3.4.22.14 cystatin C recombinant inhibitor expressed in E. coli 1994 3.4.22.14 E-64 - 559 3.4.22.14 Fe2+ addition leads to inhibition of free actinidin whereas immobilized actinidin shows a much weaker inhibition 25 3.4.22.14 Hg2+ - 33 3.4.22.14 human kininogen domain 2 hybrids of chicken cystatin with human kininogen domain 2 sequences exhibit improved inhibition 100713 3.4.22.14 iodoacetate - 93 3.4.22.14 KCl minimal activity at 0.5-0.8 M, depending on substrate concentration. Minimal activity is 50-70% of the acitivity in absence of salt, with increase in KM-value and decrease in kcat-value. Slight reactivation above 0.8 M 79 3.4.22.14 L-kininogen - 11007 3.4.22.14 leupeptin - 217 3.4.22.14 LiCl minimal activity at 1.0-1.5 M, depending on substrate concentration. Minimal activity is 50-70% of the acitivity in absence of salt, with increase in KM-value and decrease in kcat-value. Slight reactivation above 1.5 M 815