2.8.1.6	5'-deoxyadenosine	strong inhibitor, reversible, 90% inhibition upon addition of 1 equivalent of 5'-deoxyadenosine with regard to the enzyme	943	
2.8.1.6	5'-deoxyadenosine	product inhibition. The combination of both products in equimolar concentrations results in more than simple additive inhibition, suggesting that they bind cooperatively to the enzyme.	943	
2.8.1.6	5'-deoxyadenosine	competitive, inhibits the growth of Mycobacterium tuberculosis H37Ra with an MIC of 392.6 microg/ml	943	
2.8.1.6	acidomycin	structural analog of biotin, complete inhibition at 0.4 mM, 50% inhibition at 0.035 mM	115159	
2.8.1.6	acidomycin	acidomycin inhibits biotin synthesis through the competitive inhibition of biotin synthase BioB and also stimulates unproductive cleavage of S-adenosyl-L-methionine to generate the toxic metabolite 5'-deoxyadenosine. Acidomycin selectively accumulates in Mycobycterium tuberculosis. The development of spontaneous resistance by Mycobacterium tuberculosis to acidomycin is difficult, and only low-level resistance to acidomycin is observed by overexpression of BioB	115159	
2.8.1.6	Cd2+	1 mM, almost complete inhibition	52	
2.8.1.6	Co2+	1 mM, almost complete inhibition	23	
2.8.1.6	Cu2+	1 mM, almost complete inhibition	19	
2.8.1.6	Fe2+	above 1 mM	25	
2.8.1.6	Hg2+	1 mM, almost complete inhibition	33	
2.8.1.6	L-methionine	product inhibition. The combination of both products in equimolar concentrations results in more than simple additive inhibition, suggesting that they bind cooperatively to the enzyme.	88	
2.8.1.6	additional information	not inhibitory: pyridoxal 5’-phosphate, deoxyadenosine	2	
2.8.1.6	additional information	5'-methylthioadenosine is not an inhibitor. Neither biotin nor iminobiotin is a significant inhibitor	2	
2.8.1.6	NaBH4	decreases cysteine desulfurase and biotin synthase activity	616	
2.8.1.6	S-(5'-adenosyl)-L-cysteine	competitive	155286	
2.8.1.6	S-(5'-adenosyl)-L-homocysteine	competitive	107214	
2.8.1.6	S-adenosyl-L-homocysteine	potent inhibitor	36	
2.8.1.6	sinefungin	-	659	
2.8.1.6	Zn2+	1 mM, almost complete inhibition	14