2.4.2.29 0.00039 - tRNA histidine-tagged wild-type enzyme, pH 8.5 644933 2.4.2.29 0.0005 - pre-queuine 0 mutant enzyme E235Q, Km less than 0.0005 mM, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00051 - tRNA histidine-tagged mutant enzyme D89E, pH 8.5 644933 2.4.2.29 0.00057 - guanine histidine-tagged wild-type enzyme, pH 8.5 644933 2.4.2.29 0.0007 - pre-queuine 1 wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00087 - virF minihelix RNA at pH 7.3 689250 2.4.2.29 0.0009 - pre-queuine 0 wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.0009 - tRNA guanine wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390 2.4.2.29 0.00098 - [tRNATyr]7-aminomethyl-7-carbaguanine mutant K52M 705164 2.4.2.29 0.001 - tRNA guanine mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 688390