1.5.1.2 additional information - additional information - 392131, 392133, 392136, 392138, 392148 1.5.1.2 additional information - additional information steady-state kinetics of the wild-type enzyme and mutants 762678 1.5.1.2 0.006 - NADPH - 392112 1.5.1.2 0.0071 - NADPH - 392117 1.5.1.2 0.012 - NADPH apparent value, at pH 7.5 and 37°C 723922 1.5.1.2 0.0128 - NADPH pH 7, 28°C 762709 1.5.1.2 0.02 - 1-pyrroline-5-carboxylate cosubstrate NADPH, pH 7.5, 35°C 740491 1.5.1.2 0.02 - NADPH - 390310 1.5.1.2 0.022 - 1-pyrroline-5-carboxylate cosubstrate NADPH 392112 1.5.1.2 0.024 0.025 1-pyrroline-5-carboxylate - 392111 1.5.1.2 0.025 - NADH - 392146 1.5.1.2 0.026 - NADPH pH 7.8, 35°C 741069 1.5.1.2 0.0277 - 1-pyrroline-5-carboxylate pH 7, 28°C 762709 1.5.1.2 0.03 - NADH mutant R119C, pH 7.5, 37°C 762678 1.5.1.2 0.03 - NADPH - 392140 1.5.1.2 0.034 - NADH pH 7.5, 37°C, recombinant mutant R119C, with 1-pyrroline-5-carboxylate 762678 1.5.1.2 0.039 - NADPH pH 7.5, 35°C 740491 1.5.1.2 0.04 - 1-pyrroline-5-carboxylate cofactor NADH 676952 1.5.1.2 0.047 - 1-pyrroline-5-carboxylate cofactor NADPH 676952 1.5.1.2 0.048 - NADH - 392119 1.5.1.2 0.05 - NADH - 390310 1.5.1.2 0.05 - NADPH - 392125 1.5.1.2 0.051 - DL-pyrroline-5-carboxylate cosubstrate NADPH 392112 1.5.1.2 0.056 - NADPH - 392119 1.5.1.2 0.057 - NADH - 676952 1.5.1.2 0.059 - NADPH - 676952 1.5.1.2 0.06 - NADH - 392130 1.5.1.2 0.06 - NADPH - 392110 1.5.1.2 0.07 - NADH wild-type, pH 7.5, 37°C 740763 1.5.1.2 0.08 - DL-pyrroline-5-carboxylate - 392119 1.5.1.2 0.088 - NADH pH 7.5, 30°C 763322 1.5.1.2 0.09 - 1-pyrroline-5-carboxylate - 392130 1.5.1.2 0.09 - 1-pyrroline-5-carboxylate with NADH 390310 1.5.1.2 0.093 - NADP+ - 676952 1.5.1.2 0.096 - 1-pyrroline-5-carboxylate cofactor NADPH, pH 7.8, 35°C 741069 1.5.1.2 0.1 - NADH - 392108, 392125 1.5.1.2 0.1 - NADPH etiolated shoots 392107 1.5.1.2 0.119 - NADPH pH 7.5, 37°C, recombinant mutant R251C, with 1-pyrroline-5-carboxylate 762678 1.5.1.2 0.12 - 1-pyrroline-5-carboxylate with NADPH 390310, 392110 1.5.1.2 0.12 - L-proline - 676952 1.5.1.2 0.12 - NADPH chloroplast 392107 1.5.1.2 0.12 - NADPH mutant R251C, pH 7.5, 37°C 762678 1.5.1.2 0.125 - 1-pyrroline-5-carboxylate - 392117 1.5.1.2 0.135 - NADH pH 7.5, 30°C 763462 1.5.1.2 0.14 - 1-pyrroline-5-carboxylate with NADH 392126 1.5.1.2 0.141 - 3,4-dehydro-L-proline - 676969 1.5.1.2 0.15 - 1-pyrroline-5-carboxylate with NADPH 392126 1.5.1.2 0.151 - NAD+ wild type enzyme, 1 mM fixed thioproline, thioproline dehydrogenase activity assay 675382 1.5.1.2 0.154 - 1-pyrroline-5-carboxylate - 392125 1.5.1.2 0.159 - NADPH mutant T238A, pH 7.5, 37°C 740763 1.5.1.2 0.17 - 1-pyrroline-5-carboxylate - 392108 1.5.1.2 0.176 - NADH apparent value, at pH 7.5 and 37°C 723922 1.5.1.2 0.184 - 1-pyrroline-5-carboxylate apparent value, with NADPH as cosubstrate, at pH 7.5 and 37°C 723922 1.5.1.2 0.19 - 1-pyrroline-5-carboxylate - 392146 1.5.1.2 0.19 - NADH chloroplast 392107 1.5.1.2 0.2 - 1-pyrroline-5-carboxylate with NADH 392110 1.5.1.2 0.2 - NADH - 392117 1.5.1.2 0.21 - 1-pyrroline-5-carboxylate cosubstrate NADH 392112 1.5.1.2 0.21 - NADH pH 7.8, 35°C 741069 1.5.1.2 0.216 - NADPH pH 7.5, 37°C, recombinant wild-type enzyme, with 1-pyrroline-5-carboxylate 762678 1.5.1.2 0.22 - NADPH wild-type, pH 7.5, 37°C 762678 1.5.1.2 0.223 - NADPH pH 7.5, 30°C 763322 1.5.1.2 0.23 - NADH - 392140 1.5.1.2 0.23 - NADH NADH 392126 1.5.1.2 0.235 - NAD+ E221A mutant, 1 mM fixed thioproline, thioproline dehydrogenase activity assay 675382 1.5.1.2 0.24 - 1-pyrroline-5-carboxylate cosubstrate NADH, pH 7.5, 35°C 740491 1.5.1.2 0.273 - 1-pyrroline-5-carboxylate cosubstrate NADH, pH 7.5, 30°C 763462 1.5.1.2 0.283 - NADPH wild-type, pH 7.5, 37°C 740763 1.5.1.2 0.298 - NADH pH 7.5, 37°C, recombinant wild-type enzyme, with 1-pyrroline-5-carboxylate 762678 1.5.1.2 0.3 - NADH wild-type, pH 7.5, 37°C 762678 1.5.1.2 0.317 - 1-pyrroline-5-carboxylate pH 7.5, 37°C, recombinant mutant R119C, with NADH 762678 1.5.1.2 0.32 - 1-pyrroline-5-carboxylate mutant R119C, cosubstrate NADH, pH 7.5, 37°C 762678 1.5.1.2 0.33 - 1-pyrroline-5-carboxylate mutant R119C, cosubstrate NADPH, pH 7.5, 37°C 762678 1.5.1.2 0.33 - L-pyrroline-5-carboxylate pH 6.5 392123 1.5.1.2 0.33 - L-pyrroline-5-carboxylate pH 8.0 392123 1.5.1.2 0.334 - 1-pyrroline-5-carboxylate pH 7.5, 37°C, recombinant mutant R119C, with NADPH 762678 1.5.1.2 0.34 - DL-pyrroline-5-carboxylate - 392140 1.5.1.2 0.349 - 1-pyrroline-5-carboxylate apparent value, with NADH as cosubstrate, at pH 7.5 and 37°C 723922 1.5.1.2 0.37 - 1-pyrroline-5-carboxylate with NADPH 392138 1.5.1.2 0.37 - NADH - 392112 1.5.1.2 0.43 - NADH etiolated shoots 392107 1.5.1.2 0.44 - 1-pyrroline-5-carboxylate - 392128 1.5.1.2 0.45 - 1-pyrroline-5-carboxylate with NADPH 392133 1.5.1.2 0.468 - NADH pH 7.5, 35°C 740491 1.5.1.2 0.48 - NADP+ E221A mutant, 1 mM fixed thioproline, thioproline dehydrogenase activity assay 675382 1.5.1.2 0.53 - 1-pyrroline-5-carboxylate with NADH 392133 1.5.1.2 0.537 - NADPH pH 7.5, 37°C, recombinant mutant R119C, with 1-pyrroline-5-carboxylate 762678 1.5.1.2 0.54 - NADPH mutant R119C, pH 7.5, 37°C 762678 1.5.1.2 0.58 - NADH - 392047 1.5.1.2 0.62 - DL-pyrroline-5-carboxylate - 392047 1.5.1.2 0.62 - DL-pyrroline-5-carboxylate cosubstrate NADH 392111, 392112 1.5.1.2 0.667 - 1-pyrroline-5-carboxylate cosubstrate NADPH, wild-type, pH 7.5, 37°C 740763 1.5.1.2 0.719 - NADPH pH 7.5, 30°C 763462 1.5.1.2 0.73 - NADP+ E221A mutant, fixed 1 mM NADP+ 675382 1.5.1.2 0.915 - 1-pyrroline-5-carboxylate cofactor NADH, pH 7.8, 35°C 741069 1.5.1.2 0.949 - 1-pyrroline-5-carboxylate pH 7.5, 37°C, recombinant wild-type enzyme, with NADPH 762678 1.5.1.2 0.95 - NADH mutant R251C, pH 7.5, 37°C 762678 1.5.1.2 0.953 - NADH pH 7.5, 37°C, recombinant mutant R251C, with 1-pyrroline-5-carboxylate 762678 1.5.1.2 0.99 - 1-pyrroline-5-carboxylate wild-type, cosubstrate NADPH, pH 7.5, 37°C 762678 1.5.1.2 1 1.2 NAD+ - 392123 1.5.1.2 1.053 - 1-pyrroline-5-carboxylate cosubstrate NADPH, pH 7.5, 30°C 763462 1.5.1.2 1.25 - L-proline pH 10.2 392123 1.5.1.2 1.26 - NADP+ wild type enzyme, fixed 1 mM NADP+ 675382 1.5.1.2 1.315 - 1-pyrroline-5-carboxylate pH 7.5, 37°C, recombinant mutant R251C, with NADH 762678 1.5.1.2 1.32 - 1-pyrroline-5-carboxylate mutant R251C, cosubstrate NADH, pH 7.5, 37°C 762678 1.5.1.2 1.499 - 1-pyrroline-5-carboxylate pH 7.5, 37°C, recombinant mutant R251C, with NADPH 762678 1.5.1.2 1.5 - 1-pyrroline-5-carboxylate mutant R251C, cosubstrate NADPH, pH 7.5, 37°C 762678 1.5.1.2 1.509 - 1-pyrroline-5-carboxylate pH 7.5, 37°C, recombinant wild-type enzyme, with NADH 762678 1.5.1.2 1.51 - 1-pyrroline-5-carboxylate wild-type, cosubstrate NADH, pH 7.5, 37°C 762678 1.5.1.2 1.55 - L-proline L-proline oxidation 392146 1.5.1.2 1.55 - NADH - 392110 1.5.1.2 1.62 1.64 1-pyrroline-5-carboxylate - 392111 1.5.1.2 2.887 - 1-pyrroline-5-carboxylate mutant T238A, wild-type, pH 7.5, 37°C 740763 1.5.1.2 3.06 - NADP+ wild type enzyme, 1 mM fixed thioproline, thioproline dehydrogenase activity assay 675382 1.5.1.2 4.4 - DELTA1-pyrroline-3-hydroxy-5-carboxylate - 392132 1.5.1.2 10.5 - NAD+ L-proline oxidation 392146 1.5.1.2 31 - L-proline pH 8.0 392123 1.5.1.2 50 - L-proline - 392144