1.1.1.282 additional information - additional information kinetic data 654912 1.1.1.282 additional information - additional information Michaelis constant with quinate as substrate is lower than that with shikimate under optimal conditions 724089 1.1.1.282 additional information - additional information Michaelis-Menten kinetics 740724, 762146 1.1.1.282 0.0004 - NAD+ mutant K71A, in the presence of shikimate, 20°C, pH 9.0 656292 1.1.1.282 0.001 - NADP+ pH 10, 20°C, cosubstrate shikimate, both forms of quinate (shikimate) dehydrogenase 657079 1.1.1.282 0.0017 - shikimate mutant S67A, 20°C, pH 9.0 656292 1.1.1.282 0.002 - NAD+ mutant K71A, in the presence of L-quinate, 20°C, pH 9.0 656292 1.1.1.282 0.0028 - shikimate mutant Q262A, 20°C, pH 9.0 656292 1.1.1.282 0.0029 - shikimate wild type enzyme, 20°C, pH 9.0 656292 1.1.1.282 0.0032 - shikimate mutant S22A, 20°C, pH 9.0 656292 1.1.1.282 0.0032 - shikimate mutant Y39F, 20°C, pH 9.0 656292 1.1.1.282 0.005 0.012 NADPH pH 10, 20°C, cosubstrate dehydroquinate, both forms of quinate (shikimate) dehydrogenase 657079 1.1.1.282 0.0054 - L-quinate mutant Y39F, 20°C, pH 9.0 656292 1.1.1.282 0.0057 - L-quinate mutant S22A, 20°C, pH 9.0 656292 1.1.1.282 0.0068 - NAD+ mutant D107A, in the presence of shikimate, 20°C, pH 9.0 656292 1.1.1.282 0.007 - NADP+ pH 10, 20°C, cosubstrate L-quinate, both forms of quinate (shikimate) dehydrogenase 657079 1.1.1.282 0.0091 - L-quinate wild type enzyme, 20°C, pH 9.0 656292 1.1.1.282 0.0107 - NAD+ mutant Q262A, in the presence of shikimate, 20°C, pH 9.0 656292 1.1.1.282 0.0121 - NAD+ mutant S67A, in the presence of L-quinate, 20°C, pH 9.0 656292 1.1.1.282 0.0122 - NAD+ wild type enzyme, in the presence of shikimate, 20°C, pH 9.0 656292 1.1.1.282 0.0125 - NAD+ mutant Y39F, in the presence of L-quinate, 20°C, pH 9.0 656292 1.1.1.282 0.0126 - NAD+ mutant S67A, in the presence of shikimate, 20°C, pH 9.0 656292 1.1.1.282 0.0136 - L-quinate mutant Q262A, 20°C, pH 9.0 656292 1.1.1.282 0.0142 - NAD+ mutant Q262A, in the presence of L-quinate, 20°C, pH 9.0 656292 1.1.1.282 0.0157 - L-quinate mutant S67A, 20°C, pH 9.0 656292 1.1.1.282 0.0158 - NAD+ mutant T106A, in the presence of shihikimat, 20°C, pH 9.0 656292 1.1.1.282 0.0158 - NAD+ mutant Y39F, in the presence of shikimate, 20°C, pH 9.0 656292 1.1.1.282 0.0168 - NAD+ mutant S22A, in the presence of L-quinate, 20°C, pH 9.0 656292 1.1.1.282 0.018 - NAD+ mutant S22A, in the presence of shikimate, 20°C, pH 9.0 656292 1.1.1.282 0.018 - NAD+ with shikimate, pH 8.6, 30°C 739956 1.1.1.282 0.0184 - NAD+ wild type enzyme, in the presence of L-quinate, 20°C, pH 9.0 656292 1.1.1.282 0.02 - shikimate pH 9, 20°C, cosubstrate NAD+ 644536 1.1.1.282 0.041 - L-quinate pH 9, 20°C, cosubstrate NAD+ 644536 1.1.1.282 0.0578 - NAD+ mutant T106A, in the presence of L-quinate, 20°C, pH 9.0 656292 1.1.1.282 0.087 - NAD+ pH 9, 20°C, cosubstrate shikimate 644536 1.1.1.282 0.1 - NADP+ pH 9, 20°C, cosubstrate shikimate 644536 1.1.1.282 0.107 - quinate with NAD+, pH and temperature not specified in the publication 762145 1.1.1.282 0.116 - NAD+ pH 9, 20°C, cosubstrate L-quinate 644536 1.1.1.282 0.12 - shikimate pH 9, 20°C, cosubstrate NADP+ 644536 1.1.1.282 0.129 - quinate with NAD+, pH and temperature not specified in the publication 762145 1.1.1.282 0.13 - NAD+ pH 7.5, 30°C, with quinate 727115 1.1.1.282 0.15 - beta-NAD+ pH 10, cosubstrate (-)-quinate 654384 1.1.1.282 0.185 - quinate with NAD+, pH and temperature not specified in the publication 762145 1.1.1.282 0.185 - quinate with NADP+, pH and temperature not specified in the publication 762145 1.1.1.282 0.187 - shikimate with NADP+, pH 7.0, 30°C 739956 1.1.1.282 0.189 - quinate with NAD+, pH and temperature not specified in the publication 762145 1.1.1.282 0.199 - shikimate mutant T106A, 20°C, pH 9.0 656292 1.1.1.282 0.2 - 3-dehydroshikimate with NADPH, pH 7.0, 30°C 739956 1.1.1.282 0.219 - NADP+ with quinate, pH and temperature not specified in the publication 762145 1.1.1.282 0.26 - shikimate with NAD+, pH 8.6, 30°C 739956 1.1.1.282 0.271 - NADP+ with shikimate, pH and temperature not specified in the publication 762145 1.1.1.282 0.28 - NAD+ pH 9.0-9.5, 30°C, with quinate 727115 1.1.1.282 0.314 - NAD+ with shikimate, pH and temperature not specified in the publication 762145 1.1.1.282 0.321 - L-quinate with NAD+, isozyme Poptr3, pH 8.5, 22°C 740724 1.1.1.282 0.334 - L-quinate with NAD+, isozyme Poptr2, pH 8.5, 22°C 740724 1.1.1.282 0.37 - L-quinate - 286356 1.1.1.282 0.392 - shikimate with NAD+, isozyme Poptr3, pH 8.5, 22°C 740724 1.1.1.282 0.42 - 3-dehydroquinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH 724089 1.1.1.282 0.43 - NAD+ pH 8.8, 25°C, with quinate 740100 1.1.1.282 0.438 - NADP+ with quinate, pH and temperature not specified in the publication 762145 1.1.1.282 0.444 - L-quinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ 724089 1.1.1.282 0.46 - NAD+ pH 10.0-10.5, 30°C, with shikimate 727115 1.1.1.282 0.48 - nicotinamide hypoxanthine dinucleotide pH 10, cosubstrate (-)-quinate 654384 1.1.1.282 0.487 - NADP+ with shikimate, pH and temperature not specified in the publication 762145 1.1.1.282 0.5 - NADP+ pH 9, 20°C, cosubstrate L-quinate 644536 1.1.1.282 0.51 - oxidized nicotinamide 1,N6-ethanoadenine dinucleotide pH 10, cosubstrate (-)-quinate 654384 1.1.1.282 0.513 - shikimate mutant K71A, 20°C, pH 9.0 656292 1.1.1.282 0.519 - NAD+ with shikimate, pH and temperature not specified in the publication 762145 1.1.1.282 0.519 - NADP+ with shikimate, pH and temperature not specified in the publication 762145 1.1.1.282 0.555 - L-quinate pH 9, 20°C, cosubstrate NADP+ 644536 1.1.1.282 0.565 - NAD+ with quinate, pH and temperature not specified in the publication 762145 1.1.1.282 0.572 - NAD+ in the presence of shikimate 694093 1.1.1.282 0.635 - NAD+ with quinate, pH and temperature not specified in the publication 762145 1.1.1.282 0.635 - NADP+ with quinate, pH and temperature not specified in the publication 762145 1.1.1.282 0.65 - 3-acetylpyridine adenine dinucleotide pH 10, cosubstrate (-)-quinate 654384 1.1.1.282 0.677 - L-quinate pH and temperature not specified in the publication, enzyme PintaQDH with NADP+ 762146 1.1.1.282 0.7 0.8 shikimate pH 10, 20°C, cosubstrate NADP+, both forms of quinate (shikimate) dehydrogenase 657079 1.1.1.282 0.71 - NAD+ pH 8.8, 25°C, with shikimate 740100 1.1.1.282 0.783 - quinate in the presence of 2 mM NAD+ 694093 1.1.1.282 0.82 - shikimate pH and temperature not specified in the publication, enzyme PintaQDH with NADP+ 762146 1.1.1.282 0.833 - shikimate with NAD+, isozyme Poptr2, pH 8.5, 22°C 740724 1.1.1.282 0.87 - NAD+ pH 7.5, 30°C, with shikimate 727115 1.1.1.282 1 - dehydroquinate pH 10, 20°C, cosubstrate NADPH, form P1 of quinate (shikimate) dehydrogenase 657079 1.1.1.282 1.083 - NAD+ in the presence of quinate 694093 1.1.1.282 1.141 - 3-dehydroquinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADPH 724089 1.1.1.282 1.18 - shikimate - 286356 1.1.1.282 1.299 - shikimate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ 724089 1.1.1.282 1.56 - L-quinate pH 7.5, 30°C, with NAD+ 727115 1.1.1.282 1.88 - L-quinate pH 8.8, 25°C, with NAD+ 740100 1.1.1.282 2.18 - shikimate pH 8.8, 25°C, with NADP+ 740100 1.1.1.282 2.38 - L-quinate pH 9.0-9.5, 30°C, with NAD+ 727115 1.1.1.282 2.47 - t-3,t-4-dihydroxycyclohexane-c-1-carboxylate pH 10, (-)-enantiomer, cosubstrate NAD+ 654384 1.1.1.282 2.541 - L-quinate with NADPH, pH 7.0, 30°C 739956 1.1.1.282 2.56 - dihydroshikimate pH 10, (-)-enantiomer, cosubstrate NAD+ 654384 1.1.1.282 2.65 - NADP+ pH 8.8, 25°C, with shikimate 740100 1.1.1.282 2.95 - L-quinate pH 10, (-)-enantiomer, cosubstrate NAD+ 654384 1.1.1.282 3.38 - shikimate pH 8.8, 25°C, with NAD+ 740100 1.1.1.282 3.4 3.6 L-quinate pH 10, 20°C, cosubstrate NADP+, both forms of quinate (shikimate) dehydrogenase 657079 1.1.1.282 4.2 - shikimate in the presence of 2 mM NAD+ 694093 1.1.1.282 5.25 - shikimate pH 10, (-)-enantiomer, cosubstrate NAD+ 654384 1.1.1.282 5.3 - dehydroquinate pH 10, 20°C, cosubstrate NADPH, form P2 of quinate (shikimate) dehydrogenase 657079 1.1.1.282 5.933 - 3-dehydroshikimate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH 724089 1.1.1.282 10.16 - shikimate pH 7.5, 30°C, with NAD+ 727115 1.1.1.282 20.52 - L-quinate mutant T106A, 20°C, pH 9.0 656292 1.1.1.282 32.68 - L-quinate mutant K71A, 20°C, pH 9.0 656292 1.1.1.282 40.07 - shikimate mutant D107A, 20°C, pH 9.0 656292 1.1.1.282 55.88 - shikimate pH 10.0-10.5, 30°C, with NAD+ 727115