2.7.1.49 additional information - additional information - 662007 2.7.1.49 additional information - additional information kinetic analysis, Michaelis-Menten model, ordered sequential kinetic mechanism 758702 2.7.1.49 0.00104 - 4-Amino-5-hydroxymethyl-2-methylpyrimidine pH 7.5, temperature not specified in the publication, recombinant enzyme ThiD 758754 2.7.1.49 0.01 - 4-Amino-5-hydroxymethyl-2-methylpyrimidine pH 8.0, 25°C 758702 2.7.1.49 0.012 - 2-methyl-4-amino-5-hydroxymethylpyrimidine apparent value, in 100 mM triethanolamine or 50 mM potassium phosphate buffer, pH 7.5, 1 mM MgCl2, 500 mM ATP and 100 mM substrate 672477 2.7.1.49 0.017 - 4-Amino-5-hydroxymethyl-2-methylpyrimidine pH 8.0, 25°C 758702 2.7.1.49 0.0285 - pyridoxine pH 8.0, 25°C 641660 2.7.1.49 0.0335 - 2-methyl-4-amino-5-hydroxymethylpyrimidine pH 8.0, 25°C 641660 2.7.1.49 0.066 - pyridoxine pH 6.2, 37°C 641658 2.7.1.49 0.11 - 2-methyl-4-amino-5-hydroxymethylpyrimidine pH 6.2, 37°C 641658 2.7.1.49 0.111 - pyridoxal wild-type, pH not specified in the publication, temperature not specified in the publication 738451 2.7.1.49 0.117 - ATP apparent value, in 100 mM triethanolamine or 50 mM potassium phosphate buffer, pH 7.5, 1 mM MgCl2, 500 mM ATP and 100 mM substrate 672477 2.7.1.49 0.1431 - ATP pH 8.0, 25°C 641660 2.7.1.49 0.24 - pyridoxal pH 6.2, 37°C 641658 2.7.1.49 0.26 - pyridoxal mutant C214D, pH not specified in the publication, temperature not specified in the publication 738451 2.7.1.49 0.7 - ATP pH 8.0, 25°C 758702 2.7.1.49 0.74 - ATP pH 7.0, 25°C, recombinant enzyme, MgATP 758702 2.7.1.49 0.74 - ATP pH 8.0, 25°C 758702 2.7.1.49 1.51 - pyridoxine mutant C110A, pH not specified in the publication, temperature not specified in the publication 738451 2.7.1.49 1.85 - 4-Amino-5-hydroxymethyl-2-methylpyrimidine mutant C110A, pH not specified in the publication, temperature not specified in the publication 738451 2.7.1.49 1.99 - 4-Amino-5-hydroxymethyl-2-methylpyrimidine wild-type, pH not specified in the publication, temperature not specified in the publication 738451 2.7.1.49 2.07 - pyridoxine wild-type, pH not specified in the publication, temperature not specified in the publication 738451 2.7.1.49 2.4 - pyridoxamine pH 6.2, 37°C 641658 2.7.1.49 17 - 4-Amino-5-hydroxymethyl-2-methylpyrimidine pH 7.0, 25°C, recombinant enzyme 758702