3.2.1.33 1,4-diaminobutane putrescine, glycogen debranching enzyme, slight inhibition 1296 3.2.1.33 1-deoxynojirimycin - 557 3.2.1.33 1-deoxynojirimycin in primary rat hepatocytes, the inhibition of glycogen breakdown reaches plateau at 100 microM with 25% inhibition and then remains unchanged 557 3.2.1.33 1-S-dimethylarsino-1-thio-beta-D-glucanopyranoside DATG, active site-directed irreversible inhibitor, inhibition: mechanism. Inactivation occurs with significant conformational change 31730 3.2.1.33 1-S-dimethylarsino-1-thio-beta-D-glucanopyranoside DATG, active site-directed irreversible inhibitor, inhibition: mechanism. Inactivation occurs with significant conformational change; glycogen phosphorylase limit dextrin and Bis-Tris protect from inactivation 31730 3.2.1.33 2,2-bis(hydroxymethyl)-2,2',2''-nitrilotriethanol Bis-Tris, reversible inhibitor 13024 3.2.1.33 2,2-bis(hydroxymethyl)-2,2',2''-nitrilotriethanol 10 mM: 97% inhibition; Bis-Tris, reversible inhibitor 13024 3.2.1.33 2,2-bis(hydroxymethyl)-2,2',2''-nitrilotriethanol Bis-Tris, reversible inhibitor; noncompetitive with glycogen phosphorylase limit dextrin, competitive with glucose 13024 3.2.1.33 2,2-bis(hydroxymethyl)-2,2',2''-nitrilotriethanol - 13024 3.2.1.33 2-(2-hydroxyethylamino)-2-hydroxymethyl-1,3-propanediol hydroxyethyltris 101804 3.2.1.33 2-(N-morpholino)ethanesulfonic acid - 102113 3.2.1.33 2-amino-1,3-propandiol - 102123 3.2.1.33 2-amino-2-methyl-1,3-propanediol - 9006 3.2.1.33 2-deoxy-2-fluoro-alpha-glucosyl fluoride very poor inhibitor, 56 mM: 17% inhibition 102136 3.2.1.33 2-hydroxyethyl-3-hydroxypropylamine HEPA, glycogen debranching enzyme 101802 3.2.1.33 3-aminopropyl-2-hydroxyethylamine DAPH, glycogen debranching enzyme 102182 3.2.1.33 5,5'-dithiobis(2-nitrobenzoic acid) 50% inhibition, reversed by glycogen 221 3.2.1.33 5,5'-dithiobis(2-nitrobenzoic acid) DTNB 221 3.2.1.33 5-amino-D-glucose nojirimycin, potent inhibitor, noncompetitive 47770 3.2.1.33 5-thio-D-glucose slight inhibition 9465 3.2.1.33 7-O-beta-D-glucopyranosyl-homonojirimycin - 74921 3.2.1.33 alpha-homonojirimycin - 39000 3.2.1.33 Amine protonated, hydroxylalkyl-substituted, noncompetitive, mechanism of inhibition 8044 3.2.1.33 Amine noncompetitive with glycogen phosphorylase limit dextrin, competitive with glucose; polyhydroxyamines, mechanism of inhibition; protonated, hydroxylalkyl-substituted, noncompetitive, mechanism of inhibition 8044 3.2.1.33 Amine - 8044 3.2.1.33 aminophenyl arsenoxide - 102458 3.2.1.33 AMP 10 mM, 86% of initial activity 30 3.2.1.33 ATP 10 mM, no activity 4 3.2.1.33 cationic buffer - 102600 3.2.1.33 Cd2+ 1 mM, no activity 52 3.2.1.33 choline very poor inhibitor 149 3.2.1.33 Co2+ 1 mM, 15% of initial activity 23 3.2.1.33 Cu2+ 1 mM, 6% of initial activity 19 3.2.1.33 cyclohexaamylose alpha-Schardinger dextrin, debranching enzyme, competitive inhibitor 4515 3.2.1.33 D-glucono-1,5-lactone glycogen debranching enzyme, noncompetitive with glycogen phosphorylase limit dextrin, competitive with glucose 467 3.2.1.33 D-glucose competitive 35 3.2.1.33 D-glucose 10 mM, 89% of initial activity 35 3.2.1.33 D-glucose 10% inhibition by 10 mM, 50% inhibition by 50 mM 35 3.2.1.33 D-glucose-1-phosphate 10 mM, 82% of initial activity 1425 3.2.1.33 D-Glucose-6-phosphate 10 mM, 89% of initial activity 2838 3.2.1.33 D-isofagomine - 74922 3.2.1.33 deoxynojirimycin reversible, specific inhibitor 1922 3.2.1.33 diethanolamine - 4809 3.2.1.33 DMSO 80.8% residual activity at 10% (v/v) 896 3.2.1.33 ethanol 83.3% residual activity at 10% (v/v) 69 3.2.1.33 ethanolamine - 456 3.2.1.33 ethylamine - 701 3.2.1.33 ethylamine slight inhibition 701 3.2.1.33 fagomine - 74920 3.2.1.33 Fe2+ 1 mM, 7% of initial activity 25 3.2.1.33 glucooligosaccharides containing one, two, or three glucose, competitive inhibitors 21306 3.2.1.33 glycogen competitive 506 3.2.1.33 glycylglycine slight inhibition 1301 3.2.1.33 glycylglycine inhibits at acidic pH 1301 3.2.1.33 glycylglycine no inhibition at 0.01 or 0.05 M 1301 3.2.1.33 guanidine muscle and liver enzyme: inhibition at very low concentrations 2023 3.2.1.33 guanidine at 0.05 M: 20% inhibition, at 0.15 M: 50% inhibition 2023 3.2.1.33 Hg2+ 1 mM, 3% of initial activity 33 3.2.1.33 hydroxylamine - 85 3.2.1.33 imidazole buffer, 50% inhibition 470 3.2.1.33 imidazole - 470 3.2.1.33 m-erythritol noncompetitive 102972 3.2.1.33 maltose competitive 86 3.2.1.33 maltotriose competitive 188 3.2.1.33 methanediimine carbodiimide in the presence of an amine inhibits glycogen debranching enzyme, transferase activity is inhibited, amylo-1,6-glucosidase , hydrolysis of alpha-glucosyl fluoride, is unaffected by carbodiimide. Slow inactivation of glucosidase activity as measured by [14C]glucose incorporation into glycogen 47845 3.2.1.33 methanol 91.6% residual activity at 10% (v/v) 83 3.2.1.33 miglitol - 3392 3.2.1.33 mono-(2-ethylhexyl)phthalate inhibits the activity of oligo-1,4-1,4 glucanotransferase in bifunctional amylo-1,6-glucosidase oligo-1,4-1,4 glucanotransferase, but not that of amylo-1,6-glucosidase 151337 3.2.1.33 additional information unaffected by hormone administration 2 3.2.1.33 additional information inhibition mechanism 2 3.2.1.33 additional information - 2 3.2.1.33 additional information no inhibition by usual sugar phosphates or nucleotide effectors of glycolysis such as AMP, ADP, ATP, uridine diphosphoglucose, glucose-1-phosphate, glucose-6-phosphate and phosphate 2 3.2.1.33 additional information inhibition mechanism; no inhibition by dithioerythritol, dithiothreitol, cadaverine, spermidine, spermine, D,L-threitol, 1-thio-beta-D-glucose 2 3.2.1.33 N,N'-bis(tris[hydroxymethyl]methyl)-1,3-diaminopropane bis(tris)propane, noncompetitive 101803 3.2.1.33 N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid slight inhibition 94175 3.2.1.33 N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid slight inhibition 103223 3.2.1.33 NH4+ slight inhibition 54 3.2.1.33 Ni2+ 1 mM, 25% of initial activity 38 3.2.1.33 organic arsenites - 103380 3.2.1.33 p-hydroxymercuribenzoate 0.3 mM: completely 98 3.2.1.33 p-hydroxymercuribenzoate - 98 3.2.1.33 phosphate buffer, inhibits at neutral pH 16 3.2.1.33 SDS irreversible inhibition by less than 0.1% SDS 124 3.2.1.33 taurine slight inhibition 595 3.2.1.33 triethylamine very poor inhibitor 10892 3.2.1.33 Tris buffer, strong inhibition 317 3.2.1.33 Tris - 317 3.2.1.33 Tris 0.02 M Tris: 50-60% inhibition 317 3.2.1.33 Tris 15 mM: 75% inhibition 317 3.2.1.33 Tris noncompetitive; Tris analogues 317 3.2.1.33 Urea muscle enzyme: reversible 95% inhibition by 2 M urea, with higher concentration, 3 M urea: time-dependent irreversible denaturation, liver enzyme: reversible 57% inhibition by 2 M urea 116 3.2.1.33 Urea muscle enzyme: reversible 95% inhibition by 2 M urea, with higher concentration, 3 M urea: time-dependent irreversible denaturation, liver enzyme: reversible 57% inhibition by 2 M urea; reversible inhibitor below 2 M, 90% inhibition by 2.6 M urea 116 3.2.1.33 Urea glucosidase-transferase: 31% inhibition at 0.21 M, 72% at 1.6 M, 98% at 3.2 M 116 3.2.1.33 Urea - 116 3.2.1.33 Zn2+ 1 mM, 4% of initial activity 14