1.1.1.71 additional information - additional information Michaelis-Menten kinetics 761529 1.1.1.71 0.00079 - NADPH - 287277 1.1.1.71 0.00433 - NADPH - 287277 1.1.1.71 0.005 - NADPH with benzaldehyde as cosubstrate, at pH 7.5 and 30°C 740200 1.1.1.71 0.006 - Butanal enzyme from strain NRRL B592, at pH 7.5 and 25°C 287279 1.1.1.71 0.006 - Butanal strain B592 287279 1.1.1.71 0.0066 - trans-2-nonenal - 287278 1.1.1.71 0.008 - NADPH with cyclohexanone as cosubstrate, at pH 7.5 and 30°C 740200 1.1.1.71 0.0107 - methylglyoxal - 287278 1.1.1.71 0.011 - NADH substrates NADH and butanal, pH 5.5, 30°C 761529 1.1.1.71 0.02 - NADP+ 60°C, pH 10.5, cosubstrate: 2,5-hexandiol 726699 1.1.1.71 0.02 - NADPH 60°C, pH 7.5 726712 1.1.1.71 0.02 - NADPH 60°C, pH 7.5, cosubstrate: pyruvaldehyde 726699 1.1.1.71 0.02 - NADPH pH 7.5, 60°C 726712 1.1.1.71 0.0202 - NADP+ - 287277 1.1.1.71 0.022 - NADP+ 60°C, pH 10.5 726712 1.1.1.71 0.022 - NADP+ pH 10.5, 60°C 726712 1.1.1.71 0.024 - NADH - 287281 1.1.1.71 0.029 - NADPH pH 6.0, 50°C 722208 1.1.1.71 0.03 - NAD+ substrates NAD+ and 1-butanol, pH 9.0, 30°C 761529 1.1.1.71 0.032 - NADPH pH 6.0, temperature not specified in the publication 726769 1.1.1.71 0.038 - NADP+ - 287277 1.1.1.71 0.05 - NADPH pH 6.0, 50°C 728288 1.1.1.71 0.059 - NADP+ oxidation of ethanol 287280 1.1.1.71 0.06 - NADP+ with benzyl alcohol as cosubstrate, at pH 9.0 and 30°C 740200 1.1.1.71 0.066 - NADP+ with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 0.071 - NADPH reduction of acrylaldehyde 287280 1.1.1.71 0.072 - NADPH reduction of acetaldehyde 287280 1.1.1.71 0.075 - NADPH moderately ethanol producing strain, pH 7.0, 55°C 738540 1.1.1.71 0.08 - all-trans-retinal pH 7.4, 37°C 655563 1.1.1.71 0.088 - NADH wild-type, pH 7.0, 55°C 738540 1.1.1.71 0.089 - NAD+ with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 0.09 - pentanal substrates NADH and pentanal, pH 5.5, 30°C 761529 1.1.1.71 0.095 - NADH reduction of acetaldehyde 287280 1.1.1.71 0.101 - NADP+ pH 10.0, 50°C 722208 1.1.1.71 0.105 - NADH with acetoin as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 0.113 - NADP+ with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 0.113 - NADPH with acetoin as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 0.125 - NADP+ oxidation of allyl alcohol 287280 1.1.1.71 0.127 - NAD+ with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 0.17 - NADH moderately ethanol producing strain, pH 7.0, 55°C 738540 1.1.1.71 0.2 - NADP+ pH 10.0, 50°C 728288 1.1.1.71 0.2 - NADP+ pH 6.0, 50°C 728288 1.1.1.71 0.2 1 NADP+ pH 10.0, temperature not specified in the publication 726769 1.1.1.71 0.22 - NADH reduction of acrylaldehyde 287280 1.1.1.71 0.25 - Butanal substrates NADH and butanal, pH 5.5, 30°C 761529 1.1.1.71 0.32 - NADPH - 287281 1.1.1.71 0.32 - NADPH pH 7.4, 37°C 655563 1.1.1.71 0.4 - all-trans-retinol pH 7.4, 37°C 655563 1.1.1.71 0.5 - NAD+ oxidation of allyl alcohol 287280 1.1.1.71 0.52 - 1-butanol substrates NAD+ and 1-butanol, pH 9.0, 30°C 761529 1.1.1.71 0.52 - NADPH substrates NADPH and butanal, pH 5.5, 30°C 761529 1.1.1.71 0.65 - NADP+ pH 7.4, 37°C 655563 1.1.1.71 0.75 - Butanal - 287277 1.1.1.71 0.83 - NAD+ oxidation of ethanol 287280 1.1.1.71 1.09 - heptan-1-ol - 287277 1.1.1.71 1.22 - hexan-1-ol - 287277 1.1.1.71 1.3 - acetaldehyde cofactor NADH 287281 1.1.1.71 1.4 - acetaldehyde cosubstrate NADH, moderately ethanol producing strain, pH 7.0, 55°C 738540 1.1.1.71 1.4 - acetaldehyde cosubstrate NADPH, moderately ethanol producing strain, pH 7.0, 55°C 738540 1.1.1.71 1.5 - 2,3-Pentanedione 60°C, pH 7.5 726699 1.1.1.71 1.6 - (R)-2-hydroxypropiophenone at pH 7.5 and 30°C 740200 1.1.1.71 1.6 - acetaldehyde wild-type, pH 7.0, 55°C 738540 1.1.1.71 2.77 - propanal substrates NADH and propanal, pH 5.5, 30°C 761529 1.1.1.71 3.4 - acetaldehyde pH 6.0, 50°C 728288 1.1.1.71 3.6 - (S)-2-Hydroxypropiophenone at pH 7.5 and 30°C 740200 1.1.1.71 3.97 - Butanal - 287277 1.1.1.71 4.8 - 2-Methoxybenzaldehyde pH 6.5, 30°C 657019 1.1.1.71 5.1 - acetaldehyde pH 6.0, 50°C 722208 1.1.1.71 5.2 - Cyclohexanol at pH 9.0 and 30°C 740200 1.1.1.71 5.5 - hexanal pH 6.5, 30°C 657019 1.1.1.71 5.8 - pentan-1-ol - 287277 1.1.1.71 6.2 - octan-1-ol - 287277 1.1.1.71 6.7 - 1-butanol pH 10.0, temperature not specified in the publication 726769 1.1.1.71 6.8 - hexan-1-ol - 287277 1.1.1.71 6.9 - Salicylaldehyde pH 6.5, 30°C 657019 1.1.1.71 8.8 - acetaldehyde cofactor NADPH 287281 1.1.1.71 9.2 - acetaldehyde pH 6.0, temperature not specified in the publication 726769 1.1.1.71 9.5 - Butanal enzyme from strain NRRL B593, at pH 7.5 and 25°C 287279 1.1.1.71 9.5 - Butanal strain B593 287279 1.1.1.71 10.8 - cyclohexanone at pH 7.5 and 30°C 740200 1.1.1.71 12.3 - benzyl alcohol at pH 9.0 and 30°C 740200 1.1.1.71 13.1 - (2S,5S)-2,5-hexanediol 60°C, pH 10.5 726699 1.1.1.71 14.1 - pentan-1-ol - 287277 1.1.1.71 15.6 - benzaldehyde at pH 7.5 and 30°C 740200 1.1.1.71 17.75 - Pyruvaldehyde 60°C, pH 7.5 726699, 726712 1.1.1.71 17.75 - Pyruvaldehyde pH 7.5, 60°C 726712 1.1.1.71 21.4 - 1-propanol pH 10.0, 50°C 728288 1.1.1.71 21.4 - 1-propanol pH 6.0, 50°C 728288 1.1.1.71 24.1 - 4-methoxybenzaldehyde pH 6.5, 30°C 657019 1.1.1.71 27.1 - trans-2-hexenal pH 6.5, 30°C 657019 1.1.1.71 30.2 - acetoin with NADH as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 44 - ethanol pH 10.0, temperature not specified in the publication 726769 1.1.1.71 52.7 - butan-1-ol - 287277 1.1.1.71 54.4 - D-xylose 60°C, pH 10.5 726712 1.1.1.71 54.4 - D-xylose pH 10.5, 60°C 726712 1.1.1.71 61.3 - meso-2,3-butanediol with NAD+ as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 78.6 - butan-1-ol - 287277 1.1.1.71 107.9 - ethanol pH 10.0, 50°C 728288 1.1.1.71 107.9 - ethanol pH 6.0, 50°C 728288 1.1.1.71 150 - benzyl alcohol - 287277 1.1.1.71 168 - 2-propanol 60°C, pH 10.5 726699, 726712 1.1.1.71 168 - 2-propanol pH 10.5, 60°C 726712 1.1.1.71 244 - (RS)-1-phenylethanol with NAD+ as cosubstrate, at pH 6.0 and 70°C 735486 1.1.1.71 727 - ethanol - 287277 1.1.1.71 1060 - NAD+ pH 7.4, 37°C 655563 1.1.1.71 1200 - NADH pH 7.4, 37°C 655563 1.1.1.71 4790 - ethanol - 287277