2.7.4.14 additional information - additional information Km values of mutant enzymes, overview 692040 2.7.4.14 0.0053 - CMP reaction with ATP, enzyme from Novikoff ascites hepatoma 645139 2.7.4.14 0.0146 - gemcitabine wild-type DCK 692040 2.7.4.14 0.015 - CMP 37°C, 196-aa and 228-aa enzyme form, in presence of 8 mM MgATP2- 645161 2.7.4.14 0.017 - dCMP pH 8.0, 37°C 645155 2.7.4.14 0.02 - CMP pH and temperature not specified in the publication, recombinant wild-type enzyme 739215 2.7.4.14 0.02 - D-CMP - 676227 2.7.4.14 0.02 - D-CMP pH 7.4, 37°C 694479 2.7.4.14 0.02 - UMP pH 7.4, 37°C, His-tagged UMP/CMP kinase 645162 2.7.4.14 0.022 - CMP pH 7.4, 37°C 645162 2.7.4.14 0.023 - CMP pH 8.0, 37°C 645155 2.7.4.14 0.023 - dCMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- 645161 2.7.4.14 0.026 - CMP 37°C enzyme form UMPK 1 645146 2.7.4.14 0.027 - dCMP - 645143 2.7.4.14 0.027 - dCMP reaction with dATP, liver enzyme 645139 2.7.4.14 0.028 - CMP 37°C, enzyme form UMPK2 645146 2.7.4.14 0.029 - ATP pH 6.5, when UMP is the other substrate 645153 2.7.4.14 0.03 - CMP - 645143 2.7.4.14 0.03 - CMP reaction with ATP, liver enzyme 645139 2.7.4.14 0.04 - UMP - 645143 2.7.4.14 0.04 - UMP reaction with ATP, liver enzyme 645139 2.7.4.14 0.043 - UMP reaction with ATP, enzyme from Novikoff ascites hepatoma 645139 2.7.4.14 0.044 - UMP pH 7.4, 37°C 645162 2.7.4.14 0.045 - UMP pH 7.4, 37°C, His-tagged UMP/CMP kinase 645162 2.7.4.14 0.05 - D-UMP - 676227 2.7.4.14 0.05 - D-UMP pH 7.4, 37°C 694479 2.7.4.14 0.052 - UMP pH 7.5, 25°C 645149 2.7.4.14 0.053 - UMP - 645143 2.7.4.14 0.053 - UMP 37°C, enzyme form UMPK1 645146 2.7.4.14 0.053 - UMP reaction with dATP, liver enzyme 645139 2.7.4.14 0.064 - UMP 37°C, enzyme form UMPK2 645146 2.7.4.14 0.067 - ATP pH 7.1, 37°C 645150 2.7.4.14 0.067 - UMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- 645161 2.7.4.14 0.069 - UMP 37°C, 228-aa enzyme form, in presence of 8 mM MgATP2- 645161 2.7.4.14 0.07 - CMP - 673598 2.7.4.14 0.07 - CMP pH 7.4, 37°C, GST fusion UMP-CMP kinase 645162 2.7.4.14 0.071 - CMP pH 7.5, 25°C 645149 2.7.4.14 0.074 - dATP reaction with CMP, liver enzyme 645139 2.7.4.14 0.095 - UMP pH 8.0, 37°C 645155 2.7.4.14 0.1 - dUMP pH 8.0, 37°C, wild-type enzyme 693063 2.7.4.14 0.1 - UMP pH and temperature not specified in the publication, recombinant wild-type enzyme 739215 2.7.4.14 0.11 - dCMP pH and temperature not specified in the publication, recombinant wild-type enzyme 739215 2.7.4.14 0.123 - CMP pH and temperature not specified in the publication, recombinant mutant P64T 739215 2.7.4.14 0.13 - CMP pH 7.1, 37°C 645150 2.7.4.14 0.13 - UMP - 673598 2.7.4.14 0.134 - ATP reaction with dCMP, enzyme from Novikoff ascites hepatoma 645139 2.7.4.14 0.14 - UMP pH 7.4, 37°C, GST fusion UMP-CMP kinase 645162 2.7.4.14 0.15 - beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C, His-tagged UMP/CMP kinase 645162 2.7.4.14 0.15 - L-3TCMP - 676227 2.7.4.14 0.153 - UMP pH 6.5 645153 2.7.4.14 0.2 - CMP pH 7.4, 37°C 645162 2.7.4.14 0.2 - UMP pH 7.4, 37°C 645162 2.7.4.14 0.204 - ATP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- 645161 2.7.4.14 0.211 - CMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- 645161 2.7.4.14 0.228 - beta-L-(-)-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP 37°C 645161 2.7.4.14 0.246 - gemcitabine phosphate wild-type CMPK 692040 2.7.4.14 0.25 - ATP 37°C, enzyme form UMPK2 645146 2.7.4.14 0.25 - L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP 37°C, His-tagged UMP/CMP kinase 645161 2.7.4.14 0.25 - UMP pH and temperature not specified in the publication, recombinant mutant P64T 739215 2.7.4.14 0.26 - ara-CMP pH 7.4, 37°C, His-tagged UMP/CMP kinase 645162 2.7.4.14 0.266 - CMP pH 6.5 645153 2.7.4.14 0.272 - beta-D-2',3'-dideoxy-CMP 37°C 645161 2.7.4.14 0.292 - ATP pH 6.5, reaction with CMP 645153 2.7.4.14 0.3 - beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C, GST fusion UMP-CMP kinase 645162 2.7.4.14 0.315 - cytarabine pH and temperature not specified in the publication, recombinant wild-type enzyme 739215 2.7.4.14 0.32 - ATP reaction with CMP, liver enzyme 645139 2.7.4.14 0.327 - cytarabine pH and temperature not specified in the publication, recombinant mutant P64T 739215 2.7.4.14 0.34 - ara-CMP pH 7.4, 37°C, GST fusion UMP-CMP kinase 645162 2.7.4.14 0.35 - ATP pH 7.1, 37°C 645150 2.7.4.14 0.354 - dCMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- 645161 2.7.4.14 0.36 - 2'-azido-D-dCMP pH 7.4, 37°C 694479 2.7.4.14 0.36 - ATP 37°C, enzyme form UMPK1 645146 2.7.4.14 0.36 - beta-D-2'-azido-2'-dCMP - 676227 2.7.4.14 0.42 - dATP reaction with UMP, liver enzyme 645139 2.7.4.14 0.43 - dUMP pH 8.0, 37°C 645155 2.7.4.14 0.45 - 2',2'-difluorodeoxycytidine pH 8.0, 37°C 645156 2.7.4.14 0.494 - L-(-)-2',3'-dideoxy-3'-thia-CMP 37°C 645161 2.7.4.14 0.5 - ATP - 645140 2.7.4.14 0.5 - CMP pH 8.0, 37°C 645156 2.7.4.14 0.513 - dCMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- 645161 2.7.4.14 0.54 - dCMP pH 7.5, 25°C 645149 2.7.4.14 0.58 - ATP reaction with UMP, liver enzyme 645139 2.7.4.14 0.581 - gemcitabine monophosphate 37°C 645161 2.7.4.14 0.61 - dATP reaction with dCMP, liver enzyme 645139 2.7.4.14 0.68 - ATP reaction with dCMP, liver enzyme 645139 2.7.4.14 0.697 - beta-L-2',3'-dideoxy-CMP 37°C 645161 2.7.4.14 0.7 - L-dUMP L-enantiomers have lower reaction rates 676227 2.7.4.14 0.7 - L-dUMP pH 7.4, 37°C 694479 2.7.4.14 0.715 - dCMP reaction with ATP, enzyme from Novikoff ascites hepatoma 645139 2.7.4.14 0.73 - L-dCMP L-enantiomers have lower reaction rates 676227 2.7.4.14 0.73 - L-dCMP pH 7.4, 37°C 694479 2.7.4.14 0.75 - L-CMP - 676227 2.7.4.14 0.75 - L-CMP pH 7.4, 37°C 694479 2.7.4.14 0.8 - CMP - 645140 2.7.4.14 0.82 - dCTP reaction with CMP, liver enzyme 645139 2.7.4.14 0.9 - 2'-azido-D-dUMP pH 7.4, 37°C 694479 2.7.4.14 0.9 - beta-D-2'-azido-2'-dUMP - 676227 2.7.4.14 0.9 - dCMP pH 7.4, 37°C, His-tagged UMP/CMP kinase 645162 2.7.4.14 0.917 - ara-CMP 37°C 645161 2.7.4.14 0.97 - dCMP pH and temperature not specified in the publication, recombinant mutant P64T 739215 2.7.4.14 0.98 - CMP - 645143 2.7.4.14 0.98 - CMP reaction with dCTP, liver enzyme 645139 2.7.4.14 1 - Cidofovir - 673598 2.7.4.14 1 - D-dCMP - 676227 2.7.4.14 1 - D-dCMP pH 7.4, 37°C 694479 2.7.4.14 1.034 - dUMP pH and temperature not specified in the publication, recombinant wild-type enzyme 739215 2.7.4.14 1.037 - beta-D-2',3'-dideoxy-CMP 37°C 645161 2.7.4.14 1.1 - dCMP - 673598 2.7.4.14 1.1 - dCMP pH 7.4, 37°C, GST fusion UMP-CMP kinase 645162 2.7.4.14 1.1 - dCMP reaction with dATP, liver enzyme 645139 2.7.4.14 1.2 - 2'-azido-L-dCMP pH 7.4, 37°C 694479 2.7.4.14 1.2 - beta-L-2'-azido-2'-dCMP - 676227 2.7.4.14 1.25 - CMP - 645140 2.7.4.14 1.3 - 2'-azido-L-dUMP pH 7.4, 37°C 694479 2.7.4.14 1.3 - beta-L-2'-azido-2'-dUMP - 676227 2.7.4.14 1.3 - D-dUMP - 676227 2.7.4.14 1.3 - D-dUMP pH 7.4, 37°C 694479 2.7.4.14 1.3 - dUMP - 673598 2.7.4.14 1.31 - dCMP pH 8.0, 37°C, wild-type enzyme 693063 2.7.4.14 1.4 - araCMP pH 8.0, 37°C 645156 2.7.4.14 1.4 - UMP pH 7.1, 37°C 645150 2.7.4.14 1.6 - UMP pH 8.0, 37°C 645156 2.7.4.14 2 - beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate pH 7.4, 37°C 645162 2.7.4.14 2 - cCMP pH 7.4, 37°C 645162 2.7.4.14 2.08 - 5-aza-cidofovir pH and temperature not specified in the publication, recombinant wild-type enzyme 739215 2.7.4.14 2.3 - Cidofovir pH and temperature not specified in the publication, recombinant wild-type enzyme 739215 2.7.4.14 2.77 - dCMP - 645143 2.7.4.14 2.77 - dCMP reaction with ATP, liver enzyme 645139 2.7.4.14 3.09 - CMP pH 8.0, 37°C, wild-type enzyme 693063 2.7.4.14 5.9 - dUMP pH 8.0, 37°C 645156 2.7.4.14 6.3 - UMP pH 8.0, 37°C, wild-type enzyme 693063 2.7.4.14 8.5 - dUMP pH 7.5, 25°C 645149