1.17.1.9 additional information - additional information - 288115 1.17.1.9 additional information - additional information effect of organic solvents of formate dehydrogenase 288091 1.17.1.9 additional information - additional information the enzyme has two sites with about equal catalytic activity. The Km-values for formate are different for the two catalytic sites: 1.67 mM and 6.25 mM 288112 1.17.1.9 0.003 - NADH at pH 6.0 and 35°C 743715 1.17.1.9 0.0033 - formate pH 7.5, 30°C, heated DE-52-fraction 656618 1.17.1.9 0.0057 - NAD+ - 288113 1.17.1.9 0.0086 - NAD+ mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.0091 - NAD+ mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.0109 - NAD+ mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.011 - formate pH 7.5, 30°C, enzyme expressed in tobacco 656618 1.17.1.9 0.011 - formate pH 7.5, 30°C, enzyme from Sephadex G-15 desalted fraction 656618 1.17.1.9 0.011 - formate pH 7.5, 30°C, mitochondrial enzyme 656618 1.17.1.9 0.0117 - NAD+ mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.012 - formate pH 7.5, 30°C, enzyme from DE-52 chromatography 656618 1.17.1.9 0.0128 - NAD+ mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.0133 - NAD+ wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.0137 - NAD+ mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.014 - NAD+ mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.0143 - NAD+ mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.015 - NAD+ wild type enzyme, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 0.015 - NAD+ wild type enzyme, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 0.0157 - CO2 at pH 7.0 and 22°C 740581 1.17.1.9 0.017 - reduced 1,1'-diaminoethyl-4,4'-bipyridinium salt at pH 7.4, temperature not specified in the publication 740320 1.17.1.9 0.021 - NAD+ at pH 7.5 and 25°C 742752 1.17.1.9 0.025 - NAD+ mutant enzyme K328V, at pH 7.5 and 25°C 740079 1.17.1.9 0.0259 - NAD+ in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C 696475 1.17.1.9 0.026 - NAD+ pH 7.5, 30°C, fusion enzyme F-S3-L 742376 1.17.1.9 0.028 - NAD+ pH 6.5, 30°C 673381 1.17.1.9 0.03 - FMN - 288083 1.17.1.9 0.0303 - NAD+ recombinant enzyme, at pH 7.5 and 25°C 740079 1.17.1.9 0.032 - NAD+ - 288085 1.17.1.9 0.035 - NAD+ mutant enzyme A198G, pH and temperature not specified in the publication 741547 1.17.1.9 0.035 - NAD+ pH 7.5, 30°C, heated DE-52-fraction 656618 1.17.1.9 0.0365 - NAD+ pH 7.0, 30°C, wild-type enzyme 654505 1.17.1.9 0.037 - 2,6-dichlorophenolindophenol formate dehydrogenase I, reaction with formate 288088 1.17.1.9 0.037 - formate mutant enzyme N187S/T321S, at pH 7.2 and 30°C 740308 1.17.1.9 0.037 - NAD+ pH 7.5, 30°C, fusion enzyme F-R1-L 742376 1.17.1.9 0.037 - NADP+ mutant enzyme D221A, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.03839 - NAD+ at pH 7.5, 25°C 711549 1.17.1.9 0.039 - NAD+ pH 7.5, 30°C, fusion enzyme F-DL-L 742376 1.17.1.9 0.04 - NADP+ Km above 0.04 mM, wild type enzyme, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.041 - NAD+ wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.042 - 2,6-dichlorophenolindophenol formate dehydrogenase II, reaction with formate 288088 1.17.1.9 0.043 - NAD+ wild type enzyme, in the absence of 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C 724766 1.17.1.9 0.045 - NAD+ mutant enzyme A198G, pH and temperature not specified in the publication 741547 1.17.1.9 0.045 - NAD+ pH 7.5, 30°C, fusion enzyme F-R3-L 742376 1.17.1.9 0.048 - NAD+ pH 7.0, 30°C 655047 1.17.1.9 0.05 - NADH in 0.1 M sodium phosphate (pH 6.8), at 37°C 724039 1.17.1.9 0.051 - NAD+ pH 7.5, 30°C, fusion enzyme F-R2-L 742376 1.17.1.9 0.052 - NAD+ pH 7.5, 30°C, fusion enzyme F-S2-L 742376 1.17.1.9 0.0536 - NAD+ wild type enzyme, at 30°C and pH 7.0 739917 1.17.1.9 0.054 - NAD+ pH 7.0, 30°C, recombinant enzyme 655026 1.17.1.9 0.055 - NAD+ mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.057 - NAD+ pH 7.0, 30°C 655057 1.17.1.9 0.0571 - formate at pH 8.0 and 22°C 740581 1.17.1.9 0.06 - NAD+ at pH 7.0 and 25°C 763261 1.17.1.9 0.06 - NAD+ wild type enzyme, pH and temperature not specified in the publication 741547 1.17.1.9 0.066 - NAD+ pH 7.5, 30°C, fusion enzyme F-S1-L 742376 1.17.1.9 0.068 - NAD+ - 288104 1.17.1.9 0.07 - NAD+ - 288108 1.17.1.9 0.07 - NAD+ pH 7.0, 30°C 655461 1.17.1.9 0.07 - NAD+ wild-type enzyme and mutant enzyme Q313E 288118 1.17.1.9 0.0742 - NAD+ mutant enzyme A10C, at 30°C and pH 7.0 739917 1.17.1.9 0.075 - formate wild type enzyme, at pH 7.2 and 30°C 740308 1.17.1.9 0.075 - NAD+ pH 7.5, 30°C, enzyme from DE-52 chromatography 656618 1.17.1.9 0.075 - NAD+ pH 7.5, 30°C, enzyme from Sephadex G-15 desalted fraction 656618 1.17.1.9 0.076 - NAD+ pH 7.5, 30°C, mitochondrial enzyme 656618 1.17.1.9 0.077 - formate - 288090 1.17.1.9 0.078 - NAD+ pH 7.5, 30°C, enzyme expressed in tobacco 656618 1.17.1.9 0.08 - formate mutant enzyme N187S, at pH 7.2 and 30°C 740308 1.17.1.9 0.08 - NAD+ in 0.1 M potassium phosphate buffer pH 7.0, at 30°C 710713 1.17.1.9 0.08 - NAD+ wild type enzyme, pH and temperature not specified in the publication 741547 1.17.1.9 0.083 - NAD+ at pH 6.5 and 30°C 724094 1.17.1.9 0.084 - formate mutant enzyme T321S, at pH 7.2 and 30°C 740308 1.17.1.9 0.084 - NADP+ mutant enzyme C145S/A198G/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.09 - NAD+ - 287889, 288123 1.17.1.9 0.091 - NAD+ in 100 mM Tris-HCl (pH 7.0), at 30°C 725222 1.17.1.9 0.1 - benzyl viologen - 288094 1.17.1.9 0.1 - formate - 288089 1.17.1.9 0.1 - NAD+ - 288098, 288117 1.17.1.9 0.1 - NAD+ wild-type enzyme 288121 1.17.1.9 0.105 - NAD+ formate dehydrogenase I, reaction with formate 288088, 288092 1.17.1.9 0.11 - NAD+ - 288086, 288101 1.17.1.9 0.11 - NAD+ formate dehydrogenase II, reaction with formate 288088, 288092 1.17.1.9 0.11 - NAD+ pH 7.0, 37°C, wild-type enzyme 288102 1.17.1.9 0.118 - reduced 1-methyl-1'-aminoethyl-4,4'-bipyridinium salt at pH 7.4, temperature not specified in the publication 740320 1.17.1.9 0.1191 - NAD+ mutant enzyme A10C/I239C, at 30°C and pH 7.0 739917 1.17.1.9 0.13 - formate formate dehydrogenase II, reaction with NAD+ 288088, 288092 1.17.1.9 0.13 - NAD+ mutant enzyme D195Q/Y196P, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 0.13 - NAD+ mutant enzyme D195Q/Y196P, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 0.13 - NAD+ mutant enzyme D195S/Y196A, at pH 8.0 and 25°C 743846 1.17.1.9 0.13 - NADP+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.25 M formate 654505 1.17.1.9 0.1322 - NAD+ mutant enzyme I239C, at 30°C and pH 7.0 739917 1.17.1.9 0.135 - formate formate dehydrogenase I, reaction with NAD+ 288088, 288092 1.17.1.9 0.14 - NAD+ - 288110 1.17.1.9 0.146 - formate - 288083 1.17.1.9 0.147 - NADP+ mutant enzyme C145S/A198G/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 30°C 724079 1.17.1.9 0.16 - NAD+ in presence of formate 288093 1.17.1.9 0.16 - NADP+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate 654505 1.17.1.9 0.168 - NAD+ mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.17 - formate pH 7.0, 37°C, mutant enzyme R284Q 288102 1.17.1.9 0.17 - NAD+ mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.17 - thio-NAD+ - 288085 1.17.1.9 0.173 - NAD+ at pH 9.0 and 30°C 740431 1.17.1.9 0.177 - NAD+ wild type enzyme, in the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C 724766 1.17.1.9 0.18 - NAD+ - 288104 1.17.1.9 0.18 - NADH 25°C, pH not specified in the publication 746279 1.17.1.9 0.19 - 3-pyridinecarboxaldehyde-NAD+ - 288085 1.17.1.9 0.19 - formate wild type enzyme, at pH 9.0 and 25°C 740078 1.17.1.9 0.19 - NADP+ mutant enzyme D221S, pH and temperature not specified in the publication 741547 1.17.1.9 0.2 - 2,6-dichlorophenolindophenol formate dehydrogenase I or formate dehydrogenase II, reaction with NADH 288088, 288092 1.17.1.9 0.2 - NAD+ - 288083 1.17.1.9 0.212 - reduced methyl viologen at pH 7.4, temperature not specified in the publication 740320 1.17.1.9 0.22 - NAD+ mutant enzyme D195S/Q197T, at pH 8.0 and 25°C 743846 1.17.1.9 0.23 - NAD+ mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.24 - benzyl viologen formate dehydrogenase II, reaction with formate 288088, 288092 1.17.1.9 0.24 - NADP+ mutant enzyme D221G/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.25 - benzyl viologen formate dehydrogenase I, reaction with formate 288088, 288092 1.17.1.9 0.26 - formate reduction of NAD+ 288095 1.17.1.9 0.27 - NAD+ - 288109 1.17.1.9 0.27 - NADP+ mutant enzyme A198G/D221Q, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.277 - formate with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C 740431 1.17.1.9 0.278 - formate with ferricyanide as cosubstrate, at pH 9.0 and 30°C 740431 1.17.1.9 0.281 - formate with NAD+ as cosubstrate, at pH 9.0 and 30°C 740431 1.17.1.9 0.292 - reduced 1-methyl-1'-carboxymethyl-4,4'-bipyridinium salt at pH 7.4, temperature not specified in the publication 740320 1.17.1.9 0.3 - NAD+ mutant enzyme C255S 288121 1.17.1.9 0.31 - NADP+ mutant enzyme D221G, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.32 - HCO3- at pH 7.0 and 25°C 741359 1.17.1.9 0.371 - reduced 1,1'-dicarboxyl-4,4'-bipyridinium salt at pH 7.4, temperature not specified in the publication 740320 1.17.1.9 0.39 - NADP+ mutant enzyme D221Q, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.4 - CO2 at pH 6.0 and 35°C 743715 1.17.1.9 0.43 - CO2 25°C, pH not specified in the publication 746279 1.17.1.9 0.43 - NADP+ mutant enzyme D221S, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.44 - formate at pH 7.0 and 30°C 740775 1.17.1.9 0.45 - S-formylglutathione - 80983 1.17.1.9 0.46 - ferricyanide formate dehydrogenase I, reaction with formate 288088, 288092 1.17.1.9 0.472 - formate at pH 7.5, 25°C 711549 1.17.1.9 0.5 - ferricyanide formate dehydrogenase II, reaction with formate 288088, 288092 1.17.1.9 0.54 - deamino-NAD+ - 288085 1.17.1.9 0.54 - NAD+ mutant enzyme A198G/D221S, pH and temperature not specified in the publication 741547 1.17.1.9 0.57 - NADP+ mutant enzyme C145S/D221G/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.59 - NADP+ mutant enzyme C145S/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.6 - formate - 288113 1.17.1.9 0.6 - NAD+ mutant enzyme C255M 288121 1.17.1.9 0.62 - ferricyanide formate dehydrogenase II, reaction with NADH 288088, 288092 1.17.1.9 0.62 - formate in presence of NAD+ 288093 1.17.1.9 0.62 - NAD+ wild type enzyme, at pH 8.0 and 25°C 743846 1.17.1.9 0.623 - NAD+ pH 7.5, 30°C, wild-type enzyme 742376 1.17.1.9 0.62322 - NAD+ at pH 7.5 and 30°C 742376 1.17.1.9 0.64 - NAD+ - 288090 1.17.1.9 0.69 - ferricyanide formate dehydrogenase I, reaction with NADH 288088, 288092 1.17.1.9 0.7 - NAD+ mutant enzyme D195S/Y196L, at pH 8.0 and 25°C 743846 1.17.1.9 0.71 - NAD+ mutant enzyme D221S, pH and temperature not specified in the publication 741547 1.17.1.9 0.74 - NADP+ mutant enzyme C145S/D221A/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 0.78 - HCO3- at pH 7.0 and 25°C 741359 1.17.1.9 0.82 - formate at pH 6.5 and 30°C 724094 1.17.1.9 0.9 - formate mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 0.92 - NADP+ mutant enzyme C145S/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 30°C 724079 1.17.1.9 0.96 - NAD+ mutant enzyme D195Q, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 0.96 - NAD+ mutant enzyme D195Q, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 1.09 - NAD+ mutant enzyme C145S/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 30°C 724079 1.17.1.9 1.1 - formate mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 1.1 - NAD+ mutant enzyme D195S, at pH 8.0 and 25°C 743846 1.17.1.9 1.1 - S-formylglutathione - 288108 1.17.1.9 1.16 - 3-acetylpyridine-NAD+ - 288085 1.17.1.9 1.2 - formate mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 1.2 - NAD+ 25°C, pH not specified in the publication 746279 1.17.1.9 1.2 - NAD+ at pH 10.5 and 35°C 743715 1.17.1.9 1.3 - formate mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 1.4 - NADP+ mutant enzyme Q197V, at pH 8.0 and 25°C 743846 1.17.1.9 1.5 - formate wild type enzyme, at pH 8.0 and 37°C 739827 1.17.1.9 1.5 - formate wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 1.5 - formate wild type enzyme, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C 726427 1.17.1.9 1.5 - NAD+ mutant enzyme D195S, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 1.5 - NAD+ mutant enzyme D195S, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 1.6 - formate pH 7.0, 30°C 655047 1.17.1.9 1.67 - formate and 6.25 mM at the second catalytic site 288112 1.17.1.9 1.67 - formate pH 7.0, 30°C 655461 1.17.1.9 1.7 - formate with NAD+ as electron acceptor 288085 1.17.1.9 1.7 - NADP+ mutant enzyme D195Q/Y196H, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 1.7 - NADP+ mutant enzyme D195Q/Y196H, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 1.7 - phenyl formate in 100 mM potassium phosphate buffer at pH 7.5, at 30°C 726088 1.17.1.9 1.79 - NAD+ at pH 7.4 and 25°C 740874 1.17.1.9 1.8 - NAD+ mutant enzyme D195Q/Y196H, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 1.8 - NAD+ mutant enzyme D195Q/Y196H, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 2 - benzyl viologen formate dehydrogenase II, reaction with NADH 288088 1.17.1.9 2 - NADP+ mutant enzyme D195S/Y196L, at pH 8.0 and 25°C 743846 1.17.1.9 2 3 formate mutant enzyme D221A, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 2.02 - NAD+ mutant enzyme V120S/N187D, at pH 9.5 and 30°C 739921 1.17.1.9 2.1 - formate - 80983 1.17.1.9 2.1 - formate mutant enzyme A267M, at pH 8.0 and 37°C 739827 1.17.1.9 2.2 - formate at pH 7.5 and 25°C 742752 1.17.1.9 2.2 - formate mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C 739827 1.17.1.9 2.2 - NADP+ mutant enzyme D195S/Q197V, at pH 8.0 and 25°C 743846 1.17.1.9 2.3 - formate mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C 739827 1.17.1.9 2.3 - formate mutant enzyme Y160E, at pH 8.0 and 25°C 725746 1.17.1.9 2.3 - NAD+ mutant enzyme D195S/Y196S, at pH 8.0 and 25°C 743846 1.17.1.9 2.4 - formate mutant enzyme A267M/I272V, at pH 8.0 and 37°C 739827 1.17.1.9 2.4 - formate pH 7.0, 30°C 655057 1.17.1.9 2.5 - formate mutant enzyme V120S/N187D, at pH 9.5 and 30°C 739921 1.17.1.9 2.5 - formate pH 6.5, 30°C 673381 1.17.1.9 2.6 - glyoxylate mutant E141Q, 30°C, pH 7.0 669327 1.17.1.9 2.7 - formate mutant enzyme Y160R, at pH 8.0 and 25°C 725746 1.17.1.9 2.8 - formate mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C 739827 1.17.1.9 2.9 - formate mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 2.9 - methyl formate in 100 mM potassium phosphate buffer at pH 7.5, at 30°C 726088 1.17.1.9 3 - formate - 288101 1.17.1.9 3.1 - formate - 288094 1.17.1.9 3.1 - formate 20°C, pH 8, mutant enzyme T69V 655105 1.17.1.9 3.2 - formate wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 3.3 - formate - 288123 1.17.1.9 3.3 - formate at pH 7.0 and 25°C 741359 1.17.1.9 3.3 - NAD+ mutant enzyme Q197V, at pH 8.0 and 25°C 743846 1.17.1.9 3.3 - NADP+ mutant enzyme D195A, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 3.3 - NADP+ mutant enzyme D195A, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 3.4 - NAD+ wild type enzyme, at pH 9.5 and 30°C 739921 1.17.1.9 3.5 - NADP+ in 100 mM Tris-HCl (pH 7.0), at 30°C 725222 1.17.1.9 3.6 - formate isozyme FDH2 686800 1.17.1.9 3.6 - formate mutant enzyme H387M, at pH 9.0 and 25°C 740078 1.17.1.9 3.6 - formate wild type enzyme, at pH 9.5 and 30°C 739921 1.17.1.9 3.7 - formate mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 3.7 - NADP+ mutant enzyme D195Q/Y196P, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 3.7 - NADP+ mutant enzyme D195Q/Y196P, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 3.8 - NAD+ mutant enzyme N187D, at pH 9.5 and 30°C 739921 1.17.1.9 3.9 - formate mutant enzyme N187E, at pH 8.0 and 25°C 725746 1.17.1.9 3.9 - NAD+ mutant enzyme D195S/Q197V, at pH 8.0 and 25°C 743846 1.17.1.9 4 - formate mutant enzyme Q313E 288118 1.17.1.9 4 - formate recombinant enzyme, at pH 7.5 and 25°C 740079 1.17.1.9 4.1 - ethyl formate in 100 mM potassium phosphate buffer at pH 7.5, at 30°C 726088 1.17.1.9 4.1 - formate isozyme FDH1 686800 1.17.1.9 4.1 - formate mutant enzyme F290S, at pH 8.0 and 37°C 739827 1.17.1.9 4.1 - formate mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 4.1 - formate mutant enzyme F290S, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C 726427 1.17.1.9 4.1 - NAD+ mutant enzyme C145S/A198G/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 30°C 724079 1.17.1.9 4.2 - formate wild type enzyme, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 4.4 - formate in 100 mM potassium phosphate buffer at pH 7.5, at 30°C 726088 1.17.1.9 4.4 - formate mutant enzyme N147R, at pH 8.0 and 25°C 725746 1.17.1.9 4.4 - formate mutant enzyme Y302R, at pH 8.0 and 25°C 725746 1.17.1.9 4.5 - formate mutant enzyme F290N, at pH 8.0 and 37°C 739827 1.17.1.9 4.5 - formate mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 4.5 - formate mutant enzyme F290N, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C 726427 1.17.1.9 4.5 - NADP+ mutant enzyme D195Q, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 4.5 - NADP+ mutant enzyme D195Q, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 4.6 - glyoxylate mutant E141N, 30°C, pH 7.0 669327 1.17.1.9 4.6 - NADP+ mutant enzyme D195S/Q197T, at pH 8.0 and 25°C 743846 1.17.1.9 4.7 - formate - 288120 1.17.1.9 4.7 - NAD+ mutant enzyme D195S 288122 1.17.1.9 4.7 - NAD+ pH 8.0, 20°C, mutant enzyme D195S 288122 1.17.1.9 4.7 - propyl formate in 100 mM potassium phosphate buffer at pH 7.5, at 30°C 726088 1.17.1.9 4.75 - formate wild type enzyme, at pH 8.0 and 25°C 725746, 743846 1.17.1.9 4.8 - NAD+ mutant enzyme D195A, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 4.8 - NAD+ mutant enzyme D195A, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 5 - formate mutant enzyme F290D, at pH 8.0 and 37°C 739827 1.17.1.9 5 - formate mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 5 - formate mutant enzyme F290D, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C 726427 1.17.1.9 5 - formate mutant enzyme K328V, at 20°C, after 2 weeks or 4 months of enzyme storage 689904 1.17.1.9 5 - formate mutant enzyme K328V, at pH 7.5 and 25°C 740079 1.17.1.9 5 - NADP+ mutant enzyme D195S, at pH 8.0 and 25°C 743846 1.17.1.9 5.01 - NAD+ mutant enzyme D195N, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 5.01 - NAD+ mutant enzyme D195N, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 5.1 - formate mutant enzyme Q105R, at pH 8.0 and 25°C 725746 1.17.1.9 5.1 - NAD+ mutant enzyme D195Q/Y196S, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 5.1 - NAD+ mutant enzyme D195Q/Y196S, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 5.2 - formate at pH 7.4 and 25°C 740874 1.17.1.9 5.2 - formate mutant enzyme I239C, at 30°C and pH 7.0 739917 1.17.1.9 5.3 - formate mutant enzyme H13E, at pH 8.0 and 25°C 725746 1.17.1.9 5.5 - formate mutant enzyme N187E/Q105R, at pH 8.0 and 25°C 725746 1.17.1.9 5.5 - formate pH 7.0, 30°C, wild-type enzyme 654505 1.17.1.9 5.5 - NAD+ pH 8.0, 20°C, wild-type enzyme 288122 1.17.1.9 5.5 - NAD+ wild-type enzyme 288122 1.17.1.9 5.6 - formate 20°C, pH 8, wild-type enzyme 655105 1.17.1.9 5.6 - formate pH 7.0, 30°C, recombinant enzyme 655026 1.17.1.9 5.6 - formate wild-type, 30°C, pH 7.0 669327 1.17.1.9 5.63 - formate wild type enzyme, at 20°C, in 20 mM triethanolamine at pH 8 685733 1.17.1.9 5.8 - formate 20°C, pH 8, mutant enzyme T226V 655105 1.17.1.9 5.8 - formate mutant enzyme A10C/I239C, at 30°C and pH 7.0 739917 1.17.1.9 5.9 - formate mutant enzyme N187E/N147R, at pH 8.0 and 25°C 725746 1.17.1.9 6 - formate oxidized mutant enzyme T169C/T226C, at 20°C, in 20 mM triethanolamine at pH 8 685733 1.17.1.9 6.1 - formate in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C 696475 1.17.1.9 6.2 - NADP+ mutant enzyme D195Q/Y196S, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 6.2 - NADP+ mutant enzyme D195Q/Y196S, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 6.2 - NADP+ mutant enzyme D195S, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 6.2 - NADP+ mutant enzyme D195S, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 6.25 - formate and 1.67 mM at the second catalytic site 288112 1.17.1.9 6.5 - CO2 wild type enzyme, pH and temperature not specified in the publication 741547 1.17.1.9 6.7 - formate mutant enzyme N187D, at pH 9.5 and 30°C 739921 1.17.1.9 7 - formate wild-type enzyme 288118 1.17.1.9 7.01 - formate at pH 7.0 and 25°C 741359 1.17.1.9 7.2 - formate 25°C, pH not specified in the publication 746279 1.17.1.9 7.2 - formate at pH 10.5 and 35°C 743715 1.17.1.9 7.3 - formate wild type enzyme, at 30°C and pH 7.0 739917 1.17.1.9 7.5 - CO2 mutant enzyme A198G, pH and temperature not specified in the publication 741547 1.17.1.9 7.5 - formate wild-type enzyme and mutant enzyme C255S 288121 1.17.1.9 7.5 - glyoxylate wild-type, 30°C, pH 7.0 669327 1.17.1.9 7.6 - formate with thio-NAD+ as electron acceptor 288085 1.17.1.9 7.6 - NAD+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.25 M formate 654505 1.17.1.9 7.7 - CO2 wild type enzyme, pH and temperature not specified in the publication 741547 1.17.1.9 7.7 - formate in 0.1 M potassium phosphate buffer pH 7.0, at 30°C 710713 1.17.1.9 7.9 - formate mutant enzyme V120S, at pH 9.5 and 30°C 739921 1.17.1.9 8 - CO2 mutant enzyme A198G, pH and temperature not specified in the publication 741547 1.17.1.9 8.2 - formate mutant enzyme A10C, at 30°C and pH 7.0 739917 1.17.1.9 8.2 - NADP+ mutant enzyme D195S/Y196S, at pH 8.0 and 25°C 743846 1.17.1.9 8.4 - NAD+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate 654505 1.17.1.9 8.5 - NADP+ mutant enzyme D195S/Y196A, at pH 8.0 and 25°C 743846 1.17.1.9 8.8 - NAD+ mutant enzyme V120S, at pH 9.5 and 30°C 739921 1.17.1.9 9 - formate with NAD+ as cosubstrate, at pH 7.0 and 25°C 763261 1.17.1.9 9.1 - NAD+ mutant enzyme F290S, at pH 8.0 and 37°C 739827 1.17.1.9 9.1 - NAD+ mutant enzyme F290S, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C 726427 1.17.1.9 9.3 - formate 20°C, pH 8, mutant enzyme T69V/T226V 655105 1.17.1.9 9.9 - NAD+ mutant enzyme A267M, at pH 8.0 and 37°C 739827 1.17.1.9 10 - formate - 288104 1.17.1.9 10.8 - formate reduced mutant enzyme T169C/T226C, at 20°C, in 20 mM triethanolamine at pH 8 685733 1.17.1.9 12.8 - NAD+ mutant enzyme F290D, at pH 8.0 and 37°C 739827 1.17.1.9 12.8 - NAD+ mutant enzyme F290D, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C 726427 1.17.1.9 13 - formate - 287889, 288098, 288104 1.17.1.9 13.2 - NADP+ mutant enzyme D195N, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 13.2 - NADP+ mutant enzyme D195N, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 13.3 - NAD+ mutant enzyme A267M/I272V, at pH 8.0 and 37°C 739827 1.17.1.9 13.3 - NAD+ wild type enzyme, at pH 8.0 and 37°C 739827 1.17.1.9 13.3 - NAD+ wild type enzyme, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C 726427 1.17.1.9 14 - NAD+ mutant enzyme F290N, at pH 8.0 and 37°C 739827 1.17.1.9 14 - NAD+ mutant enzyme F290N, in 0.1 M sodium phosphate buffer, pH 7.0, at 30°C 726427 1.17.1.9 14.1 - NAD+ mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C 739827 1.17.1.9 15 - formate - 288086, 288109 1.17.1.9 15 - formate pH 7.0, 37°C, wild-type enzyme 288102 1.17.1.9 16 - formate - 288110 1.17.1.9 16 - formate mutant enzyme K47E, at 20°C, after 2 weeks of enzyme storage 689904 1.17.1.9 16.1 - NAD+ mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C 739827 1.17.1.9 19.2 - formate mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 19.6 - formate with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.0), at 30°C 725222 1.17.1.9 20 - formate mutant enzyme C255M 288121 1.17.1.9 20 - formate mutant enzyme K47E, at 20°C, after 4 months of enzyme storage 689904 1.17.1.9 20 - formate native wild type enzyme, at 20°C, after 2 weeks of enzyme storage 689904 1.17.1.9 20.3 - NAD+ mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C 739827 1.17.1.9 22 - formate - 288117 1.17.1.9 22.7 - formate mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 25 - formate mutant E141N, 30°C, pH 7.0 669327 1.17.1.9 25 - NAD+ mutant enzyme K328V, at 20°C, after 2 weeks of enzyme storage 689904 1.17.1.9 25 - NAD+ mutant enzyme K47E, at 20°C, after 2 weeks of enzyme storage 689904 1.17.1.9 27.5 - formate mutant enzyme H387K, at pH 9.0 and 25°C 740078 1.17.1.9 29.5 - NADP+ in 0.1 M potassium phosphate buffer, pH 7.5, at 30°C 696475 1.17.1.9 29.8 - formate mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication 740027 1.17.1.9 30 - formate with 3-pyridinecarboxaldehyde-NAD+ as electron acceptor 288085 1.17.1.9 30 - NAD+ mutant enzyme K328V, at 20°C, after 4 months of enzyme storage 689904 1.17.1.9 30 50 CO2 - 288092 1.17.1.9 31 - formate mutant E141Q, 30°C, pH 7.0 669327 1.17.1.9 32 - CO2 mutant enzyme D221S, pH and temperature not specified in the publication 741547 1.17.1.9 35 - formate native wild type enzyme, at 20°C, after 4 months of enzyme storage 689904 1.17.1.9 37 - NADP+ at pH 7.0 and 25°C 763261 1.17.1.9 38 - NADP+ Km above 38 mM, wild type enzyme, in 0.1 M potassium phosphate buffer (pH 7.5) 686705 1.17.1.9 38 - NADP+ Km above 38 mM, wild type enzyme, in 0.1 M potassium phosphate buffer (pH 7.5), at 37°C 686705 1.17.1.9 39.1 - formate with NADP+ as cosubstrate, in 100 mM Tris-HCl (pH 7.0), at 30°C 725222 1.17.1.9 40 - CO2 - 288089 1.17.1.9 40 - formate - 288108 1.17.1.9 40 - formate mutant enzyme D221G, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 40 - NAD+ mutant enzyme K47E, at 20°C, after 4 months of enzyme storage 689904 1.17.1.9 41 - formate mutant enzyme D221S, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 46 - formate mutant enzyme D221Q, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 50 - NAD+ native wild type enzyme, at 20°C, after 2 weeks of enzyme storage 689904 1.17.1.9 57 - formate mutant enzyme C145S/D221A/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 62 - formate mutant enzyme D221G/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 69 - formate mutant enzyme C145S/D221G/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 74.6 - NAD+ isozyme FDH1 686800 1.17.1.9 81 - formate mutant enzyme C145S/A198G/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 83 - formate mutant enzyme C145S/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 90 - NAD+ native wild type enzyme, at 20°C, after 4 months of enzyme storage 689904 1.17.1.9 98 - formate mutant enzyme C145S/A198G/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 30°C 724079 1.17.1.9 100 - formate with NADP+ as cosubstrate, at pH 7.0 and 25°C 763261 1.17.1.9 113 - formate mutant enzyme C145S/D221Q/C255V, in 0.1 M sodium phosphate buffer (pH 7.0), at 30°C 724079 1.17.1.9 127 - formate mutant enzyme A198G/D221Q, in 0.1 M sodium phosphate buffer (pH 7.0), at 22°C 724079 1.17.1.9 140 - NAD+ isozyme FDH2 686800 1.17.1.9 145 - formate pH 7.0, 37°C, mutant enzyme R284Q 288102 1.17.1.9 335 - formate with 3-acetylpyridine-NAD+ as electron acceptor 288085 1.17.1.9 362 - formate mutant enzyme R587K, at pH 9.0 and 25°C 740078 1.17.1.9 1000 - formate pH 7.0, 30°C, mutant enzyme D196A/Y197R, reaction with NADP+ 654505