3.5.99.7 1-amino-2,2-difluorocyclopropane-1-carboxylic acid rac-DFACC, chemical synthesis, overview. Mechanism of decomposition and inhibition of 1-aminocyclopropane-1-carboxylic acid deaminase, and Michaelis-Menten kinetics. DFACC is unstable under near-physiological conditions where it primarily decomposes via specific-base catalysis to 3-fluoro-2-oxobut-3-enoic acid. Different decomposotion mechanisms are described. Upon incubation with ACC deaminase, DFACC is a slow-dissociating inhibitor of ACC deaminase with submicromolar affinity 247567 3.5.99.7 1-amino-2-methylenecyclopopropane-1-carboxylic acid irreversible 114736 3.5.99.7 1-aminocyclopropanephosphonate - 90470 3.5.99.7 5,5'-dithiobis(2-nitrobenzoic acid) - 221 3.5.99.7 aminooxyacetic acid 0.005-1 mM, enzymatic activity is progressively inhibited as the aminooxyacetic acid concentration is increased 1554 3.5.99.7 beta-chloro-Ala - 16065 3.5.99.7 beta-chloro-Ala mechanism-based inhibition 16065 3.5.99.7 beta-fluoro-D-Ala - 96220 3.5.99.7 cyclohexylhydrazine - 96308 3.5.99.7 D-Ala - 291