1.2.4.1 additional information - additional information detailed kinetic and thermodynamic analysis 672086 1.2.4.1 additional information - additional information kinetics 655474 1.2.4.1 additional information - additional information sigmoidal dependence on the concentration of pyruvate, Hill-coefficient: 1.85 348960 1.2.4.1 additional information - additional information with aging of rats the Vmax of the enzyme complex increases by 60%, while the Km decreases by 1.6fold in the same time, enzyme expression level remaines constant, more efficient catabolization of pyruvate due to adaptive phosphorylation of the enzyme within the multienzyme complex, age-related decline in pyruvate dehydrogenase kinase PDK-4 654462 1.2.4.1 0.00056 - thiamine diphosphate mutant R267A, pH 7.0, 25°C 655474 1.2.4.1 0.0009 - thiamine diphosphate recombinant enzyme 348987 1.2.4.1 0.0011 - pyruvate wild-type enzyme, pH 7.0, 25°C 655474 1.2.4.1 0.0011 - pyruvate wild-type, pH 7.0, 30°C 735664 1.2.4.1 0.0012 - pyruvate mutant F266A, pH 7.0, 25°C 655474 1.2.4.1 0.0015 - thiamine diphosphate recombinant enzyme 348987 1.2.4.1 0.00158 - thiamine diphosphate wild-type enzyme 348995 1.2.4.1 0.0021 - pyruvate mutant S283C, pH 7.0, 25°C 655474 1.2.4.1 0.0027 - thiamine diphosphate mutant Y281S/R282S, pH 7.0, 25°C 655474 1.2.4.1 0.0029 - thiamine diphosphate mutant Y281A/R282A/S283A, pH 7.0, 25°C 655474 1.2.4.1 0.003 - thiamine diphosphate mutant Y281A/R282A, pH 7.0, 25°C 655474 1.2.4.1 0.0052 - thiamine diphosphate mutant R282A, pH 7.0, 25°C 655474 1.2.4.1 0.0055 - thiamine diphosphate mutant Y281A, pH 7.0, 25°C 655474 1.2.4.1 0.00665 - thiamine diphosphate Y177A mutant enzyme 348995 1.2.4.1 0.0088 - pyruvate mutant R282A, pH 7.0, 25°C 655474 1.2.4.1 0.01 - pyruvate pH 7.6, 30°C, presence of 0.3 M KCl 672086 1.2.4.1 0.011 - pyruvate mutant D276A, pH 7.0, 25°C 655474 1.2.4.1 0.0124 - pyruvate pH 7.2, 37°C 762797 1.2.4.1 0.013 - thiamine diphosphate mutant S283C, pH 7.0, 25°C 655474 1.2.4.1 0.016 - 2-oxobutanoate pH 6.7, 30°C 736649 1.2.4.1 0.017 - pyruvate similar values 348955 1.2.4.1 0.0204 - pyruvate recombinant enzyme 348987 1.2.4.1 0.021 - thiamine diphosphate mutants F266A and D276A, pH 7.0, 25°C 655474 1.2.4.1 0.023 - thiamine diphosphate wild-type enzyme, pH 7.0, 25°C 655474 1.2.4.1 0.024 - lipoyl domain acyl group transfer reaction, recombinant pyruvate dehydrogenase with N-terminal deletion 348990 1.2.4.1 0.026 - pyruvate recombinant enzyme 348987 1.2.4.1 0.027 - lipoyl domain acyl group transfer reaction, recombinant wild-type pyruvate dehydrogenase 348990 1.2.4.1 0.049 - pyruvate mutant Y281A/R282A/S283A, pH 7.0, 25°C 655474 1.2.4.1 0.056 - pyruvate mutant Y281A/R282A, pH 7.0, 25°C 655474 1.2.4.1 0.056 - pyruvate presence of 0.002 mM inhibitor N-((1-((4-amino-2-methylpyrimidin-5-yl)methyl)-5-iodo-1H-1,2,3-triazol-4-yl)methyl)-4-nitrobenzenesulfonamide, pH 7.2, 37°C 762797 1.2.4.1 0.063 - pyruvate mutant Y281A, pH 7.0, 25°C 655474 1.2.4.1 0.065 - thiamine diphosphate in the presence of Ca2+ and Mg2+ 348974 1.2.4.1 0.066 - pyruvate mutant Y281S/R282S, pH 7.0, 25°C 655474 1.2.4.1 0.1304 - pyruvate mutant enzyme Q408A, with 2,6-dichlorophenolindophenol, at 30°C 687613 1.2.4.1 0.14 - pyruvate in the presence of Ca2+ and Mg2+ 348974 1.2.4.1 0.147 - pyruvate pH 6.7, 30°C 736649 1.2.4.1 0.149 - 2-oxovalerate pH 6.7, 30°C 736649 1.2.4.1 0.1714 - pyruvate mutant enzyme K410A, with 2,6-dichlorophenolindophenol, at 30°C 687613 1.2.4.1 0.2012 - pyruvate mutant enzyme K411E, with 2,6-dichlorophenolindophenol, at 30°C 687613 1.2.4.1 0.2336 - pyruvate mutant enzyme K411A, with 2,6-dichlorophenolindophenol, at 30°C 687613 1.2.4.1 0.25 - pyruvate - 348951 1.2.4.1 0.2609 - pyruvate wild type enzyme, with 2,6-dichlorophenolindophenol, at 30°C 687613 1.2.4.1 0.28 - pyruvate Y177A mutant enzyme 348995 1.2.4.1 0.291 - pyruvate mutant R267A, pH 7.0, 25°C 655474 1.2.4.1 0.442 - pyruvate mutant H271A, pH 7.0, 30°C 735664 1.2.4.1 0.515 - pyruvate wild-type enzyme 348995 1.2.4.1 0.531 - pyruvate Y177F mutant enzyme 348995 1.2.4.1 0.6286 - pyruvate mutant enzyme E401A, with 2,6-dichlorophenolindophenol, at 30°C 687613 1.2.4.1 0.7008 - pyruvate mutant enzyme K403A, with 2,6-dichlorophenolindophenol, at 30°C 687613 1.2.4.1 0.73 - E2p lipoyl domain wild type enzyme, at pH 7.0 and 30°C 725205 1.2.4.1 1 - Mg2+ - 348936 1.2.4.1 1.7 - pyruvate 30°C, cell extract 674246