1.13.11.60	additional information	not inhibited by 1 mM KCN. CO does not inhibit the enzyme	2	
1.13.11.60	additional information	catalysis declines due to suicide inactivation; catalysis declines due to suicide inactivation. Following column chromatography, e.g. ion exchange or zinc-affinity chromatography, hydroperoxide isomerase activity is often decreased and sometimes even undetectable by TLC, whereas 8-dioxygenase activity is always present. However, storage of the enzyme for a few days on ice often restores the hydroperoxide isomerase and appeares to increase the 7,8-linoleate diol synthase activity as well	2	
1.13.11.60	additional information	GSH (1 mM), GSSG (1 mM), EDTA (1 or 10 mM), EGTA (1 or 10 mM) and H202 (10 mM) are without effect on the 8R-dioxygenase	2	
1.13.11.60	additional information	sorbitol (0.75 M, 14%), glycerol (15%) and ethylene glycol (25%) do not inhibit the enzyme	2	
1.13.11.60	additional information	suicide inactivation, activity falls to zero within a few min although substrates were available in sufficient amounts	2	
1.13.11.60	dithiothreitol	-	45	
1.13.11.60	Triton X-100	0.5%, inactivation	61	
1.13.11.60	1,10-phenanthroline	2.5 mM, 50% inhibition	62	
1.13.11.60	2-mercaptoethanol	-	63	
1.13.11.60	glycine	2%	72