2.7.1.19 additional information - additional information kinetics 641029 2.7.1.19 0.048 - D-ribulose 5-phosphate R186Q mutant, pH 8, 30°C 641052 2.7.1.19 0.05 0.08 D-ribulose 5-phosphate pH 7.8 and pH 6.8, 25°C 641020 2.7.1.19 0.056 - D-ribulose 5-phosphate pH 7.8, 25°C 641016 2.7.1.19 0.062 - ATP pH 7.8, 25°C 641016 2.7.1.19 0.07 - ATP pH 7.8 and pH 6.8, 25°C 641020 2.7.1.19 0.084 - ATP pH 8 663139 2.7.1.19 0.09 - ATP pH 8, 25°C 641051 2.7.1.19 0.096 - D-ribulose 5-phosphate wild-type enzyme, pH 8, 30°C 641049, 641050, 641052 2.7.1.19 0.1 - ATP - 641031 2.7.1.19 0.1 - D-ribulose 5-phosphate wild type enzyme, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate 677023 2.7.1.19 0.118 - D-ribulose 5-phosphate pH 8 663139 2.7.1.19 0.208 - ATP pH 8, 25°C 641045 2.7.1.19 0.222 - D-ribulose 5-phosphate pH 7.9, 20°C 641027 2.7.1.19 0.226 - D-ribulose 5-phosphate pH 8, 25°C 641045 2.7.1.19 0.24 - D-ribulose 5-phosphate 30°C 641030 2.7.1.19 0.27 - D-ribulose 5-phosphate pH 8, 25°C 641051 2.7.1.19 0.29 - D-ribulose 5-phosphate pH 7.5, 30°C 641017 2.7.1.19 0.29 0.33 D-ribulose 5-phosphate - 641031 2.7.1.19 0.55 - D-ribulose 5-phosphate R187Q mutant, pH 8, 30°C 641052 2.7.1.19 0.586 - D-ribulose 5-phosphate S14A mutant, pH 8, 30°C 641050 2.7.1.19 0.625 - ATP pH 7.9, 20°C 641027 2.7.1.19 0.662 - D-ribulose 5-phosphate T20A mutant, pH 8, 30°C 641050 2.7.1.19 0.71 - ATP 30°C 641030 2.7.1.19 0.78 - D-ribulose 5-phosphate mutant enzyme H100A, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate 677023 2.7.1.19 1.35 - D-ribulose 5-phosphate mutant enzyme H100Q, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate 677023 2.7.1.19 1.37 - D-ribulose 5-phosphate mutant enzyme H100N, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate 677023 2.7.1.19 1.42 - ATP pH 7.5, 30°C 641017 2.7.1.19 3.16 - D-ribulose 5-phosphate S19A mutant, pH 8, 30°C 641050 2.7.1.19 4 - D-ribulose 5-phosphate mutant enzyme Y98L, at 30°C, in 100 mM Tris-HCl (pH 8.2) containing 10 mM MgCl2, 0.5 mM dithiothreitol, 5 mM ATP, 0.2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 units of pyruvate kinase, 2 units of lactic dehydrogenase, and 1 mM D-ribulose 5-phosphate 677023 2.7.1.19 4.5 - D-ribulose 5-phosphate T18A mutant, pH 8, 30°C 641050 2.7.1.19 4.6 - D-ribulose 5-phosphate R168Q mutant, pH 8, 30°C 641052 2.7.1.19 10.3 - D-ribulose 5-phosphate K165M mutant, pH 8, 30°C 641049 2.7.1.19 10.5 - D-ribulose 5-phosphate R173Q mutant, pH 8, 30°C 641052 2.7.1.19 11 - D-ribulose 5-phosphate K165C mutant, pH 8, 30°C 641049