1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - 857 1.11.1.19 2-mercaptoethanol 55% residual activity at 1 mM; 70% residual activity at 1 mM 63 1.11.1.19 4-arbutin 24.2% inhibition at 1.0 mM, 66.4% inhibition at 50 mM 262238 1.11.1.19 acetonitrile less than 5% residual activity at 40% (v/v) 981 1.11.1.19 Ag+ 50% residual activity at 5 mM; 82% residual activity at 5 mM 75 1.11.1.19 ascorbate 39.5% inhibition at 0.05 mM, complete inhibition at 0.5 mM 94 1.11.1.19 azide 26.6% inhibition at 5 mM, 68.4% inhibition at 50 mM 230 1.11.1.19 Ba2+ 90% residual activity at 5 mM 111 1.11.1.19 Ba2+ about 85% inhibition at 1 mM 111 1.11.1.19 Ca2+ 84.7% residual activity at 5 mM 15 1.11.1.19 Cd2+ complete inhibition at 5 mM 52 1.11.1.19 Cd2+ complete inhibition at 1 mM 52 1.11.1.19 Co2+ 66% residual activity at 5 mM 23 1.11.1.19 Co2+ about 56% inhibition at 1 mM 23 1.11.1.19 diethyl dicarbonate less than 50% inhibition at 50 mM 463 1.11.1.19 Direct blue 71 - 125511 1.11.1.19 dithiothreitol 65% residual activity at 1 mM; 81% residual activity at 1 mM 45 1.11.1.19 DMSO less than 10% residual activity at 40% (v/v) 896 1.11.1.19 ethanol about 30% residual activity at 40% (v/v) 69 1.11.1.19 Fe2+ AnaPX is highly sensitive to Fe2+ (98.8% inhibition at 5 mM) 25 1.11.1.19 Fe2+ 85% residual activity at 5 mM 25 1.11.1.19 Fe3+ 10 mM, 33% residual activity 70 1.11.1.19 Fe3+ 35.9% residual activity at 5 mM 70 1.11.1.19 H2O2 DyP activity for Reactive Blue 5 is inhibited when the H2O2 concentration exceeds 0.2 mM at a fixed DyP concentration of 0.6 nM. At 2.8 nM of DyP, its activity for 1,4-diamino-2-sodium anthraquinone sulfonate decreases sharply when the H2O2 concentration exceeds 0.1 mM 22 1.11.1.19 H2O2 DyP is inactivated by excess H2O2 22 1.11.1.19 H2O2 maximum relative activity at final H2O2 concentrations between 0.4 and 0.8 mM, activity then gradually drops off. A residual activity of 30% still remains at 12.5 mM H2O2, and the enzyme is completely inactivated in the presence of 50 mM H2O2 22 1.11.1.19 H2O2 - 22 1.11.1.19 H2O2 42% inhibition at 0.01 mM, complete inhibition at 0.05 mM 22 1.11.1.19 H2O2 the protein becomes progressively inactivated with increasing incubation time and peroxide concentration, detecting the highest inactivation above 100000 molar equivalents. Around 80% inactivation is observed with 3000 molar equivalents in 5 min, and the protein is completely deactivated after 10 min. The activity of the recombinant protein falls below 20% in 60 min of incubation at all the hydrogen peroxide concentrations tested 22 1.11.1.19 H2O2 an increase of the hydrogen peroxide concentration of more than 0.1 mM leads to a continuous activity decrease 22 1.11.1.19 Hg2+ complete inhibition at 5 mM 33 1.11.1.19 Hg2+ complete inhibition at 1 mM 33 1.11.1.19 hydroxylamine 10% residual activity at 2 mM; 25% residual activity at 2 mM 85 1.11.1.19 KCN AnaPX shows very low sensitivity to KCN which causes only 9% and 14% inhibition of the activity at 1 and 10 mM, respectively 161 1.11.1.19 L-cysteine 33.9% inhibition at 0.02 mM, complete inhibition at 0.1 mM 74 1.11.1.19 L-cystine 10% residual activity at 2 mM; 25% residual activity at 2 mM 1218 1.11.1.19 Li+ 87% residual activity at 5 mM 152 1.11.1.19 Metabisulfite 93.5% inhibition at 0.5 mM, complete inhibition at 1.0 mM 5182 1.11.1.19 methanol about 40% residual activity at 40% (v/v) 83 1.11.1.19 Mn2+ 57.1% inhibition at 5 mM 11 1.11.1.19 Mn2+ 92.3% residual activity at 5 mM 11 1.11.1.19 additional information the enzyme is not sensitive to the prototypical catalase inhibitor 3-amino-1,2,4,-triazole in the presence of ascorbic acid (1.0 mM), metal chelating and sulfhydryl reagents do not significantly affect activity 2 1.11.1.19 additional information not inhibited by EDTA, SDS, and NaCl 2 1.11.1.19 NaN3 AnaPX is highly sensitive to NaN3 (82% inhibition at 10 mM) 238 1.11.1.19 phenylhydrazine AnaPX is highly sensitive to the suicide substrate phenylhydrazine (complete inhibition at 1 mM) 398 1.11.1.19 potassium cyanide 3% residual activity at 10 mM; complete inhibition at 10 mM 2095 1.11.1.19 potassium cyanide 13% inhibition at 1 mM 2095 1.11.1.19 Propanol less than 40% residual activity at 30% (v/v) 976 1.11.1.19 Reactive Blue 19 - 15122 1.11.1.19 SDS 27% inhibition at 20 mM; 68% residual activity at 20 mM; 73% residual activity at 20 mM 124 1.11.1.19 SDS no activity at 5 mM SDS 124 1.11.1.19 Sodium azide 5% residual activity at 10 mM; complete inhibition at 10 mM 447 1.11.1.19 Sodium azide 9% inhibition at 1 mM 447 1.11.1.19 Zn2+ 88% residual activity at 5 mM 14