2.4.1.22 2,4,6-Trinitrobenzenesulfonate modification of alpha-lactalbumin 3303 2.4.1.22 2-deoxy-D-glucose 0.4 M, 68% inhibition 531 2.4.1.22 2-Hydroxy-5-nitrobenzyl bromide modification of alpha-lactalbumin 3778 2.4.1.22 2-mercaptoethanol modification of alpha-lactalbumin 63 2.4.1.22 2-Nitrophenylsulfenyl chloride modification of alpha-lactalbumin 91137 2.4.1.22 acetone 20% v/v, 69% inhibition 333 2.4.1.22 acetonitrile 20% v/v, complete inhibition 981 2.4.1.22 alpha-lactalbumin inhibits galactosyl transfer to N-acetylglucosamine 1247 2.4.1.22 Ca2+ above 4 mM 15 2.4.1.22 carbodiimide at pH 4.5 rapid loss of the biological activity of alpha-lactalbumin 5948 2.4.1.22 D-arabinose 0.4 M, 13% inhibition 565 2.4.1.22 D-ribose 0.4 M, 59% inhibition 292 2.4.1.22 D-xylose 0.4 M, 36% inhibition 115 2.4.1.22 Dimethyl-(2-hydroxy-5-nitrobenzyl)-sulfonium bromide modification of alpha-lactalbumin 93210 2.4.1.22 Dimethylsulfoxide 20% v/v, 43% inhibition 985 2.4.1.22 dioxane 20% v/v, 88% inhibition 2960 2.4.1.22 dithiothreitol the rate of reduction of the disulfide bond in alpha-lactalbumin is much faster with dithiothreitol than mercaptoethanol, and at 24°C proceeds to completion within minutes at pH 8 45 2.4.1.22 ethanol 20% v/v, 4% inhibition 69 2.4.1.22 Formylkynurenine - 9163 2.4.1.22 glycinamide at pH 4.5 rapid loss of the biological activity of alpha-lactalbumin 8113 2.4.1.22 Iodine - 1619 2.4.1.22 iodoacetate modification of alpha-lactalbumin, carboxymethylation proceeds in the order His68, His32, His107. The activity decreases progressively as carboxymethylation proceeds, the fully carboxymethylated derivative has about 40% of the activity of the unmodified alpha-lactalbumin in lactose synthetase assay 93 2.4.1.22 iodoacetate - 93 2.4.1.22 L-arabinose 0.4 M, 54% inhibition 206 2.4.1.22 L-sorbose 0.4 M, 45% inhibition 685 2.4.1.22 L-Xylose 0.4 M, 16% inhibition 1927 2.4.1.22 Maleic anhydride - 8132 2.4.1.22 Mg2+ above 4 mM 6 2.4.1.22 additional information alpha-lactalbumin strongly inhibits the transfer of galactose to free N-acetylglucosamine 2 2.4.1.22 N,N-Dimethylformamide 20% v/v, 90% inhibition 5998 2.4.1.22 N-Acetylimidazole - 1559 2.4.1.22 N-methylpyrrolidone 20% v/v, complete inhibition 62461 2.4.1.22 tetrahydrofuran 20% v/v, complete inhibition 2760 2.4.1.22 Tetranitromethane - 1453 2.4.1.22 tunicamycin - 1659 2.4.1.22 UDPglucose - 186 2.4.1.22 vincristine - 6613