1.13.11.48 Ca2+ 1 mM, weak inhibition 15 1.13.11.48 Co2+ 1 mM, weak inhibition 23 1.13.11.48 Cu2+ 1 mM, strong inhibition 19 1.13.11.48 Fe2+ 1 mM, strong inhibition 25 1.13.11.48 guanidine hydrochloride almost linear decrease of activity up to 1 M where activity vanishes, three-state unfolding of mutant enzyme; causes isothermal unfolding of mutant C69S in a three-state mechanism, thermodynamic analysis, overview 1048 1.13.11.48 Mg2+ 1 mM, weak inhibition 6 1.13.11.48 Mn2+ 1 mM, weak inhibition 11 1.13.11.48 Ni2+ 1 mM, strong inhibition 38 1.13.11.48 Urea causes isothermal unfolding of mutant C69S in a three-state mechanism, thermodynamic analysis, overview; nonlinear decrease of activity, activity lost at about 5 M, three-state unfolding of mutant enzyme 116 1.13.11.48 Zn2+ 1 mM, strong inhibition 14