1.4.1.13 additional information - additional information steady-state kinetic analysis, overview 725886 1.4.1.13 0.0022 - NADPH in the presence of 1 mM alpha-ketoglutarate and 5 mM L-glutamine 391442 1.4.1.13 0.003 - NADPH - 391455 1.4.1.13 0.0035 - NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: iodonitrotetrazolium 391463 1.4.1.13 0.0047 - alpha-ketoglutarate in the presence of 0.04 mM NADPH and 5 mM L-glutamine 391442 1.4.1.13 0.006 - NADPH in the presence of a fixed concentration of L-glutamine, and varied concentrations of alpha-ketoglutarate 391459 1.4.1.13 0.00625 - NADPH - 391446 1.4.1.13 0.007 - alpha-ketoglutarate in the presence of a fixed concentration of NADPH, and varied concentrations of L-glutamine 391459 1.4.1.13 0.007 - NADPH in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of L-glutamine 391459 1.4.1.13 0.0071 - NADPH - 391454 1.4.1.13 0.0073 - alpha-ketoglutarate - 391438 1.4.1.13 0.0077 - NADPH - 391438 1.4.1.13 0.0086 - alpha-ketoglutarate - 391448 1.4.1.13 0.009 - 2-oxoglutarate - 391454 1.4.1.13 0.0098 - NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: menadione 391463 1.4.1.13 0.01 - 2-oxoglutarate - 391462 1.4.1.13 0.01 - alpha-ketoglutarate in the presence of a fixed concentration of NADH, and varied concentrations of L-glutamine 391459 1.4.1.13 0.01 - thio-NADPH in the presence of 2.5 mM 2-oxo-glutarate and 5 mM L-glutamine, the apparent maximal velocity is 54% that obtained in the presence of NADPH 391445 1.4.1.13 0.0118 - NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: ferricyanide 391463 1.4.1.13 0.012 - alpha-ketoglutarate in the presence of a fixed concentration of L-glutamine, and varied concentrations of NADH 391459 1.4.1.13 0.012 - NADPH - 391460 1.4.1.13 0.013 - alpha-ketoglutarate in the presence of a fixed concentration of L-glutamine, and varied concentrations of NADPH 391459 1.4.1.13 0.013 - NADPH - 391449 1.4.1.13 0.014 - NADPH - 391448 1.4.1.13 0.014 - NADPH NH3-dependent glutamate synthase and apoglutamate synthase 391456 1.4.1.13 0.015 - 2-oxoglutarate - 391451 1.4.1.13 0.015 - NADPH - 391452 1.4.1.13 0.016 - NADPH - 391462 1.4.1.13 0.017 - acetylpyridine-NADPH in the presence of 2.5 mM 2-oxo-glutarate and 5 mM L-glutamine, the apparent maximal velocity is 3.7% that obtained in the presence of NADPH 391445 1.4.1.13 0.022 - NADPH - 391451 1.4.1.13 0.029 - 2-oxoglutarate - 391446 1.4.1.13 0.029 - L-glutamine - 391451 1.4.1.13 0.035 - 2-oxoglutarate - 391452 1.4.1.13 0.035 - menadione of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme 391463 1.4.1.13 0.035 - NADPH - 391461 1.4.1.13 0.042 - NADPH pH 7.5, 37°C, pGLTY2 725886 1.4.1.13 0.05 - alpha-ketoglutarate - 391455 1.4.1.13 0.05 - iodonitrotetrazolium of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme 391463 1.4.1.13 0.053 - alpha-ketoglutarate - 391440 1.4.1.13 0.06 - alpha-ketoglutarate - 391447 1.4.1.13 0.06 - NADPH - 391447 1.4.1.13 0.065 - L-glutamine - 391462 1.4.1.13 0.077 - L-glutamine - 391440 1.4.1.13 0.084 - NADPH of NADPH: iodonitrotetrazolium oxidoreductase activity of the G298A-beta subunit 391466 1.4.1.13 0.091 - NADH in the presence of a fixed concentration of L-glutamine, and varied concentrations of alpha-ketoglutarate 391459 1.4.1.13 0.102 - L-glutamine in the presence of a fixed concentration of NADPH, and varied concentrations of alpha-ketoglutarate 391459 1.4.1.13 0.108 - L-glutamine in the presence of a fixed concentration of NADH, and varied concentrations of alpha-ketoglutarate 391459 1.4.1.13 0.11 - NADPH - 391440 1.4.1.13 0.11 - NADPH pH 7.5, 70°C, pGLTY2 725886 1.4.1.13 0.113 - NADH in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of L-glutamine 391459 1.4.1.13 0.115 - L-glutamine in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of NADPH 391459 1.4.1.13 0.12 - L-glutamine in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of NADH 391459 1.4.1.13 0.13 - L-glutamine - 391452 1.4.1.13 0.14 - ferricyanide of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme 391463 1.4.1.13 0.175 - NADPH dimeric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 10 mM L-glutamine, and 5 mM 2-oxoglutarate 687801 1.4.1.13 0.2 - L-glutamine - 391454 1.4.1.13 0.23 - 2-oxoglutarate dimeric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 10 mM L-glutamine, and 0.1 mM NADPH 687801 1.4.1.13 0.23 - L-glutamine holoenzyme, buffer: Hepes, pH 8.5 or 7.5 391469 1.4.1.13 0.24 - 2-oxoglutarate NH3-dependent glutamate synthase and apoglutamate synthase 391456 1.4.1.13 0.24 - L-glutamine - 391447 1.4.1.13 0.245 - NADPH hexameric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 10 mM L-glutamine, and 5 mM 2-oxoglutarate 687801 1.4.1.13 0.25 - L-glutamine - 391438 1.4.1.13 0.3 - alpha-ketoglutarate - 391460 1.4.1.13 0.3 - L-glutamine - 391460 1.4.1.13 0.35 - alpha-ketoglutarate - 391461 1.4.1.13 0.45 - L-glutamine - 391446 1.4.1.13 0.5 2 NH4Cl - 391460 1.4.1.13 0.59 - L-glutamine - 391448 1.4.1.13 0.63 - L-glutamine alpha subunit, buffer: Hepes, pH 7.5 391469 1.4.1.13 0.69 - L-glutamine dimeric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 2.5 mM 2-oxoglutarate, and 0.1 mM NADPH 687801 1.4.1.13 0.73 - L-glutamine holoenzyme, buffer: CAPS, pH 9.5 391469 1.4.1.13 0.75 - 2-oxoglutarate hexameric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 10 mM L-glutamine, and 0.1 mM NADPH 687801 1.4.1.13 0.92 - L-glutamine alpha subunit, buffer: Hepes, pH 8.5 391469 1.4.1.13 1.1 - L-glutamine - 391455 1.4.1.13 1.5 - L-glutamine alpha subunit, buffer: CAPS, pH 9.5 391469 1.4.1.13 1.7 - L-glutamine - 391443 1.4.1.13 2 - 2-oxoglutarate - 391443 1.4.1.13 2.1 - L-glutamine hexameric enzyme, at 25°C in 50 mM HEPES/KOH buffer, pH 7.5, 1 M NaCl, in the presence of 2.5 mM 2-oxoglutarate, and 0.1 mM NADPH 687801 1.4.1.13 2.6 - L-glutamine - 391461 1.4.1.13 6.42 - NADPH pH 7.5, 37°C, pGLTZ 725886 1.4.1.13 16.3 - NADPH pH 7.5, 70°C, pGLTZ 725886 1.4.1.13 22 - NH4Cl - 391454 1.4.1.13 44 - NH4Cl - 391451