2.4.1.41	acetonitrile	10% (v/v) acetonitrile reduces enzyme activity to about 60%	981	
2.4.1.41	ADP	weak	13	
2.4.1.41	alphaS1-casein	strong inhibition of GalNAc transfer to kappa-casein, reversed by Ca2+	31174	
2.4.1.41	AMP	-	30	
2.4.1.41	ATP	weak	4	
2.4.1.41	benzyl N-acetyl-alpha-D-galactosamine	-	210446	
2.4.1.41	benzyl-alpha-GalNAc	-	14363	
2.4.1.41	benzyl-N-acetyl-alpha-D-galactosaminide	benzyl-GalNAc, 2 mM over night in Hek293T cell culture, abolishes activity of transiently transfected xGalntl-1 in Hek293T cells like inhibition of Smad2 phosphorylation by ActR-IIB and induction of a migration shift of ActR-IIB and Alk-3	143824	
2.4.1.41	CDP	-	212	
2.4.1.41	CMP	-	100	
2.4.1.41	CTP	-	60	
2.4.1.41	Cu2+	-	19	
2.4.1.41	D-galactose	-	71	
2.4.1.41	diethyldicarbonate	binds to His; hydroxylamine partiallly protects, UDP-GalNAc protects	310	
2.4.1.41	EDTA	10 mM, complete inhibition	21	
2.4.1.41	EDTA	0.5 mM, complete inhibition	21	
2.4.1.41	EDTA	Na2EDTA, 7.5 mM, complete inhibition	21	
2.4.1.41	EDTA	GalNAc-T4 enzyme and lectin domain; inhibition of GalNAc-T2 enzyme and lectin domain	21	
2.4.1.41	EDTA	no activity in the presence of EDTA	21	
2.4.1.41	fully GalNAc-substituted MUC1 repeat peptide	inhibits activity of rGalNAc-T4 and -T2	110946	
2.4.1.41	GalNAc	inhibition of GalNAc-T1 by free GalNAc: follow-up O-glycosylation of apomucin is sensitive, O-glycosylation of synthetic peptides containing a single glycosylation site is resistant	4928	
2.4.1.41	GalNAc	inhibition for GalNAc-T2 lectin domain to bind GalNAc-MUC1; inhibition for GalNAc-T4 lectin domain to bind GalNAc-MUC1	4928	
2.4.1.41	GDP	weak	53	
2.4.1.41	GMP	-	162	
2.4.1.41	GTP	-	37	
2.4.1.41	Helix pomatia lectin	-	99187	
2.4.1.41	HIV-V3	competitive inhibition of Muc2 glycosylation	111034	
2.4.1.41	methyl-alpha-Gal	-	69477	
2.4.1.41	methyl-beta-Gal	-	69478	
2.4.1.41	Mn2+	Mn2+ is the best activator, maximal activity at 5 mM, 20 and 100 mM result in 10% and 80% inhibition	11	
2.4.1.41	Mn2+	at higher concentrations, 30 mM: 50% inhibition	11	
2.4.1.41	Mn2+	inhibitory at concentrations higher than 80 mM	11	
2.4.1.41	additional information	not inhibited by ATP, CDP, GDP	2	
2.4.1.41	additional information	not inhibited by wheat germ lectin	2	
2.4.1.41	additional information	not inhibited by UTP	2	
2.4.1.41	additional information	elongation of GalNAc by beta3-galactosylation inhibits rGalNAc-T4 activity completely and rGalNAc-T2 activity with respect to the acceptor site GSTA, not inhibited by GlcNAc	2	
2.4.1.41	additional information	GalNAc-T1: follow-up glycosylation is inhibited by addition of beta1,3Gal to GalNAc, apomucin glycosylation is not inhibited by galactose, glucose, N-acetylglucosamine, mannose and fucose	2	
2.4.1.41	additional information	-	2	
2.4.1.41	additional information	benzyl or phenyl alpha and beta-GalNAc compounds show higher inhibition for GalNAc-T2 to bind GalNAc-MUC1 than for isoform GalNAc-T4. No inhibition of GalNAc-T2 enzyme and lectin domain by glucose, GlcNAc or mannose; benzyl or phenyl alpha and beta-GalNAc compounds show weaker inhibition for GalNAc-T4 to bind GalNAc-MUC1 than for isoform GalNAc-T2. No inhibition of GalNAc-T4 enzyme and lectin domain by glucose, GlcNAc or mannose	2	
2.4.1.41	additional information	not inhibited by by D-glucose or UDP-N-acetyl-alpha-D-galactosamine	2	
2.4.1.41	additional information	addition of 5% (v/v) of acetonitrile or the addition of up to 10% (v/v) methanol or glycerol has no negative effect on enzyme activity	2	
2.4.1.41	Muc2	competitive inhibition of HIV-V3 peptide glycosylation	13348	
2.4.1.41	N-acetyl-alpha-D-galactosamine	the acetylated enzyme is strongly inhibited by N-acetyl-alpha-D-galactosamine	2057	
2.4.1.41	o-nitrophenyl-alpha-GalNAc	-	14365	
2.4.1.41	o-nitrophenyl-beta-GalNAc	-	18670	
2.4.1.41	p-chloromercuriphenylsulfonic acid	irreversible	1416	
2.4.1.41	p-nitrophenyl-alpha-GalNAc	-	18671	
2.4.1.41	p-nitrophenyl-beta-GalNAc	-	14366	
2.4.1.41	phenyl-alpha-GalNAc	-	14364	
2.4.1.41	Soybean lectin	-	98746	
2.4.1.41	UDP	competitive	26	
2.4.1.41	UDP	strong	26	
2.4.1.41	UDP-alpha-GalNAc	-	14390	
2.4.1.41	UMP	uncompetitive	133	
2.4.1.41	UMP	-	133	
2.4.1.41	UMP-1-hexadecanesulfonic anhydride	50% inhibition at 0.16 mM, competitive	111878	
2.4.1.41	UMP-1-octanesulfonic anhydride	-	97282	
2.4.1.41	UMP-butanesulfonic anhydride	-	97284	
2.4.1.41	UTP	competitive	65	
2.4.1.41	UTP	-	65