1.14.15.8 additional information - additional information KM-value for the deoxicorticosterone 15beta-hydroxylase activity of recombinant BmCYP106A2 when using the NADPH/Anabaena ferredoxin electron donor system and different electron carrier proteins. Use of different AnFld mutants at positions W57, I59 and I92, which alter their aromatic and hydrophobic character, as electron donor to BmCYP106A2 696462 1.14.15.8 0.0007 - progesterone pH 7.4, 30°C, mutant K27R/I71T/I215T 697323 1.14.15.8 0.0009 - progesterone pH 7.4, 30°C, mutant I86T 697323 1.14.15.8 0.001 - progesterone pH 7.4, 30°C, mutant E90V/D185G 697323 1.14.15.8 0.0012 - deoxycorticosterone pH 7.4, 30°C, mutant I86T 697323 1.14.15.8 0.0015 - deoxycorticosterone pH 7.4, 30°C, wild-type enzyme 697323 1.14.15.8 0.0015 - progesterone pH 7.4, 30°C, wild-type enzyme 697323 1.14.15.8 0.0019 - deoxycorticosterone pH 7.4, 30°C, mutant E90V/D185G 697323 1.14.15.8 0.0024 - deoxycorticosterone pH 7.4, 30°C, mutant K27R/I71T/I215T 697323 1.14.15.8 0.0024 - progesterone pH 7.4, 30°C, mutant A395L 697323 1.14.15.8 0.00314 - reduced ferredoxin pH 7.4, 30°C, wild-type ferredoxin from the cyanobacterium Anabaena PCC 7119 696462 1.14.15.8 0.0042 - deoxycorticosterone pH 7.4, 30°C, mutant A395L 697323 1.14.15.8 0.0043 - progesterone pH 7.4, 30°C, mutant S394I 697323 1.14.15.8 0.0046 - deoxycorticosterone pH 7.4, 30°C, mutant G397P 697323 1.14.15.8 0.0047 - deoxycorticosterone pH 7.4, 30°C, mutant S394I 697323 1.14.15.8 0.0049 - progesterone pH 7.4, 30°C, mutant Q398S 697323 1.14.15.8 0.005 - progesterone pH 7.4, 30°C, mutant G397P 697323 1.14.15.8 0.0056 - deoxycorticosterone pH 7.4, 30°C, mutant Q398S 697323 1.14.15.8 0.00734 - reduced flavodoxin pH 7.4, 30°C, wild-type flavodoxin from the cyanobacterium Anabaena PCC 7119 696462 1.14.15.8 0.037 - 11-deoxycortisol pH 7.4, 30°C, mutant A106T/Q189K/T399S/R409L, mutant D153V/I214F 693956 1.14.15.8 0.059 - 11-deoxycortisol pH 7.4, 30°C, mutant A106T 693956 1.14.15.8 0.06 - deoxycorticosterone 22°C 698682 1.14.15.8 0.066 - 11-deoxycortisol pH 7.4, 30°C, mutant D217V 693956 1.14.15.8 0.068 - 11-deoxycortisol pH 7.4, 30°C, mutant A106T/R409L 693956 1.14.15.8 0.093 - 11-deoxycortisol pH 7.4, 30°C, CYP106A2 wild-type enzyme 693956 1.14.15.8 0.095 - corticosterone 22°C 698682 1.14.15.8 0.143 - progesterone 22°C 698682 1.14.15.8 0.251 - progesterone pH 7.4, 30°C, for determination of Km-value the formation of the product 15beta-hydroxy-progesterone is determined 695981 1.14.15.8 0.45 - 20alpha-dihydroprogesterone 22°C 698682 1.14.15.8 0.56 - 17alpha-hydroxyprogesterone 22°C 698682 1.14.15.8 0.625 - 4-androstene-3,17-dione 22°C 698682 1.14.15.8 1.11 - testosterone 22°C 698682