3.2.1.62 2',4'-dinitrophenyl-2-deoxy-2-fluoro-beta-D-galactopyranoside inactivation of lactase hydrolase activity 123109 3.2.1.62 2',4'-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside inactivation of phlorizin hydrolase activity 123108 3.2.1.62 2',4'-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside rapidly inhibits phlorizin hydrolase activity by covalently binding to the glutamic acid residue of the active site, lactase activity more slowly inhibited, pre-incubation with glucal protects the lactase active site from inactivation 104384 3.2.1.62 2-fluoro-dinitrophenylglucoside specifically inhibits phlorizin hydrolase, lactase activity is inhibited slowly 55957 3.2.1.62 2-oxoglutaric acid 50% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM 6433 3.2.1.62 Ca2+ about 78% residual activity at 1 mM 15 3.2.1.62 cellobiose inhibition of galactosylceramide hydrolysis 82 3.2.1.62 cellobiose - 82 3.2.1.62 cerebroside from brain, competitive inhibition of lactase activity, Ki: 1.11 mM 8061 3.2.1.62 Co2+ about 18% residual activity at 1 mM 23 3.2.1.62 conduritol-B-epoxide 90% inactivation of lactase hydrolysis, 55% inactivation of phlorizin hydrolysis, inactivation by covalent binding to Glu1747 and Glu 1271 8072 3.2.1.62 Cu2+ about 10% residual activity at 1 mM 19 3.2.1.62 D-galactal inactivation of lactase hydrolase activity 8082 3.2.1.62 D-galactono-1,4-lactone competitive inhibition of lactase activity, Ki: 0.35 mM 2730 3.2.1.62 D-galactono-1,4-lactone competitive inhibition of lactase activity, not phlorizin activity, Ki: 2 mM 2730 3.2.1.62 D-glucal inactivation of lactase hydrolase activity 8497 3.2.1.62 D-glucono-1,5-lactone competitive inhibition of lactase activity, Ki: 0.027 mM 467 3.2.1.62 D-glucono-1,5-lactone competitive inhibition of phlorizin hydrolysis, Ki: 0.18 mM, competitive inhibition of lactase activity, Ki: 0.275 mM 467 3.2.1.62 D-glucose - 35 3.2.1.62 deoxygalactonojirimycin competitive inhibition, Ki: 0.076 mM 21583 3.2.1.62 deoxynojirimycin competitive inhibition, Ki: 0.142 mM 1922 3.2.1.62 DMSO the enzyme is inhibited by 30-50% (v/v) DMSO 896 3.2.1.62 epigallocatechin-3-gallate inhibits in vitro hydrolysis of lactose by lactase phlorizin hydrolase 2607 3.2.1.62 Fe2+ about 40% residual activity at 1 mM 25 3.2.1.62 Fe3+ about 50% residual activity at 1 mM 70 3.2.1.62 galactosylceramide competitive inhibition of glucosyldihydroceramide hydrolysis 3203 3.2.1.62 glucosyldihydroceramide competitive inhibition of galactosylceramide hydrolysis 32234 3.2.1.62 Hg+ complete inhibition at 1 mM 1756 3.2.1.62 lactose competitive to pyridoxine-5'-beta-D-glucoside 114 3.2.1.62 Maleic acid 55% inhibition for the tartaric acid-stimulated phlorizin hydrolase at 20 mM 4029 3.2.1.62 Mg2+ about 90% residual activity at 1 mM 6 3.2.1.62 Mn2+ about 50% residual activity at 1 mM 11 3.2.1.62 additional information not inhibitory: pyridoxine, 2’,4’-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside 2 3.2.1.62 additional information conduritol B epoxide only slightly inhibitory 2 3.2.1.62 additional information LPH expression appears to fall off in the villus tip of germfree pigs. LPH activity to gene expression ratio is lower in pigs conventionalized with faecal bacteria or monoassociatied with Escherichia coli compared with germfree pigs. LPH activity to gene expression ratio in pigs monoassociatied with Lactobacillus fermentum is similar to germfree pigs whereas in pigs monoassociatied with Lactobacillus fermentum and contaminated with Klebsiella pneumoniae the ratio is similar to pigs monoassociatied with Escherichia coli 2 3.2.1.62 additional information coexpression with Rab4S22N or Rab11S25N decreases the number of vesicular structures positive for LPH. Trafficking delay of LPH following Rab protein depletion in MDCK cells 2 3.2.1.62 N-(n-butyl)-deoxygalactojirimycin - 55956 3.2.1.62 n-butyldeoxygalactonojirimycin - 55983 3.2.1.62 Na+ about 93% residual activity at 1 mM 59 3.2.1.62 NaCl 30% inhibition of the tartaric acid-stimulated phlorizin hydrolase at 20 mM 42 3.2.1.62 Ni2+ about 5% residual activity at 1 mM 38 3.2.1.62 nonyl-deoxynojirimycin - 232831 3.2.1.62 NSP4 secreted non-structural rotavirus protein 66273 3.2.1.62 oxalacetic acid 50% inhibition of the tartaric acid-stimualted phlorizin hydrolase at 20 mM 32366 3.2.1.62 oxalic acid 47% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM 2093 3.2.1.62 Pb2+ about 10% residual activity at 1 mM 139 3.2.1.62 phlorizin competitive inhibition of lactase activity; Ki: 0.44 mM 1563 3.2.1.62 phlorizin competitive inhibition of lactase activity 1563 3.2.1.62 phlorizin competitive inhibition of lactase activity, Ki: 0.37 mM 1563 3.2.1.62 phlorizin competitive inhibition of lactase activity, Ki: 0.18 mM 1563 3.2.1.62 phlorizin competitive inhibition of galactosylceramide hydrolysis, Ki: 0.35 mM 1563 3.2.1.62 phlorizin competitive inhibition of lactase activity, Ki: 0.047 mM 1563 3.2.1.62 pyridoxine-5'-beta-D-glucoside substrate inhibition above 3 mM 11510 3.2.1.62 Pyruvic acid strongly inhibitory for the tartaric acid-stimulated phlorizin hydrolase 1725 3.2.1.62 SO42- strong inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM 245 3.2.1.62 sodium cholate competitive inhibition of lactase activity, in the presence of bovine serum albumin 1209 3.2.1.62 Tris(hydroxymethyl)aminomethane competitive inhibition of lactase acitiviy, phlorizin activity not affected, Ki: 12 mM 4057 3.2.1.62 Tris(hydroxymethyl)aminomethane - 4057 3.2.1.62 Tris(hydroxymethyl)aminomethane competitive inhibition of lactase activity, Ki: 5.1 mM 4057 3.2.1.62 Tris(hydroxymethyl)aminomethane competitive inhibition of lactase activity, Ki: 4.5 mM 4057 3.2.1.62 Triton X-100 strongly inhibits the activity even at a low concentration 61 3.2.1.62 Zn2+ about 8% residual activity at 1 mM 14