5.4.99.B22 EDTA the complex Pus1/tRNAVal is sensitive to the presence of EDTA 21 5.4.99.B22 KCl the complex of Pus1 with tRNA is sensitive to high ionic strength. The optimal concentration of KCl for Pus1 binding is 100 mM. Above 400 mM KCl, no complex is detected 79