3.1.3.32	2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione	A12B4C3	5856	
3.1.3.32	2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione	i.e. A12B4C3, a potent, noncompetitive inhibitor of the phosphatase activity of human polynucleotide kinase/phosphatase in vitro. The inhibitor can form a ternary complex with PNKP and a DNA substrate, the inhibitor does not prevent DNA from binding to the phosphatase DNA binding site, but disrupts the secondary structure of PNKP, interaction between Trp402 of PNKP and the inhibitor. A12B4C3 sensitizes A549 human lung cancer cells to the topoisomerase I poison, camptothecin, but not the topoisomerase II poison, etoposide, in a manner similar to small interferingRNAagainst PNKP	5856	
3.1.3.32	2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione	A6B4C3	39792	
3.1.3.32	2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione	A1B4C3	77449	
3.1.3.32	2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione	A39B1C2	77450	
3.1.3.32	diphosphate	no effect on 3'-phosphatase, inhibition of 5'-hydroxyl kinase	17	
3.1.3.32	EDTA	-	21	
3.1.3.32	heparin	-	227	
3.1.3.32	heparin	heparin exhibits an IC50 of 0.16 mg/ml	227	
3.1.3.32	additional information	optimization of a rapid high-throughput screening method for detection of DNA 3'-phosphatases and their inhibitors, overview	2	
3.1.3.32	additional information	sensitivity to methyl methanesulfonate of all single and double mutant combinations of nth1, apn2, tdp1 and pnk1	2	
3.1.3.32	NaCl	can replace Mg2+ at 50 mM, inhibitory at 250 mM	42	
3.1.3.32	NaCl	best stimulation at 100 mM, inhibitory above 200 mM	42	
3.1.3.32	PO43-	5'-hydroxyl kinase, little effect on 3'-phosphatase	867	
3.1.3.32	SO42-	5'-hydroxyl kinase, little effect on 3'-phosphatase	245	
3.1.3.32	tert-butyl 2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridin-1-ylcarbamate	A26B11C2	155609