841, 755852 2.7.7.23 0.861 - N-acetyl-alpha-D-glucosamine 1-phosphate mutant Y97V, pH 7.5, 80°C 755852 2.7.7.23 1.1 - N-acetyl-alpha-D-galactosamine 1-phosphate pH 7.5, 37°C, 5 mM UTP 643065 2.7.7.23 1.21 - UTP 30°C 643067 2.7.7.23 1.3 - N-acetyl-alpha-D-galactosamine 1-phosphate AGX2, 37°C, pH 7.5 643069 2.7.7.23 2.2 - UDP-N-acetyl-D-galactosamine pH 8.5, 37°C, 5 mM diphosphate 643065 2.7.7.23 2.2 - UDP-N-acetyl-D-glucosamine G224A mutants, pH 8.5, 37°C 643070 2.7.7.23 2.2 - UDP-N-acetyl-D-glucosamine pH 7.5, 30°C 643061 2.7.7.23 2.25 - N-acetyl-D-glucosamine 1-phosphate 30°C 643067 2.7.7.23 2.768 - UDP-N-acetyl-D-galactosamine pH 7.6, 37°C 721526 2.7.7.23 5 - diphosphate pH 7.5, 30°C 643059 2.7.7.23 5.4 - diphosphate pH 7.5, 30°C 643061 2.7.7.23 5.8 - D-glucose 1-phosphate pH 7.5, 37°C, 5 mM UTP 643065 2.7.7.23 6.1 - UDP-N-acetyl-D-glucosamine pH 7.5, 30°C 643059 2.7.7.23 6.3 - UDP-D-glucose pH 8.5, 37°C, 5 mM diphosphate 643065