1.3.98.1 additional information - additional information 10% v/v dimethyl sulfoxide and 0.5% v/vTriton X-100, which seem to facilitate the substrate binding process with a small decrease in Km, enzyme-substrate complex, pseudo-first-order kinetics, thermodynamics, overview 710846 1.3.98.1 0.001 - S-dihydroorotate pH 9.0 390910 1.3.98.1 0.001 0.002 ferricyanide pH 6.0 390912 1.3.98.1 0.003 - S-dihydroorotate with O2 390927 1.3.98.1 0.005 - S-dihydroorotate pH 7.4 390910 1.3.98.1 0.0084 0.045 S-dihydroorotate with fumarate 390927 1.3.98.1 0.00856 - (S)-dihydroorotate assay based on isothermal titration calorimetry, pH 8.0, 25°C 710846 1.3.98.1 0.0086 - L-dihydroorotate pH not specified in the publication, temperature not specified in the publication 710846 1.3.98.1 0.0131 - 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone at saturating concentration of dihydroorotate 671050 1.3.98.1 0.014 - S-dihydroorotate - 689056 1.3.98.1 0.016 - CoQ pH 8.0, 22°C, wild-type 656285 1.3.98.1 0.016 - L-dihydroorotate in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C 686821 1.3.98.1 0.017 - S-dihydroorotate pH 9.0 390912 1.3.98.1 0.0192 - dihydroorotate in the presence of 0.125 mM fumarate 676964 1.3.98.1 0.024 - (S)-dihydroorotate recombinant enzyme, pH 7.5, 25°C 656747 1.3.98.1 0.0243 - dihydroorotate DHOD at 25°C 676289 1.3.98.1 0.0259 - dihydroorotate native DHOD at 25°C 676289 1.3.98.1 0.026 - (S)-dihydroorotate native enzyme, pH 7.5, 25°C 656747 1.3.98.1 0.03 - dihydroorotate 37°C, pH 7.0 676289 1.3.98.1 0.03 - dihydroorotate DHOD1 and DHOD2 at 37°C 676289 1.3.98.1 0.03 - fumarate 37°C, pH 7.0 676289 1.3.98.1 0.03 - fumarate DHOD1 at 37°C 676289 1.3.98.1 0.032 - CoQ pH 8.0, 22°C, mutant R265A 656285 1.3.98.1 0.0332 - dihydroorotate in the presence of 0.25 mM fumarate 676964 1.3.98.1 0.035 - fumarate 37°C, pH 7.0 676289 1.3.98.1 0.035 - fumarate DHOD2 at 37°C 676289 1.3.98.1 0.0439 - fumarate DHOD at 25°C 676289 1.3.98.1 0.044 - fumarate recombinant enzyme, pH 7.5, 25°C 656747 1.3.98.1 0.0442 - dihydroorotate pH 8.5, 70°C 655517 1.3.98.1 0.0472 - dihydroorotate in the presence of 0.5 mM fumarate 676964 1.3.98.1 0.053 - fumarate native enzyme, pH 7.5, 25°C 656747 1.3.98.1 0.0534 - fumarate native DHOD at 25°C 676289 1.3.98.1 0.0579 - dihydroorotate in the presence of 1 mM fumarate 676964 1.3.98.1 0.066 - CoQ pH 8.0, 22°C, mutant H185A 656285 1.3.98.1 0.067 - fumarate 37°C, pH 7.0 676289 1.3.98.1 0.067 - fumarate DHOD3 at 37°C 676289 1.3.98.1 0.071 - dihydroorotate 37°C, pH 7.0 676289 1.3.98.1 0.071 - dihydroorotate DHOD3 at 37°C 676289 1.3.98.1 0.0718 - fumarate in the presence of 0.02 mM dihydroorotate 676964 1.3.98.1 0.08 - fumarate - 689056 1.3.98.1 0.091 - L-dihydroorotate pH 8.0, 22°C, wild-type 656285 1.3.98.1 0.0975 - fumarate in the presence of 0.03 mM dihydroorotate 676964 1.3.98.1 0.105 - L-dihydroorotate pH 8.0, 22°C, mutant R265A 656285 1.3.98.1 0.115 - 2,6-dichloroindophenol in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C 686821 1.3.98.1 0.12 - fumarate assay based on isothermal titration calorimetry, pH 8.0, 25°C 710846 1.3.98.1 0.12 - fumarate pH not specified in the publication, temperature not specified in the publication 710846 1.3.98.1 0.123 - L-dihydroorotate pH 8.0, 22°C, mutant H185A 656285 1.3.98.1 0.1282 - fumarate in the presence of 0.04 mM dihydroorotate 676964 1.3.98.1 0.344 - ferricyanide pH 8.5, 70°C 655517 1.3.98.1 0.4135 - fumarate in the presence of 0.05 mM dihydroorotate 676964 1.3.98.1 0.5 - S-dihydroorotate with ferricyanide as electron acceptor 390909