2.7.7.18 additional information - additional information 4.82 Vmax/KM NMN 676525 2.7.7.18 additional information - additional information 7.16 Vmax/KM NaMN 676525 2.7.7.18 additional information - additional information Michaelis-Menten kinetics are observed for NMN and ATP, but saturation is not accomplished with NAMN, implying low affinity yet detectable activity with this substrate. Double-reciprocal plots show no cooperativity for this enzyme 737783 2.7.7.18 additional information - additional information steady-state kinetic analysis, sequential kinetic mechanism, bisubstrate enzyme kinetics, overview 738672 2.7.7.18 0.0017 - ATP - 703814 2.7.7.18 0.0045 - deamido-NAD+ 37°C, pH 7.5 642778 2.7.7.18 0.006 - ATP pH 7.5, 37°C, recombinant mutant K47A 738672 2.7.7.18 0.0068 - deamido-NAD+ 37°C, pH 7.4 642935 2.7.7.18 0.011 - ATP pH 7.5, 37°C, recombinant mutant T86A 738672 2.7.7.18 0.011 - ATP pH 7.5, 37°C, recombinant mutant W45A 738672 2.7.7.18 0.011 - nicotinic acid ribonucleotide mutant C43A, pH not specified in the publication, temperature not specified in the publication 761694 2.7.7.18 0.015 - nicotinic acid mononucleotide - 703814 2.7.7.18 0.017 - ATP pH 7.5, 37°C, recombinant mutant P44A 738672 2.7.7.18 0.0199 - ATP recombinant His6-tagged enzyme, pH 7.5, 37°C 739510 2.7.7.18 0.021 - nicotinamide mononucleotide range 0.021-0.032 mM 703814 2.7.7.18 0.022 - nicotinic acid ribonucleotide wild-type, pH not specified in the publication, temperature not specified in the publication 761694 2.7.7.18 0.023 - ATP pH 7.5, 37°C, recombinant mutant W117F 738672 2.7.7.18 0.025 - nicotinate ribonucleotide pH 7.5, recombinant enzyme 695360 2.7.7.18 0.029 - deamido-NAD+ 37°C, pH 7.5 642778 2.7.7.18 0.03 - nicotinate ribonucleotide 37°C, pH 7.5 637748 2.7.7.18 0.0304 - reduced nicotinamide nucleotide - 703814 2.7.7.18 0.033 - ATP pH 7.5, 37°C, recombinant mutant Q46A 738672 2.7.7.18 0.0358 - nicotinate beta-D-ribonucleotide recombinant His6-tagged enzyme, pH 7.5, 37°C 739510 2.7.7.18 0.041 - ATP pH 7.5, 37°C, recombinant wild-type enzyme 738672 2.7.7.18 0.042 - ATP pH 7.5, 37°C, recombinant mutant T12A 738672 2.7.7.18 0.043 - nicotinamide ribonucleotide 37°C, pH 7.4 642935 2.7.7.18 0.044 - ATP pH 7.5, recombinant enzyme 695360 2.7.7.18 0.06 - ATP 37°C, pH 7.5, + nicotinate ribonucleotide 642778 2.7.7.18 0.069 - NAD+ 37°C, pH 7.5 642778 2.7.7.18 0.07 - beta-nicotinate D-ribonucleotide mutant A86W/Y118N, pH 7.5, 30°C 760386 2.7.7.18 0.07 - NAD+ - 703814 2.7.7.18 0.073 - NAD+ - 703814 2.7.7.18 0.08 - nicotinate ribonucleotide 37°C, pH 7.5 642778 2.7.7.18 0.083 - diphosphate - 703814 2.7.7.18 0.104 - ATP - 703814 2.7.7.18 0.11 - beta-nicotinate D-ribonucleotide wild-type, pH 7.5, 30°C 760386 2.7.7.18 0.13 - nicotinate ribonucleotide 37°C, pH 7.5 642778 2.7.7.18 0.135 - ATP 37°C, pH 7.4 642935 2.7.7.18 0.17 - ATP pH 7.5, 37°C, recombinant mutant L164A 738672 2.7.7.18 0.17 - nicotinamide mononucleotide - 703814 2.7.7.18 0.19 - ATP pH 7.5, 37°C, recombinant mutant L164Q 738672 2.7.7.18 0.194 - nicotinic acid ribonucleotide mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication 761694 2.7.7.18 0.2 - nicotinamide ribonucleotide 37°C, pH 7.5 642778 2.7.7.18 0.27 - 3-acetylpyridine-NAD+ 37°C, pH 7.5 642778 2.7.7.18 0.323 - nicotinic acid ribonucleotide mutant C41A, pH not specified in the publication, temperature not specified in the publication 761694 2.7.7.18 0.33 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant Q46A 738672 2.7.7.18 0.37 - NAD+ 37°C, pH 7.5 642778 2.7.7.18 0.4 - nicotinamide ribonucleotide 37°C, pH 7.5 642778 2.7.7.18 0.43 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant L164Q 738672 2.7.7.18 0.456 - ATP mutant C41A/C43A, pH not specified in the publication, temperature not specified in the publication 761694 2.7.7.18 0.48 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant wild-type enzyme 738672 2.7.7.18 0.5 - ATP 37°C, pH 7.5 637748, 642778 2.7.7.18 0.52 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant L164A 738672 2.7.7.18 0.525 - ATP wild-type, pH not specified in the publication, temperature not specified in the publication 761694 2.7.7.18 0.53 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant T12A 738672 2.7.7.18 0.624 - ATP mutant C43A, pH not specified in the publication, temperature not specified in the publication 761694 2.7.7.18 0.65 - diphosphate 37°C, pH 7.5 642778 2.7.7.18 0.7 - nicotinamide mononucleotide - 703814 2.7.7.18 0.74 - 3-pyridinealdehyde-NAD+ 37°C, pH 7.5 642778 2.7.7.18 0.84 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant P44A 738672 2.7.7.18 0.98 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W45A 738672 2.7.7.18 1.1 - diphosphate 37°C, pH 7.5 642778 2.7.7.18 1.119 - diphosphate - 703814 2.7.7.18 1.13 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W117F 738672 2.7.7.18 1.186 - ATP mutant C41A, pH not specified in the publication, temperature not specified in the publication 761694 2.7.7.18 1.3 - nicotinate ribonucleotide 30°C, pH 7.5 1624 2.7.7.18 1.7 - beta-nicotinate D-ribonucleotide mutant Y84V/Y118D, pH 7.5, 30°C 760386 2.7.7.18 1.7 - NAD+ 37°C, pH 7.4 642935 2.7.7.18 2.3 - NMN 37°C, pH 7.4 642935 2.7.7.18 2.67 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant W117A 738672 2.7.7.18 2.7 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant K47A 738672 2.7.7.18 2.8 - nicotinate beta-D-ribonucleotide pH 7.5, 37°C, recombinant mutant T86A 738672 2.7.7.18 4 - ATP 30°C, pH 7.5 1624 2.7.7.18 4.2 - diphosphate 37°C, pH 7.4 642935