3.4.22.7	(2S,3S)-3-(N-[(S)-1-[N-(4-guanidinobutyl)carbamoyl]3-methylbutyl]carbamoyl)oxirane-2-carboxylic acid	i.e. E-64, complete immediate and irreversible inhibition at 10 mM after 30 min incubation	160161	
3.4.22.7	E-64	-	559	
3.4.22.7	iodoacetic acid	-	213	
3.4.22.7	additional information	1,10-phenantroline and pepstatine do not affect the activity of asclepain cII; activity of asclepain cII is inhibited by cysteine proteases inhibitors like E-64, but not by any other protease inhibitors such as 1,10-phenantroline, phenylmethanesulfonyl fluoride, and pepstatine	2	
3.4.22.7	NaCl	1 M, 70% loss of activity	42	
3.4.22.7	PCMB	-	78	
3.4.22.7	phenylmethylsulfonyl fluoride	incubation with 100 mM phenylmethylsulfonyl fluoride for 30 min decreases activity to 63% of the initial value, but inhibition is partially reverted when 12 mM cysteine is added to the reaction mixture	257	
3.4.22.7	Sodium tetrathionate	-	6592