1.17.1.9 1,10-phenanthroline - 62 1.17.1.9 1-chloro-2-oxopropane 20 mM, pH 6.5, 30°C, 5 h, 76% loss of activity 122936 1.17.1.9 1-methyl-3-methylimidazolium dimethylphosphate adding more than 40% (v/v) of 1-methyl-3-methylimidazolium dimethylphosphate inactivates formate dehydrogenase. In the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, the catalytic efficiency of the wild type enzyme is reduced by 2.8fold 174582 1.17.1.9 2,3-Butanedione modification of 12 Arg residues per molecule results in complete inactivation. NAD+ protects 1001 1.17.1.9 2-chloro-1-(3-pyridyl)-ethanone 20 mM, pH 6.5, 30°C, 5 h, 10% loss of activity 122937 1.17.1.9 4-hydroxybutyl acetate IC50 of 15.6 vol% 174583 1.17.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) - 2373 1.17.1.9 5-Nitro-8-hydroxyquinoline - 91950 1.17.1.9 ADP inhibition is greater at acidic pH than at neutral pH 13 1.17.1.9 ADP 86% inhibition at 50 mM; 90% inhibition at 50 mM 13 1.17.1.9 ADP-beta-D-ribose competitive with NAD+, mixed-type with formate 36318 1.17.1.9 ADPribose - 1069 1.17.1.9 AgNO3 0.01 mM 360 1.17.1.9 AgNO3 1 mM; 80% inhibition 360 1.17.1.9 AMP - 30 1.17.1.9 ATP inhibition is greater at acidic pH than at neutral pH 4 1.17.1.9 ATP 45% inhibition at 50 mM; 56% inhibition at 50 mM 4 1.17.1.9 azide - 230 1.17.1.9 azide 0.9 mM, 50% inhibition 230 1.17.1.9 azide uncompetitive with NAD+, competitive with formate 230 1.17.1.9 azide potent inhibitor 230 1.17.1.9 azide a transition-state analogue inhibitor of FDH 230 1.17.1.9 azide nanomolar inhibitor 230 1.17.1.9 benzaldehyde slight inhibition 146 1.17.1.9 bicarbonate mixed-type inhibition of NAD+ and formate 1136 1.17.1.9 Br- - 332 1.17.1.9 Ca2+ 61.51% residual activity at 10 mM 15 1.17.1.9 Cd2+ CdCl2 52 1.17.1.9 Cd2+ 1 mM CdSO4, 39% inhibition 52 1.17.1.9 Cd2+ complete inhibition at 1 mM 52 1.17.1.9 Cl- - 141 1.17.1.9 CN- - 159 1.17.1.9 CN- 0.25 mM 159 1.17.1.9 CN- 1 mM KCN, complete inhibition 159 1.17.1.9 CN- 0.1 mM NaCN, 90% inhibition 159 1.17.1.9 CN- NaCN 159 1.17.1.9 CN- 1 mM KCN, 50% inhibition 159 1.17.1.9 CO2 - 28 1.17.1.9 CO32- 16.93% residual activity at 100 mM 10616 1.17.1.9 Cu2+ - 19 1.17.1.9 Cu2+ 1 mM CuCl2, 81% inhibition 19 1.17.1.9 Cu2+ 0.2 mM, 13.6% inhibition 19 1.17.1.9 Cu2+ 1 mM, 37% inhibition 19 1.17.1.9 Cu2+ 82 inhibition at 1 mM; complete inhibition at 1 mM 19 1.17.1.9 Cu2+ 1 mM Cu2+ inhibits the enzyme activity by 79% 19 1.17.1.9 Cu2+ 94.2% inhibition at 1 mM 19 1.17.1.9 Cu2+ the wild type enzyme is completely inactivated by 5 mM Cu2+ 19 1.17.1.9 cyanide - 118 1.17.1.9 cycloserine weak 2837 1.17.1.9 dimethylimidazolium dimethyl phosphate FDh is inactivated and unstable in the presence of high concentration (above 50%) of the water soluble dimethylimidazolium dimethyl phosphate ionic liquid 163033 1.17.1.9 Dithionitrobenzoate - 6045 1.17.1.9 EDTA 50 mM, slight inhibition 21 1.17.1.9 ethanol IC50 of 4.8 vol% 69 1.17.1.9 ethyl 4-chloroacetoacetate the D221G mutant is extremely sensitive to ethyl 4-chloroacetoacetate with half-lives shorter than 6 min 7455 1.17.1.9 ethyl-4-chloroacetoacetate wild-type, 7.43% residual activity at 20 mM 135290 1.17.1.9 F- - 174 1.17.1.9 Fe3+ 83.5% inhibition at 1 mM 70 1.17.1.9 Ferron - 44130 1.17.1.9 H2PO4- 78.54% residual activity at 100 mM 30061 1.17.1.9 HCO3- - 195 1.17.1.9 Hg2+ - 33 1.17.1.9 Hg2+ 1 mM, complete inhibition 33 1.17.1.9 Hg2+ strong inhibition 33 1.17.1.9 Hg2+ 98% inhibition at 1 mM; 98% inhibition at 1 mM 33 1.17.1.9 hydrazine - 684 1.17.1.9 hydroxylamine - 85 1.17.1.9 imidazole - 470 1.17.1.9 iodoacetamide - 67 1.17.1.9 iodoacetamide weak 67 1.17.1.9 iodoacetate strong inhibition 93 1.17.1.9 iodoacetic acid weak 213 1.17.1.9 Isonicotinyl hydrazide weak 93730 1.17.1.9 Isopropanol IC50 of 4.7 vol% 958 1.17.1.9 KF 1 mM, 80% inhibition 2410 1.17.1.9 methanol - 83 1.17.1.9 methanol IC50 of 10.2 vol% 83 1.17.1.9 Mg2+ 89.93% residual activity at 10 mM 6 1.17.1.9 additional information no loss of activity after 5 h at pH 6.5, 30°C, after 5 h with 20 mM ethyl 4-chloro-3-oxobutanoate, 20 mM ethyl-4-bromo-3-oxobutanoate, 20 mM ethyl 2-chloro-3-oxobutanoate, or 20 mM 2,3'-dichloroacetophenone 2 1.17.1.9 additional information not inhibitory: Mg2+, Mn2+, Co2+, Ni2+, Cd2+, Zn2+, Ca2+, Fe2+, Fe3+, Cu2+ 2 1.17.1.9 additional information no notable effect on the enzyme activity is observed when adding NH4+ or HPO2- 2 1.17.1.9 n-butanol IC50 of 1.4 vol% 629 1.17.1.9 n-Propanol IC50 of 2.5 vol% 947 1.17.1.9 N3- - 582 1.17.1.9 N3- 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ 582 1.17.1.9 N3- 1 mM NaN3, 90% inhibition 582 1.17.1.9 N3- 0.1 mM 582 1.17.1.9 Na2S - 2087 1.17.1.9 Na2SO3 - 3653 1.17.1.9 NAD+ - 7 1.17.1.9 NADH competitive versus NAD+ 8 1.17.1.9 NADH competitive versus NAD+; noncompetitive versus formate 8 1.17.1.9 NADH - 8 1.17.1.9 NADH inhibition is partly overcome by addition of formate or NAD+ 8 1.17.1.9 NADH competitive with NADH, mixed-type with formate 8 1.17.1.9 NADH 66% inhibition at 0.12 mM; 73% inhibition at 0.12 mM 8 1.17.1.9 NADP+ strong inhibition 10 1.17.1.9 NADPH - 5 1.17.1.9 NADPH competitive 5 1.17.1.9 NADPH strong inhibition 5 1.17.1.9 NADPH 26% inhibition at 0.24 mM; 58% inhibition at 0.24 mM 5 1.17.1.9 NaHSO3 - 2751 1.17.1.9 NaN3 1 mM, 80% inhibition 238 1.17.1.9 NaN3 1 mM, complete inhibition 238 1.17.1.9 NEM - 89 1.17.1.9 NEM 1 mM, 25.3% inhibition 89 1.17.1.9 Ni2+ NiCl2 38 1.17.1.9 Ni2+ 1 mM Ni2+ inhibits the enzyme activity by 28% 38 1.17.1.9 Ni2+ 12.4% inhibition at 1 mM 38 1.17.1.9 nitrate competitive inhibitor 308 1.17.1.9 NO2- - 852 1.17.1.9 NO2- 1 mM, 5% inhibition 852 1.17.1.9 NO3- - 673 1.17.1.9 NO3- 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ 673 1.17.1.9 NO3- 1 mM, 13% inhibition 673 1.17.1.9 NO3- 10 mM KNO3, 38% inhibition 673 1.17.1.9 NO3- 40.89% residual activity at 100 mM 673 1.17.1.9 o-nitrobenzaldehyde slight inhibition 4106 1.17.1.9 OCN- - 8161 1.17.1.9 oxidized ADP inactivates by specific reaction at the nucleotide binding site, with negative cooperativity between subunits accounting for appearance of two phases of inactivation, protection by NAD+, NADH and ADP 103781 1.17.1.9 p-chloromercuribenzoate strong inhibition 43 1.17.1.9 p-chloromercuriphenylsulfonic acid - 1416 1.17.1.9 p-hydroxymercuribenzoate 0.25 mM 98 1.17.1.9 p-hydroxymercuribenzoate - 98 1.17.1.9 Pb2+ - 139 1.17.1.9 Pb2+ 24.33% residual activity at 10 mM 139 1.17.1.9 PCMB - 78 1.17.1.9 PCMB 0.2 mM, 10.7% inhibition 78 1.17.1.9 PCMB 0.2 mM, complete inhibition 78 1.17.1.9 phenol IC50 of 0.32 vol% 249 1.17.1.9 phenylmercuric acetate - 2497 1.17.1.9 PO43- 30.9% residual activity at 100 mM 867 1.17.1.9 pyridoxal 5'-phosphate slight inhibition 32 1.17.1.9 SCN- - 1032 1.17.1.9 Semicarbazide - 382 1.17.1.9 Sodium azide 94% inhibition at 1 mM; 99% inhibition at 1 mM 447 1.17.1.9 sodium hypophosphite 1 mM, 80% inhibition 107260 1.17.1.9 Thiosemicarbazide - 3495 1.17.1.9 Thiourea - 844 1.17.1.9 Zincon - 95380 1.17.1.9 Zn2+ 1 mM 63% inhibition 14 1.17.1.9 Zn2+ - 14 1.17.1.9 Zn2+ 55.5% inhibition at 1 mM 14