2.2.1.6	additional information	-	additional information	activity is dependent on the ionic strength of the buffer and diminishes considerably (approximately 80%) when assayed in buffers with less than 100 mM concentrations. At concentrations higher than 100 mM the activity levels are quite similar (tested up to 500 mM)	733241	
2.2.1.6	additional information	-	additional information	binding kinetics of Mg2+ and thiamine diphosphate with wild-type enzyme and mutant enzymes, overview	733987	
2.2.1.6	additional information	-	additional information	cofactor affinities of wild-type and mutant enzymes, overview	672866	
2.2.1.6	additional information	-	additional information	kinetics	671290, 671854, 673626	
2.2.1.6	additional information	-	additional information	kinetics of isozymes	673213	
2.2.1.6	additional information	-	additional information	kinetics of wild-type and mutant enzymes	674354	
2.2.1.6	additional information	-	additional information	kinetics or recombinant wild-type and reconstituted isozymes AHAS I, exclusive binding model	672368	
2.2.1.6	additional information	-	additional information	Michaelis-Menten kinetics and optimal reaction conditions, overview	733744	
2.2.1.6	additional information	-	additional information	Michaelis-Menten steady-state kinetic analysis, overview	733241	
2.2.1.6	additional information	-	additional information	non-hyperbolic substrate-saturation curve, involving interaction between the active sites of the dimer	395902	
2.2.1.6	additional information	-	additional information	steady-state kinetics at different reaction conditions	672865	
2.2.1.6	additional information	-	additional information	steady-state kinetics of recombinant wild-type and mutant enzymes, cofactor binding parameters, overview	672868	
2.2.1.6	additional information	-	additional information	substrate and cofactor kinetics of wild-type and mutant enzymes, overview	733597	
2.2.1.6	0.0028	-	pyruvate	pH 7.5, 37°C, recombinant His6-tagged mutant H84A	733747	
2.2.1.6	0.0028	-	pyruvate	pH 7.5, 37°C, recombinant His6-tagged mutant H84T	733747	
2.2.1.6	0.0028	-	pyruvate	pH 7.5, 37°C, recombinant His6-tagged mutant Q86A	733747	
2.2.1.6	0.0028	-	pyruvate	pH 7.5, 37°C, recombinant His6-tagged wild-type enzyme	733747	
2.2.1.6	0.0028	-	pyruvate	wild type enzyme, at pH 7.5 and 37°C	733747	
2.2.1.6	0.0117	-	pyruvate	wild-type enzyme	672866	
2.2.1.6	0.0289	-	pyruvate	mutant R372S/F373P/D374V/D375E	672866	
2.2.1.6	0.0757	-	pyruvate	mutant R372S/F373P/D374V/D375E/R376Y	672866	
2.2.1.6	0.141	-	pyruvate	mutant enzyme Q86A, at pH 7.5 and 37°C	733747	
2.2.1.6	0.15	-	pyruvate	mutant enzyme H84A, at pH 7.5 and 37°C	733747	
2.2.1.6	0.213	-	pyruvate	mutant enzyme H84T, at pH 7.5 and 37°C	733747	
2.2.1.6	0.28	-	thiamine diphosphate	-	697061	
2.2.1.6	0.33	-	pyruvate	mutant C83T, pH 7.6, 37°C	719466	
2.2.1.6	0.5	-	pyruvate	-	395875	
2.2.1.6	1	-	2-oxobutanoate	at 1.5 mM pyruvate	395917	
2.2.1.6	1	-	pyruvate	pH 7.6, isoenzyme AHAS I	395876	
2.2.1.6	1.08	-	pyruvate	mutant F147R, pH 7.5, 37°C	719375	
2.2.1.6	1.09	-	pyruvate	mutant enzyme W574L, at pH 7.5 and 37°C	757919	
2.2.1.6	1.1	-	pyruvate	mutant Q487S, pH 7.0, 37°C	695784	
2.2.1.6	1.18	-	pyruvate	wild-type, pH 7.5, 37°C	719375	
2.2.1.6	1.19	-	pyruvate	mutant enzyme P197T, at pH 7.5 and 37°C	757919	
2.2.1.6	1.2	-	pyruvate	mutant C83S, pH 7.6, 37°C	719466	
2.2.1.6	1.3	-	pyruvate	isoenzyme I	395887	
2.2.1.6	1.37	-	2-oxobutanoate	pH 7.5, 37°C	658364	
2.2.1.6	1.37	-	pyruvate	at pH 6.8 and 37°C	733744	
2.2.1.6	1.37	-	pyruvate	mutant enzyme P197L, at pH 7.5 and 37°C	757919	
2.2.1.6	1.37	-	pyruvate	pH 6.8, 37°C, recombinant enzyme	733744	
2.2.1.6	1.37	-	pyruvate	pH 7.5, 37°C, recombinant wild-type enzyme	733987	
2.2.1.6	1.37	-	pyruvate	recombinant wild-type enzyme, pH and temperature not specified in the publication	733597	
2.2.1.6	1.37	-	pyruvate	wild type enzyme, at pH 6.8 and 37°C	733987	
2.2.1.6	1.37	-	pyruvate	wild type enzyme, pH and temperature not specified in the publication	733597	
2.2.1.6	1.38	-	pyruvate	mutant enzyme P197H, at pH 7.5 and 37°C	757919	
2.2.1.6	1.38	-	pyruvate	mutant S539R, pH not specified in the publication, temperature not specified in the publication	719010	
2.2.1.6	1.46	-	pyruvate	mutant enzyme P197S, at pH 7.5 and 37°C	757919	
2.2.1.6	1.5	-	pyruvate	mutant C83A, pH 7.6, 37°C	719466	
2.2.1.6	1.5	2	pyruvate	mutant enzyme D376E, at pH 7.5 and 37°C	757919	
2.2.1.6	1.56	-	pyruvate	pH 7.0, 37°C, holoenzyme	673626	
2.2.1.6	1.6	-	pyruvate	mutant Q487G, pH 7.0, 37°C	695784	
2.2.1.6	1.7	-	pyruvate	mutant enzyme E49D, pH and temperature not specified in the publication	733597	
2.2.1.6	1.7	-	pyruvate	recombinant mutant E49D, pH and temperature not specified in the publication	733597	
2.2.1.6	1.74	-	pyruvate	wild type enzyme, at pH 7.5 and 37°C	757919	
2.2.1.6	1.89	-	pyruvate	mutant L141A, pH 7.5, 37°C	719375	
2.2.1.6	2.1	-	pyruvate	-	395883	
2.2.1.6	2.2	-	pyruvate	pH 7.0, 30°C, sulfonylurea-resistant biotype	660058	
2.2.1.6	2.3	-	pyruvate	-	395875	
2.2.1.6	2.49	-	pyruvate	wild-type, pH 7.5, 37°C	719375	
2.2.1.6	2.6	-	pyruvate	native protein	395904	
2.2.1.6	2.76	-	pyruvate	catalytic subunit	672867	
2.2.1.6	2.76	-	pyruvate	pH 7.0, 37°C, catalytic subunit	673626	
2.2.1.6	2.76	-	pyruvate	pH 7.5, 37°C, recombinant wild-type enzyme	733745	
2.2.1.6	2.76	-	pyruvate	wild type enzyme, at pH 7.5 and 37°C	733745	
2.2.1.6	2.9	-	pyruvate	fusion protein containing an N-terminal oligohistidine sequence on the large subunit	395904	
2.2.1.6	3	-	pyruvate	-	395916	
2.2.1.6	3.2	-	pyruvate	-	395896	
2.2.1.6	3.33	-	pyruvate	mutant S539A, pH not specified in the publication, temperature not specified in the publication	719010	
2.2.1.6	3.35	-	pyruvate	mutant L89A, pH 7.5, 37°C	719375	
2.2.1.6	3.36	-	pyruvate	mutant S167A, pH not specified in the publication, temperature not specified in the publication	719010	
2.2.1.6	3.6	-	pyruvate	mutant D375E, pH 7.5, 37°C	658223	
2.2.1.6	3.6	-	pyruvate	mutant L476M/Q480W, pH 7.6, 37°C	719466	
2.2.1.6	3.66	-	pyruvate	pH 7.5, 37°C	658364	
2.2.1.6	3.7	-	pyruvate	pH 7.0, 30°C, sulfonylurea-susceptible biotype	660058	
2.2.1.6	3.89	-	pyruvate	mutant F147A, pH 7.5, 37°C	719375	
2.2.1.6	3.93	-	pyruvate	-	395915	
2.2.1.6	3.96	-	pyruvate	at pH 6.5 and 37°C	756209	
2.2.1.6	4	-	pyruvate	37°C, reconstituted, recombinant holoenzyme	672368	
2.2.1.6	4.15	-	pyruvate	pH 7.6, 37°C	719117	
2.2.1.6	4.2	-	pyruvate	-	395875	
2.2.1.6	4.34	-	pyruvate	mutant enzyme Q411N, at pH 6.8 and 37°C	733987	
2.2.1.6	4.34	-	pyruvate	pH 7.5, 37°C, recombinant mutant Q411N	733987	
2.2.1.6	4.5	-	pyruvate	-	395886	
2.2.1.6	4.58	-	pyruvate	wild-type, pH not specified in the publication, temperature not specified in the publication	719010	
2.2.1.6	4.7	-	pyruvate	mutant Q480W, pH 7.6, 37°C	719466	
2.2.1.6	4.7	-	pyruvate	mutant with a deleted C-terminal domain in the regulatory subunit IlvN, pH 7.3, 37°C	695780	
2.2.1.6	4.8	-	pyruvate	37°C, recombinant holoenzyme	672368	
2.2.1.6	4.8	-	pyruvate	pH 7.4, 37°C, recombinant enzyme	697061	
2.2.1.6	4.8	-	pyruvate	wild-type, pH 7.6, 37°C	719466	
2.2.1.6	4.94	-	pyruvate	mutant W516R, pH 7.5, 37°C	719375	
2.2.1.6	5	-	2-oxobutanoate	at 2 mM pyruvate	395917	
2.2.1.6	5	-	pyruvate	-	395872	
2.2.1.6	5	-	pyruvate	AHAS I	395871	
2.2.1.6	5	-	pyruvate	wild-type, 37°C, pH 7.6	657912	
2.2.1.6	5.2	-	pyruvate	wild-type, pH 7.6, 37°C	660390	
2.2.1.6	5.5	-	pyruvate	-	395910	
2.2.1.6	5.6	-	2-oxobutanoate	wild-type, pH 7.3, 37°C	695780	
2.2.1.6	5.7	-	pyruvate	mutant D374A, pH 7.5, 37°C	658223	
2.2.1.6	5.8	-	pyruvate	mutant R101A, pH 7.5, 37°C	719375	
2.2.1.6	6	-	pyruvate	wild-type with His-tag, pH 7.6, 37°C	660390	
2.2.1.6	6.04	-	pyruvate	mutant enzyme P126A, at pH 7.5 and 37°C	733745	
2.2.1.6	6.04	-	pyruvate	pH 7.5, 37°c, recombinant mutant P126A	733745	
2.2.1.6	6.53	-	pyruvate	pH 7.5, 37°C, recombinant wild-type enzyme	672868	
2.2.1.6	6.6	-	pyruvate	wild-type, pH 7.6, 37°C	718875, 718895	
2.2.1.6	6.8	-	pyruvate	-	395921	
2.2.1.6	6.9	-	2-oxobutanoate	mutant with a deleted C-terminal domain in the regulatory subunit IlvN, pH 7.3, 37°C	695780	
2.2.1.6	7	-	pyruvate	enzyme form II	395907	
2.2.1.6	7	-	pyruvate	mutant D428N, 37°C, pH 7.6	657912	
2.2.1.6	7	-	pyruvate	pH 7.6, isoenzyme AHAS III	395876	
2.2.1.6	7.1	-	pyruvate	mutant E47A, pH 7.6, 37°C	718895	
2.2.1.6	7.3	-	pyruvate	mutant E47Q, 37°C, pH 7.6	657912	
2.2.1.6	7.3	-	pyruvate	mutant V375I, pH 7.6, 37°C	718875	
2.2.1.6	7.6	-	pyruvate	isoenzyme III	395887	
2.2.1.6	7.7	-	pyruvate	mutant E47A, 37°C, pH 7.6	657912	
2.2.1.6	7.7	-	pyruvate	mutant E47Q, pH 7.6, 37°C	718895	
2.2.1.6	7.8	-	pyruvate	wild-type, pH 7.3, 37°C	695780	
2.2.1.6	7.82	-	pyruvate	mutant S539F, pH not specified in the publication, temperature not specified in the publication	719010	
2.2.1.6	8	-	pyruvate	30°C, 120 ng protein in assay	658566	
2.2.1.6	8	-	pyruvate	AHAS II	395871	
2.2.1.6	8	-	pyruvate	enzyme form I	395907	
2.2.1.6	8	-	pyruvate	mutant V477I, pH 7.6, 37°C	719466	
2.2.1.6	8.01	-	pyruvate	Km-value of the first active site	395902	
2.2.1.6	8.31	-	Hydroxypyruvate	-	659590	
2.2.1.6	8.36	-	pyruvate	mutant S506R, pH not specified in the publication, temperature not specified in the publication	719010	
2.2.1.6	8.5	-	pyruvate	wild type enzyme, at pH 7.5 and 20°C	758092	
2.2.1.6	8.7	-	pyruvate	mutant Q487A, pH 7.0, 37°C	695784	
2.2.1.6	8.8	-	pyruvate	holoenzyme, pH 8.0, 60°C	736351	
2.2.1.6	8.8	-	pyruvate	mutant enzyme S627N, at pH 7.5 and 20°C	758092	
2.2.1.6	8.9	-	pyruvate	mutant S27A, pH 7.5, 37°C	719375	
2.2.1.6	9	-	pyruvate	mutant enzyme S627N, in the presence of imazamox, at pH 7.5 and 20°C	758092	
2.2.1.6	9.1	-	2-oxobutanoate	mutant V375A, pH 7.6, 37°C	718875	
2.2.1.6	9.2	-	pyruvate	pH 7.5, 37°C, recombinant enzyme	695852	
2.2.1.6	10	-	pyruvate	-	395918	
2.2.1.6	10	-	pyruvate	30°C, 60 ng protein in assay	658566	
2.2.1.6	10	-	pyruvate	pH 7.0, 37°C, isoform ALS I	659100	
2.2.1.6	10.54	-	pyruvate	mutant S506A, pH not specified in the publication, temperature not specified in the publication	719010	
2.2.1.6	10.6	-	pyruvate	pH 7.6, isoenzyme AHAS II	395876	
2.2.1.6	11	-	pyruvate	pH 7.0, 37°C, isoform ALS II	659100	
2.2.1.6	11.2	-	pyruvate	mutant W464L with His-tag, pH 7.6, 37°C	660390	
2.2.1.6	11.4	-	pyruvate	mutant enzyme S627N, in the presence of imazethapyr, at pH 7.5 and 20°C	758092	
2.2.1.6	11.48	-	pyruvate	mutant S167R, pH not specified in the publication, temperature not specified in the publication	719010	
2.2.1.6	11.7	-	pyruvate	pH 7.5, wild-type enzyme	674354	
2.2.1.6	11.7	-	pyruvate	wild-type, pH 7.5, 37°C	658223	
2.2.1.6	11.9	-	pyruvate	pH 7.5, 37°C	720392	
2.2.1.6	12	-	pyruvate	large subunit, pH 8.0, 60°C	736351	
2.2.1.6	12.1	-	pyruvate	wild-type recombinant enzyme	395920	
2.2.1.6	13	-	pyruvate	-	395889	
2.2.1.6	13	-	pyruvate	mutant L476M, pH 7.6, 37°C	719466	
2.2.1.6	13.6	-	pyruvate	wild-type, pH 7.0, 37°C	695784	
2.2.1.6	13.68	-	pyruvate	mutant C607S, pH 7.5, 37°C	657718	
2.2.1.6	13.71	-	pyruvate	mutant H392M, 37°C, pH 7.5	657720	
2.2.1.6	13.8	-	pyruvate	mutant V375A, pH 7.6, 37°C	718875	
2.2.1.6	14.68	-	pyruvate	mutant enzyme Q112N, at pH 6.8 and 37°C	733987	
2.2.1.6	14.68	-	pyruvate	pH 7.5, 37°C, recombinant mutant Q112N	733987	
2.2.1.6	15.05	-	pyruvate	wild-type, pH 7.5, 37°C	657718	
2.2.1.6	16.09	-	pyruvate	wild-type, 37°C, pH 7.5	657720	
2.2.1.6	16.24	-	pyruvate	mutant enzyme E49Q, pH and temperature not specified in the publication	733597	
2.2.1.6	16.24	-	pyruvate	recombinant mutant E49Q, pH and temperature not specified in the publication	733597	
2.2.1.6	16.4	-	pyruvate	at pH 7.0 and 80°C	733241	
2.2.1.6	16.4	-	pyruvate	pH 7.0, 80°C	733241	
2.2.1.6	16.4	-	pyruvate	pH 7.0, 85°C, recombinant catalytic subunit	733241	
2.2.1.6	16.43	-	pyruvate	mutant enzyme Q411E, at pH 6.8 and 37°C	733987	
2.2.1.6	16.43	-	pyruvate	pH 7.5, 37°C, recombinant mutant Q411E	733987	
2.2.1.6	17	-	pyruvate	-	395896, 395914	
2.2.1.6	17.06	-	pyruvate	wild-type, pH 7.5, 37°C	657724	
2.2.1.6	17.3	-	pyruvate	mutant F109M, pH 7.6, 37°C	718875	
2.2.1.6	17.5	-	pyruvate	pH 7.5, 37°C	719329	
2.2.1.6	17.9	-	pyruvate	mutant V391A, pH 7.6, 37°C	719466	
2.2.1.6	19.78	-	pyruvate	mutant enzyme Q112E, at pH 6.8 and 37°C	733987	
2.2.1.6	19.78	-	pyruvate	pH 7.5, 37°C, recombinant mutant Q112E	733987	
2.2.1.6	20	-	pyruvate	37°C	660559	
2.2.1.6	21	-	pyruvate	mutant E60Q, pH 7.6, 37°C	719466	
2.2.1.6	21.55	-	pyruvate	mutant C411S, pH 7.5, 37°C	657718	
2.2.1.6	22.6	-	pyruvate	-	395912	
2.2.1.6	24.6	-	pyruvate	mutant enzyme H111R, at pH 6.8 and 37°C	733987	
2.2.1.6	24.6	-	pyruvate	pH 7.5, 37°C, recombinant mutant H111R	733987	
2.2.1.6	25.5	-	pyruvate	pH 7.5, 37°C, in the presence of 3.5 M KCl	727336	
2.2.1.6	26	-	pyruvate	mutant M263A, pH 7.6, 37°C	719466	
2.2.1.6	26.23	-	pyruvate	mutant K299Q pH 7.5, 37°C	657724	
2.2.1.6	28	-	pyruvate	mutant E60A, pH 7.6, 37°C	719466	
2.2.1.6	29.5	-	pyruvate	mutant Q110N, pH 7.6, 37°C	718895	
2.2.1.6	30.2	-	pyruvate	mutant Q110A, pH 7.6, 37°C	718895	
2.2.1.6	30.58	-	pyruvate	mutant enzyme E49A, pH and temperature not specified in the publication	733597	
2.2.1.6	30.58	-	pyruvate	recombinant mutant E49A, pH and temperature not specified in the publication	733597	
2.2.1.6	31.2	-	pyruvate	mutant enzyme Q112V, at pH 6.8 and 37°C	733987	
2.2.1.6	31.2	-	pyruvate	pH 7.5, 37°C, recombinant mutant Q112V	733987	
2.2.1.6	36	-	pyruvate	mutant D428E, 37°C, pH 7.6	657912	
2.2.1.6	36	-	pyruvate	mutant M250A with His-tag, pH 7.6, 37°C	660390	
2.2.1.6	36.6	-	pyruvate	mutant enzyme H111F, at pH 6.8 and 37°C	733987	
2.2.1.6	36.6	-	pyruvate	pH 7.5, 37°C, recombinant mutant H111F	733987	
2.2.1.6	38	-	pyruvate	mutant R276K with His-tag, pH 7.6, 37°C	660390	
2.2.1.6	40.4	-	pyruvate	mutant Q110H pH 7.6, 37°C	718895	
2.2.1.6	50	-	pyruvate	-	395922	
2.2.1.6	50	-	pyruvate	mutant R289K, pH 7.6, 37°C	719466	
2.2.1.6	50	-	pyruvate	wild-type, pH 7.3, 37°C, presence of 10 mM L-valine	695780	
2.2.1.6	54	-	pyruvate	wild-type, pH 7.3, 37°C, presence of 10 mM L-isoleucine	695780	
2.2.1.6	55.8	-	pyruvate	mutant D374A/D375A, pH 7.5, 37°C	658223	
2.2.1.6	65	-	pyruvate	wild-type, pH 7.3, 37°C, presence of 10 mM L-leucine	695780	
2.2.1.6	70	-	pyruvate	pH 6.5, 37°C	657572	
2.2.1.6	76.63	-	pyruvate	mutantH351F, 37°C, pH 7.5	657720	
2.2.1.6	100	-	pyruvate	Km-value of the second active site	395902	
2.2.1.6	104	-	pyruvate	mutant with a deleted C-terminal domain in the regulatory subunit IlvN, pH 7.3, 37°C, presence of 10 mM L-isoleucine	695780	
2.2.1.6	104	-	pyruvate	mutant with a deleted C-terminal domain in the regulatory subunit IlvN, pH 7.3, 37°C, presence of 10 mM L-leucine	695780	
2.2.1.6	104	-	pyruvate	mutant with a deleted C-terminal domain in the regulatory subunit IlvN, pH 7.3, 37°C, presence of 10 mM L-valine	695780	
2.2.1.6	109.3	-	pyruvate	mutant D375A, pH 7.5, 37°C	658223	
2.2.1.6	109.4	-	pyruvate	mutant K255F, pH 7.5, 37°C	657724	
2.2.1.6	110.6	-	pyruvate	mutant Q110E, pH 7.6, 37°C	718895	
2.2.1.6	113.9	-	pyruvate	pH 7.5, mutant R141K	674354	
2.2.1.6	115.5	-	pyruvate	mutant K255Q, pH 7.5, 37°C	657724	
2.2.1.6	116.8	-	pyruvate	pH 7.5, mutant R141F	674354	
2.2.1.6	124	-	pyruvate	mutant R289Q, pH 7.6, 37°C	719466	
2.2.1.6	148	-	pyruvate	pH 7.5, 37°C, recombinant mutant W573F	672868	
2.2.1.6	167.4	-	pyruvate	pH 7.5, mutant R372F	674354	
2.2.1.6	266.7	-	pyruvate	mutant D374E/D375E, pH 7.5, 37°C	658223	
2.2.1.6	287.8	-	pyruvate	mutant H351M, 37°C, pH 7.5	657720	
2.2.1.6	300	-	2-oxobutanoate	wild-type, pH 7.6, 37°C	718875	
2.2.1.6	337.3	-	pyruvate	pH 7.5, mutant R376K	674354	
2.2.1.6	466.7	-	pyruvate	mutant enzyme P126V, at pH 7.5 and 37°C	733745	
2.2.1.6	466.7	-	pyruvate	pH 7.5, 37°c, recombinant mutant P126V	733745	
2.2.1.6	475.9	-	pyruvate	pH 7.5, mutant R372K	674354	
2.2.1.6	557.6	-	pyruvate	mutant D374E, pH 7.5, 37°C	658223	
2.2.1.6	807.7	-	pyruvate	mutant enzyme P126T, at pH 7.5 and 37°C	733745	
2.2.1.6	807.7	-	pyruvate	pH 7.5, 37°c, recombinant mutant P126T	733745	
2.2.1.6	959.5	-	pyruvate	mutant H351Q, 37°C, pH 7.5	657720