2.7.1.19 3-phosphoglycerate 74% inhibition at 6 mM and pH 7.2 194 2.7.1.19 5,5'-dithiobis(2-nitrobenzoic acid) DTNB, Ellman’s reagent, almost complete inhibition at 0.0045 mM 221 2.7.1.19 6-phospho-D-gluconate - 830 2.7.1.19 6-phospho-D-gluconate competitive with respect to D-ribulose 5-phosphate 830 2.7.1.19 6-phosphogluconate competitive inhibitor, 78% residual activity at 1 mM 391 2.7.1.19 ADP competitive, 1 mM 13 2.7.1.19 ADP - 13 2.7.1.19 AMP competitive, 1 mM 30 2.7.1.19 AMP 50% inhibition at 0.08 mM 30 2.7.1.19 AMP - 30 2.7.1.19 Ca2+ 200 mM 15 2.7.1.19 chloroplast protein 12 - 4747 2.7.1.19 Co2+ 200 mM 23 2.7.1.19 Cu2+ - 19 2.7.1.19 cystine oxidized form, inhibition almost completely reversible by addition of reduced dithiothreitol 1967 2.7.1.19 D-Ribulose 1,5-diphosphate - 6044 2.7.1.19 daphnetin i.e. 7,8-dihydroxycoumarin 8088 2.7.1.19 diethyl dicarbonate 27% residual activity at 0.04 mM 463 2.7.1.19 dithiobis-(2-nitrobenzoic acid) complete inactivation, 3 mM ATP protects 16464 2.7.1.19 dithiothreitol oxidized form 45 2.7.1.19 dithiothreitol light- and dark-adapted extract prepared and assayed without reducing agent, is not significantly affected by incubation with reduced dithiothreitol, however, subsequent incubation with oxidized dithiothreitol results in a significant reduction of enzyme activity under both light and dark conditions 45 2.7.1.19 DL-glyceraldehyde non competitive, 10 mM 487 2.7.1.19 glutathione oxidized form, inhibition almost completely reversible by addition of reduced dithiothreitol 44 2.7.1.19 glutathione oxidized form 44 2.7.1.19 H2O2 complete inactivation, 3 mM ATP protects 22 2.7.1.19 Hg2+ - 33 2.7.1.19 iodoacetate - 93 2.7.1.19 L-aspartate competitive, 1 mM 97 2.7.1.19 L-malate competitive, 1 mM 247 2.7.1.19 Mn2+ 200 mM 11 2.7.1.19 additional information inhibited by addition of strong oxidants, reversible by addition of reducing agents 2 2.7.1.19 additional information oxidizing agents inhibit enzyme activity 2 2.7.1.19 additional information not inhibited by 7-methyl-8-hydroxycoumarin 2 2.7.1.19 p-chloromercuribenzoate - 43 2.7.1.19 phosphoenolpyruvate 50% inhibition at 0.32 mM 51 2.7.1.19 phosphoenolpyruvate competitive, 1 mM 51 2.7.1.19 phosphoenolpyruvate - 51 2.7.1.19 pyridoxal 5'-phosphate - 32 2.7.1.19 pyridoxal 5'-phosphate inactivation is protected by ATP in combination with NADH 32 2.7.1.19 regulatory protein CP12 forming under oxidizing conditions a supramolecular complex (2 phosphoribulokinase dimers + 2 glyceraldehyde-3-phosphate dehydrogenase tetramers + 4 CP12 monomers) 155464