1.4.1.13 2',5'-ADP act as a competitive ihibitor 1962 1.4.1.13 2',5'-diphosphoadenylic acid competitive inhibitor with respect to NADPH 48455 1.4.1.13 2'-adenylic acid competitive inhibitor of the forward reaction with NADPH as the varied substrate, uncompetitive inhibitor with alpha-ketoglutarate or L-glutamine 7976 1.4.1.13 2'-adenylic acid - 7976 1.4.1.13 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, uncompetitive inhibition with ferricyanide or iodonitrotetrazolium as substrate, competitive inhibition with NADPH as substrate 11091 1.4.1.13 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose inhibitor of the NADPH:iodonitrotetrazolium oxidoreductase reaction of the G298A-beta subunit, competitive with respect to NADPH 11091 1.4.1.13 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose - 11091 1.4.1.13 2'-phosphoadenylic acid competitive inhibitor with respect to NADPH 48458 1.4.1.13 2,2'-bipyridyl 100% inhibition at 3 mM 1706 1.4.1.13 2,2'-bipyridyl 88% inhibition at 10 mM 1706 1.4.1.13 3-Aminopyridine adenine dinucleotide phosphate good inhibitor of the holoenzyme, competitive with respect to NADPH 5906 1.4.1.13 3-Aminopyridine adenine dinucleotide phosphate inhibitor of the NADPH:iodonitrotetrazolium oxidoreductase reaction of the G298A-beta subunit, competitive with respect to NADPH 5906 1.4.1.13 3-Aminopyridine adenine dinucleotide phosphate - 5906 1.4.1.13 3-Bromo-2-oxoglutarate - 19969 1.4.1.13 4-Iodoacetamidosalicylate inactivation is faster at pH 4.6 to 5.5 compared to pH 6.5 to 7.2 43450 1.4.1.13 4-Iodoacetamidosalicylate - 43450 1.4.1.13 6-diazo-5-oxo-L-norleucine potent inhibitor, complete loss of activity at 0.1 mM after 10 min of preincubation 839 1.4.1.13 6-diazo-5-oxo-L-norleucine 35% inhibition at 0.0025 mM, when reaction is initiated with 0.5 mM L-glutamine, 73% inhibition at 0.0025 mM, when reaction is initiated with 2.5 mM 2-oxoglutarate 839 1.4.1.13 acetyl-NADP+ - 32137 1.4.1.13 ADP 15% inhibition at 50 mM 13 1.4.1.13 ADP - 13 1.4.1.13 AgNO3 at 0.001 mM completely inhibits 360 1.4.1.13 alpha-aminobutyrate - 92370 1.4.1.13 AMP - 30 1.4.1.13 arginine less than 45% inhibition 532 1.4.1.13 Atebrin 100% inhibition at 1 mM 2721 1.4.1.13 ATP 19% inhibition at 25 mM and 43% inhibition at 50 mM 4 1.4.1.13 ATP at 3.5 mM 12% inhibition 4 1.4.1.13 ATP 33% inhibition at 20 mM 4 1.4.1.13 ATP restricts activity by 47% at 5 mM 4 1.4.1.13 azaserine in both the L-glutamine- and NH4Cl-dependent reactions 1861 1.4.1.13 azaserine 68% inhibition at 1 mM 1861 1.4.1.13 azaserine 38% inhibition at 0.0125 mM when reaction is initiated with 0.5 mM L-glutamine and 69% inhibition at 0.0125 mM when reaction is initiated with 2.5 mM 2-oxoglutarate 1861 1.4.1.13 BaCl2 61% inhibition at 50 mM 1275 1.4.1.13 Bromopyruvate - 1917 1.4.1.13 Butane-2,3-dione slight inactivation 8058 1.4.1.13 CaCl2 74% inhibition at 50 mM 218 1.4.1.13 CaCl2 - 218 1.4.1.13 CaCl2 27% inhibition at 50 mM 218 1.4.1.13 CdCl2 produces more than 60% inhibition at 5 mM 906 1.4.1.13 Cibacron blue 3GA - 5140 1.4.1.13 cis-aconitate 22% inhibition at 50 mM 1230 1.4.1.13 citrate slight 131 1.4.1.13 CoCl2 produces more than 60% inhibition at 5 mM 414 1.4.1.13 CTP 9% inhibition at 25 mM and 21% inhibition at 50 mM 60 1.4.1.13 D-glutamate 50% inhibition at concentrations below 7 mM 344 1.4.1.13 D-glutamate - 344 1.4.1.13 D-glutamate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with L-glutamine and uncompetitive with NADPH 344 1.4.1.13 D-glutamate at 3.5 mM 10% inhibition 344 1.4.1.13 D-glutamate no inhibition: 50-200 mM 344 1.4.1.13 D-glutamine - 2138 1.4.1.13 D-Lysine significantly inhibits 1531 1.4.1.13 D-methionine 19% inhibition at 50 mM 957 1.4.1.13 dimethyl suberimidate inactivates glutamine-dependent activity 48291 1.4.1.13 DL-methionine DL-sulfoximine potent inhibitor 13170 1.4.1.13 DL-methionine DL-sulfoximine - 13170 1.4.1.13 DL-methionine sulfoxide potent inhibitor 93282 1.4.1.13 DL-Theanine - 93287 1.4.1.13 fumarate 21% inhibition at 50 mM 170 1.4.1.13 fumarate potent inhibitor of the glutamine-dependent activity 170 1.4.1.13 glycine significant inhibitor 72 1.4.1.13 glycine less than 50% inhibition at 5 mM 72 1.4.1.13 glycine 5% inhibition at 5 mM 72 1.4.1.13 GTP 16% inhibition at 25 mM and 43% inhibition at 50 mM 37 1.4.1.13 HgCl2 at 0.001 mM remains 8% of activity 110 1.4.1.13 hydroxylamine - 85 1.4.1.13 iodoacetamide more than 50% loss in activity in 60 min at 0.5 mM 67 1.4.1.13 iodoacetamide - 67 1.4.1.13 iodoacetamide selective inactivation of glutamine-dependent activity 67 1.4.1.13 iodoacetate completely inhibits at 1 mM, 2-mercaptoethanol protects against inhibition 93 1.4.1.13 iodoacetate 27% inhibition at 25 mM 93 1.4.1.13 iodonitrotetrazolium inhibitor of the L-glutamate:iodonitrotetrazolim oxidoreductase activity of the alpha subunit at concentrations above 0.1 mM 6738 1.4.1.13 isocitrate 35% inhibition at 50 mM 279 1.4.1.13 isocitrate 35% inhibition at 5 mM 279 1.4.1.13 isocitrate 32% inhibition at 20 mM 279 1.4.1.13 ITP 17% inhibition at 25 mM and 29% inhibition at 50 mM 229 1.4.1.13 KBr 43% inhibition at 100 mM 2746 1.4.1.13 KBr - 2746 1.4.1.13 KCl 32% inhibition at 100 mM 79 1.4.1.13 KCN 89% inhibition at 20 mM 161 1.4.1.13 KI - 1979 1.4.1.13 KNO3 47% inhibition at 100 mM 2273 1.4.1.13 L-2-Amino-4-oxo-5-chloropentanoic acid selective inhibitor of glutamine-dependent activity 5985 1.4.1.13 L-alanine significant inhibitor 103 1.4.1.13 L-alanine less than 50% inhibition at 5 mM 103 1.4.1.13 L-alanine - 103 1.4.1.13 L-alanine 5% inhibition at 5 mM 103 1.4.1.13 L-asparagine significant inhibitor 266 1.4.1.13 L-asparagine less than 50% inhibition at 5 mM 266 1.4.1.13 L-asparagine activity decreases to about 0.5% of the original at 25 mM 266 1.4.1.13 L-aspartate 50% inhibition at concentrations below 7 mM 97 1.4.1.13 L-aspartate - 97 1.4.1.13 L-cysteine significant inhibitor 74 1.4.1.13 L-DON - 135325 1.4.1.13 L-glutamate 50% inhibition at concentrations between 17 and 35 mM 41 1.4.1.13 L-glutamate potent inhibitor 41 1.4.1.13 L-glutamate competitive inhibitor of the forward reaction with L-glutamine as the varied substrate, noncompetitive inhibitor with alpha-ketoglutarate as the varied substrate 41 1.4.1.13 L-glutamate at 3.5 mM 13% inhibition 41 1.4.1.13 L-glutamate more than 40% inhibition at 50 mM 41 1.4.1.13 L-glutamate 30% inhibition at 500 mM 41 1.4.1.13 L-glutamate noncompetitive inhibitor with respect to both 2-oxoglutarate and L-glutamine 41 1.4.1.13 L-histidine significant inhibitor 349 1.4.1.13 L-histidine less than 50% inhibition at 5 mM 349 1.4.1.13 L-histidine - 349 1.4.1.13 L-histidine 5% inhibition at 5 mM 349 1.4.1.13 L-histidine more than 40% inhibition at 50 mM 349 1.4.1.13 L-homoserine significant inhibitor 345 1.4.1.13 L-homoserine - 345 1.4.1.13 L-leucine less than 50% inhibition at 5 mM 127 1.4.1.13 L-leucine - 127 1.4.1.13 L-leucine 16% inhibition at 5 mM 127 1.4.1.13 L-leucine less than 45% inhibition 127 1.4.1.13 L-malate 19% inhibition at 50 mM 247 1.4.1.13 L-malate more than 50% inhibition at 5 mM 247 1.4.1.13 L-malate at 3.5 mM 67% inhibition 247 1.4.1.13 L-malate potent inhibitor of the glutamine-dependent activity 247 1.4.1.13 L-malate 40% inhibition at 5 mM 247 1.4.1.13 L-methionine 50% inhibition at concentrations below 7 mM 88 1.4.1.13 L-methionine shows no effect 88 1.4.1.13 L-methionine - 88 1.4.1.13 L-methionine competitive inhibitor with respect to L-glutamine 88 1.4.1.13 L-methionine 16% inhibition at 5 mM 88 1.4.1.13 L-methionine more than 40% inhibition at 50 mM 88 1.4.1.13 L-methionine DL-sulfoximine potent inhibitor 48304 1.4.1.13 L-methionine DL-sulfoximine inhibits the glutamine-dependent reaction 48304 1.4.1.13 L-methionine sulfone competitive inhibitor vs. L-glutamine, noncompetitive inhibitor vs. alpha-ketoglutarate and uncompetitive vs. NADPH 3081 1.4.1.13 L-methionine sulfone potent inhibitor 3081 1.4.1.13 L-methionine sulfone - 3081 1.4.1.13 L-methionine sulfone reversible inhibitor 3081 1.4.1.13 L-serine significant inhibitor 95 1.4.1.13 L-serine less than 50% inhibition at 5 mM 95 1.4.1.13 L-serine 10% inhibition at 5 mM 95 1.4.1.13 L-serine more than 40% inhibition at 10 mM and 50 mM 95 1.4.1.13 L-tryptophan strong inhibitor 119 1.4.1.13 lysine significantly inhibits 1107 1.4.1.13 lysine - 1107 1.4.1.13 methionine sulfone - 9215 1.4.1.13 MgCl2 70% inhibition at 50 mM 196 1.4.1.13 MgCl2 31% inhibition at 50 mM 196 1.4.1.13 MnCl2 82% inhibition at 50 mM 307 1.4.1.13 MnCl2 64% inhibition at 50 mM 307 1.4.1.13 additional information relatively insensitive to inhibition by amino acids, keto acids or various nucleotides, 38% inhibition with a combination of each 5 mM L-methionine, L-leucine, L-serine, L-histidine, L-glycine and L-alanine 2 1.4.1.13 additional information a combination of L-serine, L-methionine, L-alanine, L-glycine, L-histidine and L-asparagine inhibits 73% of the acitivity at 7.5 mM each 2 1.4.1.13 N-ethylmaleimide 83% inhibition at 10 mM 49 1.4.1.13 N-ethylmaleimide - 49 1.4.1.13 NaClO4 - 9230 1.4.1.13 NAD+ competitive inhibition with NADH, noncompetitive inhibition with 2-oxoglutarate or L-glutamine 7 1.4.1.13 NADH at 3.5 mM 31% inhibition 8 1.4.1.13 NADH partially inhibits 8 1.4.1.13 NADP+ 50% inhibition at concentrations below 7 mM 10 1.4.1.13 NADP+ potent inhibitor 10 1.4.1.13 NADP+ competitive vs. NADPH and noncompetitive vs. L-glutamine 10 1.4.1.13 NADP+ 76% inhibition 10 1.4.1.13 NADP+ competitive inhibition with respect to NADPH; potent inhibitor 10 1.4.1.13 NADP+ 59% inhibition at 1 mM 10 1.4.1.13 NADP+ competitive inhibition with NADPH, noncompetitive inhibition with 2-oxoglutarate or L-glutamine 10 1.4.1.13 NADP+ competitive inhibitor with NADPH, 54% inhibition at 1 mM 10 1.4.1.13 NADP+ inhibitor of the NADPH:iodonitrotetrazolium oxidoreductase reaction of the G298A-beta subunit, competitive with respect to NADPH 10 1.4.1.13 NADP+ - 10 1.4.1.13 NADPH - 5 1.4.1.13 NADPH rapid inactivation in absence of L-glutamine and 2-oxoglutarate, capable of causing 50% inactivation in 5 min at a concentration of 1 mM 5 1.4.1.13 NADPH inactivation of glutamine-dependent activity, 50% inactivation at 0.36 mM in 10 min 5 1.4.1.13 NaN3 100% inhibition at 50 mM, 11% inhibition at 20 mM 238 1.4.1.13 NiCl2 produces more than 60% inhibition at 5 mM 741 1.4.1.13 O-carbamoylserine competitive inhibitor vs. L-glutamine 106906 1.4.1.13 o-phenanthroline 37% inhibition at 10 mM 239 1.4.1.13 o-phenanthroline complete inhibition at 10 mM 239 1.4.1.13 oxalate 48% inhibition at 5 mM 185 1.4.1.13 oxalglycine - 8416 1.4.1.13 oxaloacetate - 57 1.4.1.13 oxaloacetate more than 50% inhibition at 20 mM 57 1.4.1.13 oxalylglycine competitive inhibitor vs. alpha-ketoglutarate, noncompetitive inhibitor vs. L-glutamine and uncompetitive vs. NADPH 2304 1.4.1.13 p-chloromercuribenzoate completely inhibits at 1 mM, 2-mercaptoethanol protects against inhibition 43 1.4.1.13 p-chloromercuribenzoate - 43 1.4.1.13 p-chloromercuribenzoate inhibits the glutamine-dependent reaction 43 1.4.1.13 p-chloromercuribenzoate complete inhibition at 0.1 mM 43 1.4.1.13 p-chloromercuriphenylsulfonate more than 50% loss in activity in 60 min at 0.05 mM 1650 1.4.1.13 p-hydroxymercuribenzoate complete inhibition at 1 mM, 5 min 98 1.4.1.13 phenylalanine significantly inhibits 429 1.4.1.13 phenylalanine 78% inhibition at 10 mM 429 1.4.1.13 Phenylglyoxal at 3 mM pyridoxal 5'-phosphate glutamine-dependent glutamate synthase is inactivated by about 50% within 10 min. This inactivation is not prevented by 2-oxoglutarate 301 1.4.1.13 putrescine - 155 1.4.1.13 pyridoxal-5'-phosphate 52% inhibition of the glutamine-dependent reaction at 5 mM 4558 1.4.1.13 pyruvate 20% inhibition at 50 mM 31 1.4.1.13 pyruvate - 31 1.4.1.13 sodium arsenite 100% inhibition at 50 mM , 10% inhibition at 20 mM 2419 1.4.1.13 sodium arsenite 57% inhibition at 10 mM 2419 1.4.1.13 succinate 16% inhibition at 50 mM 58 1.4.1.13 succinate 25% inhibition at 5 mM 58 1.4.1.13 succinate - 58 1.4.1.13 thio-NADP+ - 2037 1.4.1.13 UTP 17% inhibition at 25 mM and 33% inhibition at 50 mM 65 1.4.1.13 valine - 1210 1.4.1.13 valine less than 45% inhibition 1210