3.1.1.14 additional information poor inhibition by methyl methanethiosulfonate and iodoacetamide 2 3.1.1.14 additional information the expression level and the activity of Chlase decrease significantly with the progress of the leaf-yellowing 2 3.1.1.14 additional information inhibitory effects on recombinant enzymes purified by microtiter plate method and by affinity chromatography, respectively, overview, no inhibition by iodoacetic acid, iodoacetamide, 2-mercaptoethanol, and DTT 2 3.1.1.14 Mg2+ 12% inhibition at 0.01 mM, 24 h preincubation 6 3.1.1.14 Mg2+ - 6 3.1.1.14 Mg2+ eliminates the activating effect of lecithin 6 3.1.1.14 Mn2+ 67% inhibition at 0.01 mM, 24 h preincubation 11 3.1.1.14 Mn2+ 72% residual activity at 10 mM 11 3.1.1.14 Mn2+ 78% residual activity at 10 mM 11 3.1.1.14 Mn2+ 73% residual activity at 10 mM 11