3.1.1.14 additional information poor inhibition by methyl methanethiosulfonate and iodoacetamide 2 3.1.1.14 additional information the expression level and the activity of Chlase decrease significantly with the progress of the leaf-yellowing 2 3.1.1.14 additional information inhibitory effects on recombinant enzymes purified by microtiter plate method and by affinity chromatography, respectively, overview, no inhibition by iodoacetic acid, iodoacetamide, 2-mercaptoethanol, and DTT 2 3.1.1.14 Mg2+ 12% inhibition at 0.01 mM, 24 h preincubation 6 3.1.1.14 Mg2+ - 6 3.1.1.14 Mg2+ eliminates the activating effect of lecithin 6 3.1.1.14 Mn2+ 67% inhibition at 0.01 mM, 24 h preincubation 11 3.1.1.14 Mn2+ 72% residual activity at 10 mM 11 3.1.1.14 Mn2+ 78% residual activity at 10 mM 11 3.1.1.14 Mn2+ 73% residual activity at 10 mM 11 3.1.1.14 Zn2+ 60% inhibition at 0.01 mM, 24 h preincubation 14 3.1.1.14 Zn2+ - 14 3.1.1.14 Zn2+ 51% residual activity at 10 mM 14 3.1.1.14 Zn2+ 59% residual activity at 10 mM 14 3.1.1.14 Zn2+ 54% residual activity at 10 mM 14 3.1.1.14 Cu2+ 55% inhibition at 0.01 mM, 24 h preincubation 19 3.1.1.14 Cu2+ - 19 3.1.1.14 Cu2+ 39% residual activity at 10 mM 19 3.1.1.14 Cu2+ 42% residual activity at 10 mM 19 3.1.1.14 Cu2+ 34% residual activity at 10 mM 19 3.1.1.14 Co2+ 56% inhibition at 0.01 mM, 24 h preincubation 23 3.1.1.14 Co2+ 75% residual activity at 10 mM 23 3.1.1.14 Co2+ 78% residual activity at 10 mM 23 3.1.1.14 Co2+ 72% residual activity at 10 mM 23 3.1.1.14 Fe2+ 89% inhibition at 0.01 mM, 24 h preincubation 25 3.1.1.14 Fe2+ - 25 3.1.1.14 Fe2+ 31% residual activity at 10 mM 25 3.1.1.14 Fe2+ 29% residual activity at 10 mM 25 3.1.1.14 Fe2+ 35% residual activity at 10 mM 25 3.1.1.14 Hg2+ 85% inhibition at 0.01 mM, 24 h preincubation 33 3.1.1.14 Hg2+ - 33 3.1.1.14 Hg2+ 22% residual activity at 10 mM 33 3.1.1.14 Hg2+ 25% residual activity at 10 mM 33 3.1.1.14 Hg2+ 20% residual activity at 10 mM 33 3.1.1.14 N-ethylmaleimide 13% inhibition at 1 mM, 24 h preincubation 49 3.1.1.14 N-ethylmaleimide irreversible, 15.9% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C 49 3.1.1.14 N-ethylmaleimide - 49 3.1.1.14 Triton X-100 strong inhibition at 0.2-1.0% 61 3.1.1.14 Triton X-100 - 61 3.1.1.14 iodoacetamide irreversible, 6% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C 67 3.1.1.14 iodoacetamide - 67 3.1.1.14 Fe3+ 13% inhibition at 0.01 mM, 24 h preincubation 70 3.1.1.14 Fe3+ - 70 3.1.1.14 Fe3+ 73% residual activity at 10 mM 70 3.1.1.14 Fe3+ 71% residual activity at 10 mM 70 3.1.1.14 Fe3+ 78% residual activity at 10 mM 70 3.1.1.14 Ag+ 82% residual activity at 10 mM 75 3.1.1.14 Ag+ 89% residual activity at 10 mM 75 3.1.1.14 Ag+ 85% residual activity at 10 mM 75 3.1.1.14 PCMB irreversible, 92.6% inhibition at 1 mM, 1 h preincubation at pH 7.0 and 30°C 78 3.1.1.14 PCMB - 78 3.1.1.14 NEM - 89 3.1.1.14 ferricyanide 2 mM, 17% inhibition 132 3.1.1.14 diisopropyl fluorophosphate irreversible, 91% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C 244 3.1.1.14 diisopropyl fluorophosphate - 244 3.1.1.14 diisopropyl fluorophosphate noncompeptitive 244 3.1.1.14 PMSF irreversible, 89.2% inhibition at 10 mM, 1 h preincubation at pH 7.0 and 30°C 248 3.1.1.14 phenylmethylsulfonyl fluoride 69% residual activity at 10 mM 257 3.1.1.14 phenylmethylsulfonyl fluoride 58% residual activity at 10 mM 257 3.1.1.14 phenylmethylsulfonyl fluoride 65% residual activity at 10 mM 257 3.1.1.14 Al3+ 52% residual activity at 10 mM 264 3.1.1.14 Al3+ 56% residual activity at 10 mM 264 3.1.1.14 Al3+ 49% residual activity at 10 mM 264 3.1.1.14 diethyldicarbonate 83% residual activity at 10 mM 310 3.1.1.14 diethyldicarbonate 70% residual activity at 10 mM 310 3.1.1.14 diethyldicarbonate 72% residual activity at 10 mM 310 3.1.1.14 acetone activates at lower concentration, Chlase2 reaches its maximal activity in 40% acetone, in 40-60% acetone the activity is reduced, overview 333 3.1.1.14 phenylmethanesulfonyl fluoride - 827 3.1.1.14 beta-carotene in absence of other lipids, inhibits chlorophyl a conversion 858 3.1.1.14 4-hydroxymercuribenzoate 93% inhibition at 1 mM, 24 h preincubation 970 3.1.1.14 4-hydroxymercuribenzoate - 970 3.1.1.14 Melatonin melatonin treatment of seeds enhances the ability of pea seedlings to accelerate chlorophyll breakdown and chlorophyll de novo synthesis before stress by herbicide paraquat treatment appears and several hours after stressing, respectively, while during prolonged paraquat incubation, melatonin delays chlorophyll degradation 1536 3.1.1.14 concanavalin A - 2045 3.1.1.14 phytol in methanol, hydrolysis of chlorophyll a 2351 3.1.1.14 phytol uncompetitive inhibition 2351 3.1.1.14 phytol - 2351 3.1.1.14 levulinic acid inhibits the chlorophyll synthesis in greening leaves, alters the chlorophyll a/chlorophyll b ratio 8266 3.1.1.14 pheophytin b substrate inhibition above 0.005 mM 12996 3.1.1.14 Bi3+ 52% residual activity at 10 mM 15661 3.1.1.14 Bi3+ 58% residual activity at 10 mM 15661 3.1.1.14 Bi3+ 55% residual activity at 10 mM 15661 3.1.1.14 phosphatidyl glycerol and sulfoquinovosyldiacylglycerol, inactivation in a cooperative manner 20850 3.1.1.14 canola oil presence of 10%, 20%, and 30% of canola oil decreased the activity of entrapped chlorophyllase by 60%, 75%, and 80%, respectively 66045 3.1.1.14 sulfoquinovosyldiacylglycerol and phosphatidyl glycerol, inactivation in a cooperative manner 97196 3.1.1.14 refined bleached deodorized canola oil 20% 129627 3.1.1.14 Eupergit C immobilization support 129628 3.1.1.14 Eupergit C/EDA immobilization support 129629