2.5.1.6 additional information - additional information different isoforms of MAT differ in kinetic and regulatory properties, overview 675169 2.5.1.6 additional information - additional information high performance liquid chromatography assay method using catechol-O-methyltransferase-coupled fluorometric detection, negative cooperativity of enzyme with Hill coefficinet of 0.5 657534 2.5.1.6 0.0013 - tripolyphosphate wild type, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine 639076 2.5.1.6 0.0015 - tripolyphosphate G7 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine 639076 2.5.1.6 0.0016 - tripolyphosphate G8 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine 639076 2.5.1.6 0.002 - ATP beta form from liver 639058 2.5.1.6 0.0023 - L-methionine pH 8.0, 37°C 727487 2.5.1.6 0.0028 - L-methionine pH 8.0, 65°C 727487 2.5.1.6 0.0029 - L-methionine - 639069 2.5.1.6 0.003 - L-methionine - 639069 2.5.1.6 0.003 - tripolyphosphate wild type, tripolyphosphatase activity, in absence of S-adenosylmethionine 639076 2.5.1.6 0.0032 - tripolyphosphate G6 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine 639076 2.5.1.6 0.005 - ATP alpha form from liver 639058 2.5.1.6 0.0053 - tripolyphosphate G5 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine 639076 2.5.1.6 0.0056 - L-ethionine pH 8.0, 37°C 727487 2.5.1.6 0.006 - Mg2+ alpha form from liver 639058 2.5.1.6 0.0061 - L-methionine pH 7.0, 37°C 657534 2.5.1.6 0.007 - L-ethionine pH 8.0, 65°C 727487 2.5.1.6 0.007 - Mg2+ beta form from liver 639058 2.5.1.6 0.0075 - L-methionine erythrocyte extract, alpha and beta subunit 639059 2.5.1.6 0.008 - tripolyphosphate G8 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine 639076 2.5.1.6 0.0083 - methionine MAT-II isoenzyme 639043 2.5.1.6 0.01 - ATP pH 8, 37°C, mutant enzyme I303V/I65V/L186V 759131 2.5.1.6 0.01 - ATP pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K 759131 2.5.1.6 0.01 - L-methionine - 639057 2.5.1.6 0.01 - L-methionine isoenzyme A 639048 2.5.1.6 0.01 - L-methionine S-adenosylmethionine synthetase A 639048 2.5.1.6 0.011 - tripolyphosphate G6 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine 639076 2.5.1.6 0.0125 - L-methionine erythrocyte extract, alpha subunit 639059 2.5.1.6 0.014 - tripolyphosphate RLL mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine 639076 2.5.1.6 0.0145 - ATP pH 8.0, 65°C, cosubstrate: L-methionine 727487 2.5.1.6 0.015 - L-methionine - 639065 2.5.1.6 0.015 - L-methionine endogenous MAT II 639059 2.5.1.6 0.015 - tripolyphosphate G7 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine 639076 2.5.1.6 0.016 - L-methionine alpha2-transfected MAT II, two kinetic forms 639065 2.5.1.6 0.017 - L-methionine alpha form from liver 639058 2.5.1.6 0.02 - L-methionine isoenzyme B 639048 2.5.1.6 0.02 - L-methionine recombinant MAT II co-expressing alpha2 and beta subunits 639065 2.5.1.6 0.022 - L-methionine crude extract 639059 2.5.1.6 0.022 - L-methionine one kinetic form of alpha-two subunit in the presence of beta subunit 639070 2.5.1.6 0.022 0.024 L-methionine ro subunit 639059 2.5.1.6 0.024 - L-methionine S-adenosylmethionine synthetase B 639048 2.5.1.6 0.024 - L-methionine wild-type 639067 2.5.1.6 0.024 - tripolyphosphate G5 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine 639076 2.5.1.6 0.026 - ATP G6 mutant, S-adenosylmethionine synthesis 639076 2.5.1.6 0.026 - tripolyphosphate RLL mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine 639076 2.5.1.6 0.03 - L-methionine mutant D249N, pH 8.0 658640 2.5.1.6 0.03 0.038 L-methionine one kinetic form of alpha subunit in the presence of beta subunit 639070 2.5.1.6 0.031 - ATP - 639052 2.5.1.6 0.031 - L-methionine pH 8.0, 80°C 728983 2.5.1.6 0.034 - L-methionine pH 8.4, 37°C 759436 2.5.1.6 0.036 - L-methionine mutant D166N, pH 8.0 658640 2.5.1.6 0.038 - L-methionine mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.04 - L-methionine wild-type, pH 8.0 658640 2.5.1.6 0.041 - L-methionine MAT-I isoenzyme 639043 2.5.1.6 0.041 - L-methionine mutant K280A, pH 8.0 658640 2.5.1.6 0.043 - L-methionine pH 8.4, 37°C, mutant enzyme 759436 2.5.1.6 0.045 - ATP RLL and G8 mutants, S-adenosylmethionine synthesis 639076 2.5.1.6 0.045 - L-methionine mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.046 - L-methionine pH 8.4, 37°C, mutant enzyme 759436 2.5.1.6 0.047 - L-methionine pH 8.0, 37°C, mutant enzyme Q113D 758565 2.5.1.6 0.05 - ATP - 639057 2.5.1.6 0.05 - ATP isoenzyme A 639048 2.5.1.6 0.05 - ATP pH 8, 37°C, mutant enzyme I303V/I65V 759131 2.5.1.6 0.05 - L-methionine mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.051 - L-methionine pH 8.4, 37°C, mutant enzyme 759436 2.5.1.6 0.0592 - ATP pH 8.0, 65°C, cosubstrate: L-ethionine 727487 2.5.1.6 0.06 - L-methionine uninduced E. coli NM522 strain extract alpha subunit at low L-methionine concentrations 639060 2.5.1.6 0.06 0.1 L-methionine alpha-two subunit 639070 2.5.1.6 0.065 - ATP S-adenosylmethionine synthetase A 639048 2.5.1.6 0.065 0.08 L-methionine alpha subunit at low L-methionine concentrations 639070 2.5.1.6 0.0686 - ATP pH 8.0, 37°C, cosubstrate: L-methionine 727487 2.5.1.6 0.069 - ATP G7 mutants, S-adenosylmethionine synthesis 639076 2.5.1.6 0.073 - ATP wild-type S-adenosylmethionine synthesis 639076 2.5.1.6 0.074 - L-methionine mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.075 - L-methionine alpha2-transfected MAT II, two kinetic forms 639065 2.5.1.6 0.076 - ATP 37°C, pH 8.2 658351 2.5.1.6 0.076 - L-methionine one kinetic form of alpha-two subunit in the presence of beta subunit 639070 2.5.1.6 0.078 - L-methionine pH 8.0, 37°C, mutant enzyme K289L 758565 2.5.1.6 0.08 - ATP - 639039 2.5.1.6 0.08 - L-methionine induced E. coli NM522 strain extract alpha subunit at low L-methionine concentrations and uninduced E. coli NM522 strain extract alpha subunit at high L-methionine concentrations 639060 2.5.1.6 0.08 - L-methionine pH 8, 37°C, wild-type enzyme 759131 2.5.1.6 0.08 - L-methionine S283T mutant 639067 2.5.1.6 0.08 0.09 L-methionine alpha subunit at high L-methionine concentrations. Also one kinetic form of alpha subunit in the presence of beta subunit 639070 2.5.1.6 0.083 - ATP wild-type, pH 8.0 657653 2.5.1.6 0.087 - ATP G5 mutant S-adenosylmethionine synthesis 639076 2.5.1.6 0.088 - L-methionine Q113A mutant, S-adenosylmethionine synthesis 639067 2.5.1.6 0.092 - L-methionine wild-type, S-adenosylmethionine synthesis 639076 2.5.1.6 0.096 - L-methionine pH 8.2, 37°C 658771 2.5.1.6 0.0969 - ATP pH 8.0, 37°C, cosubstrate: L-ethionine 727487 2.5.1.6 0.1 - ATP mutant W387F/Y170W, 55°C 702442 2.5.1.6 0.1 - ATP mutant W387F/Y226W, 55°C 702442 2.5.1.6 0.1 - ATP pH 8, 37°C, mutant enzyme I303V 759131 2.5.1.6 0.1 - L-methionine - 639063 2.5.1.6 0.11 - ATP mutant W387F, 55°C 702442 2.5.1.6 0.11 - ATP mutant W387F/Y371W, 55°C 702442 2.5.1.6 0.11 - ATP mutant W387F/Y72W, 55°C 702442 2.5.1.6 0.11 - L-methionine pH 8.0, 37°C 673389 2.5.1.6 0.11 - L-methionine wild-type, pH 8.0 657653 2.5.1.6 0.115 - ATP pH 8.0, 80°C 728983 2.5.1.6 0.12 - L-methionine 37°C, pH 8.0, mutant enzyme I303V 759009 2.5.1.6 0.12 - L-methionine pH 8.0, 37°C 706910 2.5.1.6 0.13 - ATP mutant D107C, pH 8.0 657653 2.5.1.6 0.13 - ATP mutant G105C, pH 8.0 657653 2.5.1.6 0.13 - ATP mutant W387F/Y120W, 55°C 702442 2.5.1.6 0.13 - L-methionine mutant W387F/Y323W, 55°C 702442 2.5.1.6 0.13 - L-methionine mutant W387F/Y72W, 55°C 702442 2.5.1.6 0.137 - L-methionine mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.137 - L-methionine mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.14 - L-methionine pH 8.0, 37°C, wild-type enzyme 758565 2.5.1.6 0.14 - L-methionine pH 8.0, 58°C 727832 2.5.1.6 0.141 - L-methionine mutant D282N, pH 8.0 658640 2.5.1.6 0.147 - L-methionine mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.15 - ATP isoenzyme B 639048 2.5.1.6 0.156 - ATP S-adenosylmethionine synthetase B 639048 2.5.1.6 0.17 - ATP pH 8, 37°C, wild-type enzyme 759131 2.5.1.6 0.17 - L-methionine mutant K256A, pH 8.0 658640 2.5.1.6 0.17 - L-methionine mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.17 - L-methionine pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K 759131 2.5.1.6 0.176 - ATP pH 8.4, 37°C, mutant enzyme 759436 2.5.1.6 0.176 - L-methionine mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.18 - ATP - 639063 2.5.1.6 0.18 - ATP mutant G105R1, pH 8.0 657653 2.5.1.6 0.18 - ATP mutant W387F/Y49W, 55°C 702442 2.5.1.6 0.18 - L-methionine 37°C, pH 8.0, wild-type enzyme 759009 2.5.1.6 0.18 - L-methionine mutant D107R1, pH 8.0 657653 2.5.1.6 0.19 - ATP mutant W387F/Y255W, 55°C 702442 2.5.1.6 0.19 - ATP wild-type, 55°C 702442 2.5.1.6 0.19 - L-methionine mutant D107R1, pH 8.0 657653 2.5.1.6 0.2 - ATP mutant W387F/Y233W, 55°C 702442 2.5.1.6 0.2 - ATP mutant W387F/Y267W, 55°C 702442 2.5.1.6 0.2 - L-methionine induced E. coli NM522 strain extract alpha subunit at high L-methionine concentrations 639060 2.5.1.6 0.206 - ATP pH 8.4, 37°C, mutant enzyme 759436 2.5.1.6 0.21 - ATP mutant W387F/Y85W, 55°C 702442 2.5.1.6 0.215 - L-methionine MAT-III isoenzyme 639043 2.5.1.6 0.22 - ATP mutant W387F/Y323W, 55°C 702442 2.5.1.6 0.22 - ATP mutant W387F/Y344W, 55°C 702442 2.5.1.6 0.22 - ATP saturated with KCl 639074 2.5.1.6 0.22 - L-methionine wild-type 639067 2.5.1.6 0.222 - methionine mutant C35S, presence of dithiothreitol 659298 2.5.1.6 0.23 - L-methionine RLL mutant, S-adenosylmethionine synthesis 639076 2.5.1.6 0.24 - ATP mutant W387F/Y273W, 55°C 702442 2.5.1.6 0.24 - L-methionine - 639074 2.5.1.6 0.246 - methionine wild-type, presence of dithiothreitol 659298 2.5.1.6 0.25 - ATP - 639063 2.5.1.6 0.25 - ATP pH 8.0, 58°C 727832 2.5.1.6 0.25 - L-methionine mutant W387F/Y371W, 55°C 702442 2.5.1.6 0.26 - ATP - 639074 2.5.1.6 0.26 - L-methionine in 100 mM Tris-Cl pH 8.0, 20 mM MgCl2, at 37°C 685392 2.5.1.6 0.287 - L-methionine wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 0.288 - L-methionine 37°C, pH 8.2 658351 2.5.1.6 0.29 - L-methionine mutant D107C, pH 8.0 657653 2.5.1.6 0.3 - L-methionine G6 mutant, S-adenosylmethionine synthesis 639076 2.5.1.6 0.3 - L-methionine mutant W387F/Y226W, 55°C 702442 2.5.1.6 0.3 - L-methionine mutant W387F/Y273W, 55°C 702442 2.5.1.6 0.3 - L-methionine pH 8, 37°C, mutant enzyme I303V/I65V/L186V 759131 2.5.1.6 0.3 - L-methionine saturated with KCl 639074 2.5.1.6 0.3 - MgATP2- ATP in form of MgATP2- 639040 2.5.1.6 0.31 - ATP - 639063 2.5.1.6 0.31 - L-methionine at pH 8.0 and 37°C 737953 2.5.1.6 0.31 - L-methionine mutant W387F, 55°C 702442 2.5.1.6 0.31 - L-methionine wild-type, 55°C 702442 2.5.1.6 0.33 - ATP pH 8.0, 37°C 706910 2.5.1.6 0.33 - L-methionine mutant W387F/Y344W, 55°C 702442 2.5.1.6 0.34 - ATP 37°C, pH 8.0, mutant enzyme I303V 759009 2.5.1.6 0.34 - ATP pH 8.0, 37°C 673389 2.5.1.6 0.39 - S-(-)-methioninol pH 8.0, 37°C, mutant enzyme Q113D 758565 2.5.1.6 0.4 - CTP - 639074 2.5.1.6 0.4 - methionine - 639040 2.5.1.6 0.42 - L-methionine pH 8, 37°C, mutant enzyme I303V 759131 2.5.1.6 0.43 - ATP 37°C, pH 8.0, wild-type enzyme 759009 2.5.1.6 0.43 - L-methionine pH 8, 37°C, mutant enzyme I303V/I65V 759131 2.5.1.6 0.449 - methionine mutant C35S, presence of glutathione 659298 2.5.1.6 0.45 - L-methionine mutant G105C, pH 8.0 657653 2.5.1.6 0.45 - L-methionine mutant W387F/Y267W, 55°C 702442 2.5.1.6 0.47 - L-methionine mutant W387F/Y233W, 55°C 702442 2.5.1.6 0.49 - L-methionine G7 mutant, S-adenosylmethionine synthesis 639076 2.5.1.6 0.493 - ATP mutant C35S, presence of dithiothreitol 659298 2.5.1.6 0.5 - L-methionine mutant W387F/Y255W, 55°C 702442 2.5.1.6 0.5 - methionine beta form from liver 639058 2.5.1.6 0.51 - L-methionine at pH 8.5 and 35°C 737893 2.5.1.6 0.527 - L-methionine at pH 8.0 and 35°C 685773 2.5.1.6 0.53 - ATP wild-type, presence of glutathione 659298 2.5.1.6 0.54 - L-methionine mutant W387F/Y120W, 55°C 702442 2.5.1.6 0.56 - ATP pH 8.4, 37°C 759436 2.5.1.6 0.57 - L-methionine mutant W387F/Y85W, 55°C 702442 2.5.1.6 0.588 - ATP wild-type, presence of dithiothreitol 659298 2.5.1.6 0.62 - GTP - 639074 2.5.1.6 0.62 - L-methionine G8 mutant, S-adenosylmethionine synthesis 639076 2.5.1.6 0.631 - ATP at pH 8.0 and 35°C 685773 2.5.1.6 0.66 - L-methionine mutant W387F/Y49W, 55°C 702442 2.5.1.6 0.68 - ATP pH 8.4, 37°C, mutant enzyme 759436 2.5.1.6 0.74 - L-ethionine - 639074 2.5.1.6 0.75 - L-methionine mutant G105R1, pH 8.0 657653 2.5.1.6 0.756 - methionine mutant C61S, presence of dithiothreitol 659298 2.5.1.6 0.77 - L-methionine G5 mutant, S-adenosylmethionine synthesis 639076 2.5.1.6 0.778 - ATP mutant C35S, presence of glutathione 659298 2.5.1.6 0.794 - methionine mutant C61S, presence of glutathione 659298 2.5.1.6 0.85 - L-methionine at pH 8.5 and 45°C 737471 2.5.1.6 0.92 - ATP in 100 mM Tris-Cl pH 8.0, 20 mM MgCl2, at 37°C 685392 2.5.1.6 1 - L-methionine mutant W387F/Y170W, 55°C 702442 2.5.1.6 1 - S-(-)-methioninol pH 8.0, 37°C, mutant enzyme K289L 758565 2.5.1.6 1.1 - L-methionine - 639074 2.5.1.6 1.12 - methionine wild-type, presence of glutathione 659298 2.5.1.6 1.2 - L-methionine at pH 8.0 and 70°C 738285 2.5.1.6 1.4 - ATP - 639074 2.5.1.6 1.4 - ITP pH 8.0, 58°C 727832 2.5.1.6 1.47 - L-methionine at pH 8.5 and 40°C 738280 2.5.1.6 1.72 - ATP at pH 8.5 and 45°C 737471 2.5.1.6 2.07 - ATP at pH 8.5 and 35°C 737893 2.5.1.6 2.2 - UTP - 639074 2.5.1.6 2.4 - S-(-)-methioninol pH 8.0, 37°C, wild-type enzyme 758565 2.5.1.6 2.54 - ATP mutant C61S, presence of glutathione 659298 2.5.1.6 2.6 - L-methionine methyl esther - 639074 2.5.1.6 2.84 - ATP at pH 8.5 and 40°C 738280 2.5.1.6 3.25 - ATP mutant C61S, presence of dithiothreitol 659298 2.5.1.6 3.3 - L-methionine - 639052 2.5.1.6 3.5 - D-methionine - 639074 2.5.1.6 4.19 - ATP at pH 8.0 and 70°C 738285 2.5.1.6 6.54 - ATP at pH 8.0 and 37°C 737953