2.4.2.29 additional information - additional information kinetic parameters of recombinant and native enzyme 728740 2.4.2.29 0.000014 - 7-(aminomethyl)-7-deazaguanine - 644917 2.4.2.29 0.000053 - guanine - 644917 2.4.2.29 0.0001 - guanine histidine-tagged wild-type enzyme, pH 7.3 644933 2.4.2.29 0.00011 - tRNA histidine-tagged mutant enzyme D89E, pH 7.3 644933 2.4.2.29 0.00012 - tRNA histidine-tagged wild-type enzyme, pH 7.3 644933 2.4.2.29 0.00016 - guanine34 in tRNA at pH 7.3 689250 2.4.2.29 0.00032 - tRNA(Tyr) wild-type enzyme 644934 2.4.2.29 0.00036 - [tRNA]-guanine mutant Y106F 703226 2.4.2.29 0.00038 - guanine wild-type 703226 2.4.2.29 0.00039 - tRNA histidine-tagged wild-type enzyme, pH 8.5 644933 2.4.2.29 0.0005 - pre-queuine 0 mutant enzyme E235Q, Km less than 0.0005 mM, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00051 - tRNA histidine-tagged mutant enzyme D89E, pH 8.5 644933 2.4.2.29 0.00057 - guanine histidine-tagged wild-type enzyme, pH 8.5 644933 2.4.2.29 0.0007 - pre-queuine 1 wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00087 - virF minihelix RNA at pH 7.3 689250 2.4.2.29 0.0009 - pre-queuine 0 wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.0009 - tRNA guanine wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00098 - [tRNATyr]7-aminomethyl-7-carbaguanine mutant K52M 705164 2.4.2.29 0.001 - tRNA guanine mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.001 - [tRNA]-guanine wild-type 703226 2.4.2.29 0.0011 - guanine mutant enzyme S90A 644934 2.4.2.29 0.0012 - guanine wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.0013 - guanine mutant Y106F 703226 2.4.2.29 0.00158 - [tRNATyr]7-aminomethyl-7-carbaguanine mutant Y330F 705164 2.4.2.29 0.0018 - virF mRNA at pH 7.3 689250 2.4.2.29 0.00217 - [tRNATyr]7-aminomethyl-7-carbaguanine wild-type 705164 2.4.2.29 0.00261 - guanine histidine-tagged mutant enzyme D89E, pH 7.3 644933 2.4.2.29 0.00262 - [RNAECYMA]-2'-deoxyguanine 34 - 702263 2.4.2.29 0.00287 - [RNAECYMA]-guanine - 702263 2.4.2.29 0.003 - guanine wild-type enzyme 644934 2.4.2.29 0.0033 - guanine mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00632 - guanine histidine-tagged mutant enzyme D89E, pH 8.5 644933 2.4.2.29 0.0097 - tRNA(Tyr) mutant enzyme S90C 644934 2.4.2.29 0.032 - pre-queuine 1 mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.152 - 2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one - 644935