1.1.1.71 (R)-2-hydroxypropiophenone - 166290 1.1.1.71 2-mercaptoethanol slight inhibition 63 1.1.1.71 4-chloromercuribenzoate 0.01 mM, complete inhibition 388 1.1.1.71 4-chloromercuribenzoate 0.01 mM, complete inhibition; some protection of partial inhibition resulting from 0.001 mM 4-chloromercuribenzoate in the presence of 1 mM NADP+, almost complete protection with 10 mM hexan-1-ol 388 1.1.1.71 4-chloromercuribenzoate 1 mM, 100% inhibition 388 1.1.1.71 4-chloromercuribenzoate - 388 1.1.1.71 8-hydroxyquinoline 1 mM, 100% inhibition with NAD+ as cofactor, 40% with NADP+ as cofactor 321 1.1.1.71 acetonitrile complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl 981 1.1.1.71 Ag+ - 75 1.1.1.71 AgNO3 1 mM, 100% inhibition 360 1.1.1.71 Ca2+ - 15 1.1.1.71 CdCl2 1 mM, 24% inhibition with NAD+ as cofactor 906 1.1.1.71 chloroform 21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl 4509 1.1.1.71 Co2+ - 23 1.1.1.71 Cu2+ - 19 1.1.1.71 CuSO4 1 mM, 75% inhibition with NAD+ as cofactor, 56% with NADP+ as cofactor 263 1.1.1.71 Cyclohexanol - 873 1.1.1.71 Dimethyl formamide 87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl 12511 1.1.1.71 dimethyl sulfoxide complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl 1217 1.1.1.71 EDTA 10 mM, 20% inhibition 21 1.1.1.71 EDTA 5 mM, 20% inhibition; 5 mM, 80% of initial activity 21 1.1.1.71 EDTA complete inhibition at 1 mM 21 1.1.1.71 ethyl acetate complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl 2738 1.1.1.71 Fe2+ 10 mM, 74% of initial activity 25 1.1.1.71 Fe2+ no activity at 1 mM 25 1.1.1.71 FeSO4 1 mM, 75% inhibition with NAD+ as cofactor, 46% with NADP+ as cofactor 655 1.1.1.71 FeSO4 10 mM, 26% inhibition 655 1.1.1.71 HgCl2 1 mM, 100% inhibition 110 1.1.1.71 iodoacetamide 1 mM, complete inhibition 67 1.1.1.71 iodoacetic acid 1 mM, 29% inhibition with NAD+ as cofactor, 80% with NADP+ as cofactor 213 1.1.1.71 methanol 75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl 83 1.1.1.71 Mg2+ 10 mM, 78% of initial activity 6 1.1.1.71 Mg2+ slight inhibition 6 1.1.1.71 additional information dithiothreitol and EDTA have no effect on either oxidative or reductive activity 2 1.1.1.71 additional information the Fe2+-reconstituted Ni-PhADH was sensitively and rapidly inactivated by dioxygen gas. The enzyme is inactivated by the replacement of the ferrous ion in the active site with another metal ion such as a zinc ion, which has been considered an inhibitor of iron-activating group III ADHs 2 1.1.1.71 n-decane 9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl 138675 1.1.1.71 N-ethylmaleimide 1 mM, complete inhibition 49 1.1.1.71 N-ethylmaleimide strong inhibition 49 1.1.1.71 n-hexane 40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl 14171 1.1.1.71 Ni2+ 14% residual activity at 1 mM 38 1.1.1.71 o-phenanthroline 1 mM, 100% inhibition with NAD+ as cofactor, 40% with NADP+ as cofactor 239 1.1.1.71 o-phenanthroline 1 mM, 30% inhibition after 3 h incubation 239 1.1.1.71 p-hydroxymercuribenzoate strong inhibition 98 1.1.1.71 Phenobarbital 0.5 mM, 30% inhibition 1475 1.1.1.71 pyrazole 0.5 mM, 35% inhibition 1078 1.1.1.71 Sodium azide 10 mM, 40% inhibition 447 1.1.1.71 Sodium glycocholate - 30408 1.1.1.71 Tween 80 - 514 1.1.1.71 Zn2+ 10 mM, 79% of initial activity 14 1.1.1.71 Zn2+ 14% residual activity at 1 mM 14 1.1.1.71 ZnSO4 10 mM, 21% inhibition 485