1.1.1.203 additional information - additional information Michaelis-Menten kinetics 740959 1.1.1.203 additional information - additional information Michaelis-Menten kinetics and thermodynamics 740393 1.1.1.203 additional information - additional information steady-state and stopped-flow kinetics, Michaelis-Menten kinetics, and thermodynamics 740393 1.1.1.203 0.006 - NADPH with D-glucuronic acid as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 0.02 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 0.03 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.03 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 0.04 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.04 - D-galacturonate - 698607 1.1.1.203 0.053 - beta-D-galacturonate pH 8.0, 25°C, method A 740393 1.1.1.203 0.054 - D-galacturonate - 286231 1.1.1.203 0.057 - D-galacturonate - 286230 1.1.1.203 0.06 - NAD+ pH 8.0, 25°C, method B 740393 1.1.1.203 0.078 - NAD+ pH 8.0, 25°C, method B 740393 1.1.1.203 0.08 - NAD+ - 286231 1.1.1.203 0.1 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 0.1 - D-galacturonate - 698607 1.1.1.203 0.1 - NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.106 - beta-D-galacturonate pH 8.0, 25°C 740393 1.1.1.203 0.107 - beta-D-galacturonate pH 8.0, 25°C 740393 1.1.1.203 0.115 - D-galacturonic acid at pH 7.0 and 60°C 762511 1.1.1.203 0.115 - NAD+ at pH 7.0 and 60°C 762511 1.1.1.203 0.13 - beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C 762310 1.1.1.203 0.138 - beta-D-galacturonate pH 8.0, 25°C, method B 740393 1.1.1.203 0.14 - beta-D-galacturonate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C 762310 1.1.1.203 0.14 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 0.148 - beta-D-galacturonate pH 8.0, 25°C, method A 740393 1.1.1.203 0.15 - beta-D-galacturonate mutant enzyme T113V, at pH 8.0 and 25°C 762310 1.1.1.203 0.15 - D-glucuronate - 286230 1.1.1.203 0.15 - D-glucuronic acid mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C 760723 1.1.1.203 0.16 - beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C 762310 1.1.1.203 0.16 - beta-D-galacturonate pH 8.0, 25°C 740393 1.1.1.203 0.16 - D-galacturonate - 698607 1.1.1.203 0.165 - D-glucuronic acid from a beechwood xylan at pH 7.0 and 60°C 762511 1.1.1.203 0.17 - D-glucuronic acid mutant enzyme H99Y/H234K, at pH 8.0 and 30°C 760723 1.1.1.203 0.17 - NAD+ - 698607 1.1.1.203 0.18 - beta-D-galacturonate mutant enzyme A172V, at pH 8.0 and 25°C 762310 1.1.1.203 0.18 - beta-D-galacturonate mutant enzyme D246N, at pH 8.0 and 25°C 762310 1.1.1.203 0.18 - beta-D-galacturonate mutant enzyme M175I, at pH 8.0 and 25°C 762310 1.1.1.203 0.18 - beta-D-galacturonate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C 762310 1.1.1.203 0.18 - beta-D-galacturonate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C 762310 1.1.1.203 0.18 - beta-D-galacturonate wild type enzyme, at pH 8.0 and 25°C 762310 1.1.1.203 0.18 - D-glucuronic acid mutant enzyme A39P/H234K, at pH 8.0 and 30°C 760723 1.1.1.203 0.18 - NAD+ - 698607 1.1.1.203 0.19 - beta-D-galacturonate mutant enzyme Q55R, at pH 8.0 and 25°C 762310 1.1.1.203 0.19 - beta-D-galacturonate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 0.2 - beta-D-galacturonate mutant enzyme I88L, at pH 8.0 and 25°C 762310 1.1.1.203 0.2 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.2 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 0.2 - D-glucuronic acid mutant enzyme A39P/H99Y, at pH 8.0 and 30°C 760723 1.1.1.203 0.2 - D-glucuronic acid wild type enzyme, at pH 8.0 and 30°C 760723 1.1.1.203 0.2 - NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.21 - beta-D-galacturonate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 0.21 - NAD+ - 698607 1.1.1.203 0.22 - beta-D-galacturonate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 0.22 - beta-D-galacturonate mutant enzyme Y116F, at pH 8.0 and 25°C 762310 1.1.1.203 0.23 - D-glucuronic acid mutant enzyme H99Y, at pH 8.0 and 30°C 760723 1.1.1.203 0.24 - D-glucuronic acid mutant enzyme H234K, at pH 8.0 and 30°C 760723 1.1.1.203 0.24 - NAD+ pH 8.0, 25°C 740393 1.1.1.203 0.25 - D-glucuronate - 698607 1.1.1.203 0.25 - NAD+ pH 8.0, 25°C 740393 1.1.1.203 0.25 - NAD+ pH 8.0, 25°C, method A 740393 1.1.1.203 0.26 - D-glucuronic acid mutant enzyme A39P, at pH 8.0 and 30°C 760723 1.1.1.203 0.279 - beta-D-galacturonate pH 8.0, 25°C, method B 740393 1.1.1.203 0.28 - D-glucuronate - 698607 1.1.1.203 0.3 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.3 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.37 - D-glucuronate - 286231, 698607 1.1.1.203 0.37 - NAD+ pH 8.0, 25°C 740393 1.1.1.203 0.4 - NAD+ pH 8.0, 25°C, method A 740393 1.1.1.203 0.4 - NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.4 - NADH at pH 7.5 and 25°C 761270 1.1.1.203 0.41 - beta-D-galacturonate mutant enzyme I114T, at pH 8.0 and 25°C 762310 1.1.1.203 0.43 - beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C 762310 1.1.1.203 0.48 - beta-D-glucuronate pH 8.0, 25°C, method A 740393 1.1.1.203 0.5 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.65 - beta-D-glucuronate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 0.69 - beta-D-glucuronate pH 8.0, 25°C 740393 1.1.1.203 0.7 - beta-D-glucuronate wild type enzyme, at pH 8.0 and 25°C 760328 1.1.1.203 0.9 - beta-D-glucuronate mutant enzyme A41P/H101N/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 0.9 - beta-D-glucuronate mutant enzyme A41P/H101Y/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 0.9 - beta-D-glucuronate mutant enzyme H101N/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 0.9 - beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C 762310 1.1.1.203 0.95 - beta-D-glucuronate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C 762310 1.1.1.203 0.95 - D-galacturonic acid with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 1 - beta-D-glucuronate mutant enzyme A41P/H101N/H236R, at pH 8.0 and 25°C 760328 1.1.1.203 1 - beta-D-glucuronate mutant enzyme A41P/H101Y/H236R, at pH 8.0 and 25°C 760328 1.1.1.203 1 - beta-D-glucuronate mutant enzyme H101Y/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 1 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 1.03 - beta-D-glucuronate mutant enzyme M175I, at pH 8.0 and 25°C 762310 1.1.1.203 1.08 - beta-D-glucuronate pH 8.0, 25°C, method B 740393 1.1.1.203 1.1 - beta-D-glucuronate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C 762310 1.1.1.203 1.1 - beta-D-glucuronate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 1.2 - beta-D-glucuronate mutant enzyme A41P/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 1.2 - beta-D-glucuronate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C 762310 1.1.1.203 1.27 - beta-D-glucuronate pH 8.0, 25°C 740393 1.1.1.203 1.3 - beta-D-glucuronate mutant enzyme D246N, at pH 8.0 and 25°C 762310 1.1.1.203 1.3 - beta-D-glucuronate mutant enzyme H236K, at pH 8.0 and 25°C 760328 1.1.1.203 1.3 - beta-D-glucuronate pH 8.0, 25°C, method A 740393 1.1.1.203 1.34 - beta-D-glucuronate pH 8.0, 25°C 740393 1.1.1.203 1.5 - beta-D-glucuronate mutant enzyme I88L, at pH 8.0 and 25°C 762310 1.1.1.203 1.5 - beta-D-glucuronate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 1.6 - beta-D-glucuronate wild type enzyme, at pH 8.0 and 25°C 762310 1.1.1.203 1.7 - beta-D-glucuronate mutant enzyme A172V, at pH 8.0 and 25°C 762310 1.1.1.203 1.7 - beta-D-glucuronate mutant enzyme Q55R, at pH 8.0 and 25°C 762310 1.1.1.203 1.7 - beta-D-glucuronate mutant enzyme T113V, at pH 8.0 and 25°C 762310 1.1.1.203 1.94 - beta-D-glucuronate pH 8.0, 25°C, method B 740393 1.1.1.203 2 - beta-D-glucuronate mutant enzyme Y116F, at pH 8.0 and 25°C 762310 1.1.1.203 3.15 - D-galacturonic acid with NADH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 3.2 - beta-D-glucuronate mutant enzyme I114T, at pH 8.0 and 25°C 762310 1.1.1.203 3.3 - L-galactonate with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 3.34 - D-glucuronic acid with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 4.7 - L-Gulonic acid with NADP+ as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 5.3 - NAD+ with 0.67 mM D-galacturonate 286230 1.1.1.203 6.3 - D-glucuronic acid with NADH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 6.4 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 7.1 - D-glucurono-3,6-lactone with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 7.3 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 8.1 - L-galactono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 8.3 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 9 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 9.5 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 9.5 - L-gulono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 13.6 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 19.4 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 22.05 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 55 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 59 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 64 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 64 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 112 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 142 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 465 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 628 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959