1.7.1.6 3,3'-methylene-bis(4-hydroxycoumarin) dicoumarol 3265 1.7.1.6 4-Dimethylaminoazobenzene - 91683 1.7.1.6 Ag+ 1 mM, 92% inhibition 75 1.7.1.6 Al3+ 2 mM, 47.9% residual activity 264 1.7.1.6 Cd2+ IC50: 0.13 mM 52 1.7.1.6 Cd2+ complete inhibition at 25 mM 52 1.7.1.6 cetyl dimethylethyl ammonium bromide - 220842 1.7.1.6 Cetylpyridinium chloride - 9125 1.7.1.6 Cibacron blue a competitive NAD(P)H inhibitor 1138 1.7.1.6 CO noncompetitive vs. amaranth 176 1.7.1.6 Cu2+ 1 mM, 88% inhibition 19 1.7.1.6 Cu2+ - 19 1.7.1.6 Cu2+ 2 mM, 84.6% residual activity 19 1.7.1.6 dicoumarol 0.0001 mM, 50% inhibition of methyl red reduction, 0.0004 mM, 90% inhibition, competitive vs. NADPH noncompetitve vs. methyl red 754 1.7.1.6 dicoumarol - 754 1.7.1.6 fatty acids oxidized unsaturated 2739 1.7.1.6 Fe2+ 1 mM, 90% inhibition 25 1.7.1.6 Fe2+ IC50: 0.11 mM 25 1.7.1.6 Fe2+ complete inhibition 25 1.7.1.6 Fe3+ 1 mM, 85% inhibition 70 1.7.1.6 ferric chloride 1 mM leads to 80% inhibition, 2.5 mM leads to 98% inhibition 135356 1.7.1.6 Hg2+ 1 mM, 75% inhibition 33 1.7.1.6 Hg2+ IC50: 0.17 mM 33 1.7.1.6 Hg2+ - 33 1.7.1.6 iodoacetamide loss of 30.6% activity at 10% v/v 67 1.7.1.6 Methyl red substrate inhibition above 0.08 mM 989 1.7.1.6 Mg2+ 2 mM, 81% residual activity 6 1.7.1.6 Mn2+ IC50: 0.43 mM 11 1.7.1.6 Mn2+ - 11 1.7.1.6 Mn2+ inhibits at 25 mM 11 1.7.1.6 additional information not inhibited by N-ethylmaleimide, p-chloromercuribenzoate and monoiodoacetic acid 2 1.7.1.6 additional information 0.08% SDS together with 0.0022 mM KCN causes 50% inhibition 2 1.7.1.6 additional information changes in salinity from 0 to 2% do not affect the activity of the enzyme very much. After a 1-h incubation, more than 80% relative activity is indicated at 0-5% salt concentrations 2 1.7.1.6 additional information the enzyme is oxygen-insensitive 2 1.7.1.6 NaCl more than 40% of maximum activity up to 2.5 M 42 1.7.1.6 NaCl the wild type enzyme is completely inactivated by 5% (w/v) NaCl after 24 h incubation 42 1.7.1.6 Orange I substrate inhibition 10740 1.7.1.6 Orange II substrate inhibition at high concentration 9236 1.7.1.6 oxygen presence of oxygen in the assay reduces the activity 1180 1.7.1.6 SDS 1 mM, 50% inhibition 124 1.7.1.6 SDS - 124 1.7.1.6 sodium dodecylsulfate 1 mM, 53% residual activity 1066 1.7.1.6 tetradecylammonium bromide - 220843 1.7.1.6 Zn2+ IC50: 0.28 mM 14 1.7.1.6 Zn2+ 5 mM, 30% residual activity 14 1.7.1.6 Zn2+ inhibits at 25 mM 14