5.4.3.8 4,5-Dioxovalerate competitive inhibition of pyridoxamine-phosphate form of the enzyme and mixed-type inhibition of the pyridoxal-phosphate form of the enzyme 12361 5.4.3.8 4-Amino-5-fluoro-pentanoic acid - 91631 5.4.3.8 4-Amino-5-hexenoic acid - 91632 5.4.3.8 4-Amino-5-hexynoic acid - 43409 5.4.3.8 Aminooxyacetate 20 mM, 57% inhibition 1374 5.4.3.8 Aminooxyacetate - 1374 5.4.3.8 cycloserine 20 mM, 43% inhibition 2837 5.4.3.8 D-penicillamine 20 mM, 9% inhibition 2733 5.4.3.8 gabaculine 0.005 mM, 83% inhibition. Irreversible inhibition which requires the presence of either glutamate-1-semialdehyde or 5-amino-4-oxopentanoate 1869 5.4.3.8 gabaculine complete inactivation by gabaculine when the enzyme is exposed to 5-amino-4-oxopentanoate, levulinate or 4,5-dioxovalerate in absence of glutamate 1-semialdehyde 1869 5.4.3.8 gabaculine 0.0005 mM, 50% inhibition. The inhibition is reversible up to 1 h after its addition, if the gabaculine is removed by gel filtration, before the enzyme is incubated by gel filtration, before the enzyme is incubated with substrate. Irreversible inhibition is obtained by preincubation of the enzyme at 30°C either for several hours with gabaculine alone or for a few min with both gabaculine and glutamate-1-semialdehyde 1869 5.4.3.8 gabaculine - 1869 5.4.3.8 gabaculine wild-type enzyme is inhibited, gabaculine-resistant mutant enzyme is not inhibited 1869 5.4.3.8 gabaculine potent inhibitor 1869 5.4.3.8 glyoxylate 20 mM, 27% inhibition 101 5.4.3.8 additional information no inhibition by 3-hydroxyglutamate 2 5.4.3.8 additional information 5 mM EDTA has no effect on activity 2 5.4.3.8 p-chloromercuribenzoate 0.1 mM, 45% inhibition 43 5.4.3.8 pyridoxal 5'-phosphate 5 mM, 47% inhibition 32 5.4.3.8 pyridoxal 5'-phosphate - 32