2.4.2.29 0.003 - guanine wild-type enzyme 644934 2.4.2.29 0.0033 - guanine mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00632 - guanine histidine-tagged mutant enzyme D89E, pH 8.5 644933 2.4.2.29 0.00016 - guanine34 in tRNA at pH 7.3 689250 2.4.2.29 additional information - additional information kinetic parameters of recombinant and native enzyme 728740 2.4.2.29 0.0005 - pre-queuine 0 mutant enzyme E235Q, Km less than 0.0005 mM, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.0009 - pre-queuine 0 wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.0007 - pre-queuine 1 wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.032 - pre-queuine 1 mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00011 - tRNA histidine-tagged mutant enzyme D89E, pH 7.3 644933