2.6.1.16 additional information - additional information binding energy calculations of wild-type and mutant enzymes 758961 2.6.1.16 additional information - additional information thermodynamics 760122 2.6.1.16 0.0064 - L-gamma-glutamyl-p-nitroanilide mutant W74F, 37°C 673687 2.6.1.16 0.04 - L-glutamine mutant enzyme W74L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.04 - L-glutamine mutant W74L, 37°C 673687 2.6.1.16 0.048 - L-glutamine mutant enzyme W74F, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.048 - L-glutamine mutant W74F, 37°C 673687 2.6.1.16 0.05 - L-gamma-glutamyl-p-nitroanilide wild-type, 37°C 673687 2.6.1.16 0.072 - L-glutamine mutant enzyme V605L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.072 - L-glutamine mutant V605L, 37°C 673687 2.6.1.16 0.1 - L-gamma-glutamyl-p-nitroanilide mutant W74L, 37°C 673687 2.6.1.16 0.1 - L-glutamine mutant A602L, 37°C 673687 2.6.1.16 0.1 - L-glutamine mutant enzyme A602L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.11 - L-glutamine synthase activity, pH 7.2, 37°C 737433 2.6.1.16 0.14 - L-glutamine hemisynthase activity, pH 7.2, 37°C 737433 2.6.1.16 0.17 - L-glutamine wild type enzyme 672444 2.6.1.16 0.2 - D-fructose 6-phosphate wild type enzyme 672444 2.6.1.16 0.2 - L-gamma-glutamyl-p-nitroanilide mutant W74A, 37°C 673687 2.6.1.16 0.2 - L-glutamate calorimetric determination, pH 7.2, 25°C 721419 2.6.1.16 0.2 - L-glutamine pH 7.4, 37°C 639920 2.6.1.16 0.2 - L-glutamine pH 7.5, 37°C 684404 2.6.1.16 0.225 - L-glutamine pH 7.5, 37°C 639911 2.6.1.16 0.23 - D-fructose 6-phosphate calorimetric determination, pH 7.2, 37°C 721419 2.6.1.16 0.25 - D-fructose 6-phosphate pH 7.5, 37°C 684404 2.6.1.16 0.27 - L-glutamine wild type enzyme, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.27 - L-glutamine wild-type, 37°C 673687 2.6.1.16 0.29 - L-gamma-glutamyl-p-nitroanilide mutant V605L, 37°C 673687 2.6.1.16 0.3 - D-fructose 6-phosphate calorimetric determination, pH 7.2, 25°C 721419 2.6.1.16 0.3 - L-glutamine pH 6.3, 38°C 639918 2.6.1.16 0.3 - L-glutamine pH 7.5, 37°C 639910 2.6.1.16 0.31 - L-gamma-glutamyl-p-nitroanilide residues 1-345, His-tagged glutamine amide-hydrolysing domain, pH 7.5, 25°C 671896 2.6.1.16 0.34 - L-glutamate reaction mixture contains 10 mM L-glutamate, 1 mM EDTA, 1 mM DTT and an appropriately diluted enzyme preparation in 20 mM HEPES buffer (pH 7.5) at 25°C 671896 2.6.1.16 0.34 - L-glutamine glutamine 6-phosphate-synthetic activity, wild-type, pH 7.5, 25°C 671896 2.6.1.16 0.36 - D-fructose 6-phosphate wild type enzyme, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.36 - D-fructose 6-phosphate wild-type, 37°C 673687 2.6.1.16 0.36 - L-gamma-glutamyl-p-nitroanilide mutant A602L, 37°C 673687 2.6.1.16 0.38 - D-fructose 6-phosphate pH 7.7, 37°C 639904 2.6.1.16 0.38 - L-glutamine mutant enzyme W74A, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.38 - L-glutamine mutant W74A, 37°C 673687 2.6.1.16 0.39 - D-fructose 6-phosphate pH 7.0, 37°C 722052 2.6.1.16 0.4 - D-fructose 6-phosphate - 639931 2.6.1.16 0.4 - L-glutamine pH 7.5, 37°C 639911, 639922 2.6.1.16 0.5 - D-fructose 6-phosphate pH 6.3, 38°C 639918 2.6.1.16 0.5 - D-fructose 6-phosphate synthase activity, pH 7.2, 37°C 737433 2.6.1.16 0.5 - L-glutamine pH 6.5, 30°C 639923 2.6.1.16 0.52 - D-fructose 6-phosphate mutant S243E, 37°C, pH 7.5 685109 2.6.1.16 0.55 - D-fructose 6-phosphate recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol 706608 2.6.1.16 0.6 - L-glutamine pH 7.5, 37°C 639911 2.6.1.16 0.64 - L-gamma-glutamyl-p-nitroanilide wild-type with C-terminal His-tag, pH 7.5, 25°C 671896 2.6.1.16 0.65 - L-glutamine pH 7.0, 37°C 639904 2.6.1.16 0.67 - D-fructose 6-phosphate mutant enzyme V605L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.67 - D-fructose 6-phosphate mutant V605L, 37°C 673687 2.6.1.16 0.67 - L-gamma -glutamyl-p-nitroanilide reaction mixture contains 1 mM L-gamma-glutamyl-p-nitroanilide, 1 mM EDTA, 1 mM DTT and the appropriately diluted enzyme preparation in 20 mM HEPES buffer (pH 7.5) at 25°C 671896 2.6.1.16 0.67 - L-gamma-glutamyl-p-nitroanilide wild-type, pH 7.5, 25°C 671896 2.6.1.16 0.74 - L-glutamine pH 7.0, 37°C 722052 2.6.1.16 0.75 - D-fructose 6-phosphate pH 7.0, 37°C 639904 2.6.1.16 0.75 - L-glutamine recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol 706608 2.6.1.16 0.8 - D-fructose 6-phosphate hemisynthase activity, pH 7.2, 37°C 737433 2.6.1.16 0.8 - L-glutamine - 639931 2.6.1.16 0.85 - L-glutamine pH 7.5, 37°C 639911 2.6.1.16 0.93 - D-fructose 6-phosphate mutant enzyme W74A, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 0.93 - D-fructose 6-phosphate mutant W74A, 37°C 673687 2.6.1.16 1 - L-glutamine - 661954 2.6.1.16 1.04 - D-fructose 6-phosphate wild-type, 37°C, pH 7.5 685109 2.6.1.16 1.08 - D-fructose 6-phosphate hexose phosphate-isomerizing activity, wild-type with N-terminal His-tag, pH 7.5, 25°C 671896 2.6.1.16 1.11 - D-fructose 6-phosphate mutant enzyme W74L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 1.11 - D-fructose 6-phosphate mutant W74L, 37°C 673687 2.6.1.16 1.12 - D-fructose 6-phosphate mutant mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C 723015 2.6.1.16 1.15 - D-fructose 6-phosphate mutant A602L, 37°C 673687 2.6.1.16 1.15 - D-fructose 6-phosphate mutant enzyme A602L, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 1.2 - D-fructose 6-phosphate hexose phosphate-isomerizing activity, wild-type, pH 7.5, 25°C 671896 2.6.1.16 1.2 - D-fructose 6-phosphate reaction mixture contains 2 mM D-fructose 6-phosphate, 0.5 mM NADP+, 1 mM EDTA, and appropriately diluted GlcN-6-P synthase preparation in 50 mM Tris/HCl buffer (pH 7.5) at 25°C 671896 2.6.1.16 1.2 - D-fructose 6-phosphate wild type enzyme, in in 25 mM potassium phosphate buffer at pH 7.0 and 37°C 676955 2.6.1.16 1.33 - L-glutamate mutant Gfa1-K568H/S569H, pH 6.8, 37°C 723015 2.6.1.16 1.36 - L-glutamate mutant Gfa1-His6DELTA343-348, pH 6.8, 37°C 723015 2.6.1.16 1.4 - D-fructose 6-phosphate hexose phosphate-isomerizing activity, residues 346-712, His-tagged hexose phosphate-isomerizing domain, pH 7.5, 25°C 671896 2.6.1.16 1.41 - D-fructose 6-phosphate glutamine 6-phosphate-synthetic activity, wild-type, pH 7.5, 25°C 671896 2.6.1.16 1.41 - L-glutamate wild-type, pH 6.8, 37°C 723015 2.6.1.16 1.45 - D-fructose 6-phosphate mutant enzyme W74F, in 50 mM potassium phosphate, pH 7.5, 1 mM EDTA, 50 mM KCl, at 37°C 673687 2.6.1.16 1.45 - D-fructose 6-phosphate mutant W74F, 37°C 673687 2.6.1.16 1.56 - D-fructose 6-phosphate wild-type, pH 6.8, 37°C 723015 2.6.1.16 1.6 - L-glutamine pH 7.7, 37°C 639904 2.6.1.16 1.8 - L-gamma-glutamyl-p-nitroanilide both wild-type and mutant S243E, 37°C, pH 7.5 685109 2.6.1.16 1.9 - D-fructose 6-phosphate mutant mutant Gfa1-K568H/S569H, pH 6.8, 37°C 723015 2.6.1.16 2 - D-fructose 6-phosphate pH 7.0, 37°C 639904 2.6.1.16 2 - D-fructose 6-phosphate pH 7.5, 37°C 639922 2.6.1.16 2.36 - L-glutamate mutant Gfa1-His6DELTA655-660, pH 6.8, 37°C 723015 2.6.1.16 2.6 - D-fructose 6-phosphate mutant Gfa1-His6DELTA343-348, pH 6.8, 37°C 723015 2.6.1.16 2.8 - D-fructose 6-phosphate pH 7.0, 37°C 737920 2.6.1.16 3.8 - D-fructose 6-phosphate pH 6.5, 30°C 639923 2.6.1.16 3.8 - D-fructose 6-phosphate pH 7.2, 37°C, isomerization catalyzed by C-terminal domain 639933 2.6.1.16 5.1 - L-glutamine pH 7.0, 55°C 684758 2.6.1.16 6.5 - D-fructose 6-phosphate hexose phosphate-isomerizing activity, wild-type with C-terminal His-tag, pH 7.5, 25°C 671896 2.6.1.16 12.2 - L-gamma-glutamyl-p-nitroanilide wild-type with N-terminal His-tag, pH 7.5, 25°C 671896 2.6.1.16 16 - D-glucose 6-phosphate pH 7.2, 37°C, isomerization catalyzed by C-terminal domain 639933